Rapid screening method to study the reactivity of UV filter substances towards skin proteins by high-performance thin-layer chromatography

2013 ◽  
Vol 35 (6) ◽  
pp. 588-599 ◽  
Author(s):  
C. Stiefel ◽  
W. Schwack
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Screening Method ◽  
Rapid Screening ◽  
Uv Filter ◽  
Rapid Screening Method ◽  
Layer Chromatography

Thin-Layer Chromatography as a Rapid Screening Method for the Determination of Carbaryl and 1-Naphthol Residues Without Cleanup

1964 ◽  
Vol 47 (4) ◽  
pp. 667-670
Author(s):  
M Chiba ◽  
H V Morley
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Screening Method ◽  
Rapid Screening ◽  
Detection And Estimation ◽  
Rapid Screening Method ◽  
Layer Chromatography

Abstract A rapid, sensitive, screening method has been developed in which thin-layer chromatography is used for the detection and estimation of carbaryl and l-naphthol residues in apple, lettuce, and tomato extracts without prior cleanup. A procedure giving a convenient screening range of 0.1—30 ppm is described, but 0.02 ppm in apples and lettuce and 0.03 ppm in tomatoes may be detected without cleanup. By use of a partitioning cleanup 5 ppb of carbaryl may be detected.

Rapid Screening Method for Deoxynivalenol in Agricultural Commodities by Fluorescent Minicolumn

1990 ◽  
Vol 73 (2) ◽  
pp. 266-270
Author(s):  
William C Gordon ◽  
Linda J Gordon
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Selective Adsorption ◽  
Screening Method ◽  
Rapid Screening ◽  
Blue Fluorescence ◽  
False Negatives ◽  
Rapid Screening Method ◽  
Gas Chromatography Mass Spectroscopy ◽  
Layer Chromatography

Abstract A rapid screening procedure based on the selective adsorption of deoxynivalenol (DON) from extracts of wheat and corn has been developed. DON is extracted from the sample with acetonitrile-water (85 + 15) and partially purified on a preparative minicolumn. Solvent is evaporated and the residue is dissolved in toluene-acetone (95 + 5) and chromatographed on a novel detector minicolumn which selectively adsorbs DON. A blue fluorescence is produced when the column is heated 5 min at 100°C. The procedure is capable of detecting DON at ≥ 500 ng/g. Forty-three wheat samples, contaminated with DON at 60-6300 ng/g, were assayed by gas chromatography-mass spectroscopy (GC-MS) of the heptafluorobutyryl derivative of DON and by the selective adsorption procedure. Comparison of results showed 9 1% agreement between data from the 2 methods. Selective adsorption assays were positive for all samples that were ≥ 500 ng/g by GC-MS (no false negatives) and were negative for 85 % of samples < 500 ng/g (4/27 false positives). These four samples contained > 200 ng/g by GC-MS. Samples of wheat (64), corn (23), soybeans (8), and sorghum (6) were extracted and extracts were assayed by thin-layer chromatography and the selective adsorption procedure. Selective adsorption assays agreed with TLC results.

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