High-performance thin-layer chromatographic quantification of three active compounds from the decoction of Abnormal Savda Munziq prescription

2014 ◽  
Vol 27 (3) ◽  
pp. 186-191
Author(s):  
Xuejia Zhang ◽  
Jie Li ◽  
Halmuart Upur ◽  
Shuge Tian
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Active Compounds ◽  
Abnormal Savda ◽  
Abnormal Savda Munziq

scholarly journals High-Performance Thin-Layer Chromatographic Quantification of Rosmarinic Acid and Rutin in Abnormal Savda Munziq

2013 ◽  
Vol 2013 ◽  
pp. 1-4
Author(s):  
S. G. Tian ◽  
L. D. Xin ◽  
Halmuart Upur
Keyword(s):  
Thin Layer ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Rapid Screening ◽  
Thin Layer Plate ◽  
Calibration Data ◽  
Linear Calibration ◽  
Color Reagent ◽  
Abnormal Savda ◽  
Abnormal Savda Munziq

A high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of rosmarinic acid and rutin in Abnormal Savda Munziq (ASMq). A methanol extract of ASMq was used for quantification. The compounds were separated on silica gel H thin layer plate with ethyl acetate-formic acid-acetic acid-water 15 : 1 : 1 : 1.5 (v/v) as a developer, trichloroethanol as the color reagent. The plates were scanned at 365 nm. The linear calibration data of rosmarinic acid and rutin were in the range of 0.0508 to 0.2540 μg(r=0.9964), 0.2707 to 1.35354μg(r=0.9981), respectively. The recovery rate of rosmarinic acid was 99.17% (RSD = 2.92%) and rutin was 95.24% (RSD = 2.38%). The method enables rapid screening, precise, selective, and sensitive quantification for pharmaceutical analysis.

Sign in / Sign up

Export Citation Format

Share Document