scholarly journals Optimization of Dairy Sludge for Growth ofRhizobiumCells

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Ashok Kumar Singh ◽  
Gauri Singh ◽  
Digvijay Gautam ◽  
Manjinder Kaur Bedi
Keyword(s):  
Optical Density ◽  
Yeast Extract ◽  
Rhizobium Meliloti ◽  
Environmental Parameters ◽  
Maximum Growth ◽  
Rhizobium Trifolii ◽  
Bacterial Strains ◽  
Yeast Extract Mannitol ◽  
Dairy Sludge ◽  
Synthetic Media

In this study dairy sludge was evaluated as an alternative cultivation medium forRhizobium. Growth of bacterial strains at different concentrations of Dairy sludge was monitored. Maximum growth of all strains was observed at 60% Dairy sludge concentration. At 60% optical density (OD) values are 0.804 forRhizobium trifolii(MTCC905), 0.825 forRhizobium trifolii(MTCC906), and 0.793 forRhizobium meliloti(MTCC100). Growth pattern of strains was observed at 60% Dairy sludge along with different synthetic media (tryptone yeast,Rhizobiumminimal medium and yeast extract mannitol). Growth in 60% Dairy sludge was found to be superior to standard media used forRhizobium. Media were optimized using 60% dairy sludge along with different concentrations of yeast extract (1–7 g/L) and mannitol (7–13 g/L) in terms of optical density at different time intervals, that is, 24, 48 and 72 hours. Maximum growth was observed in 6 g/L of yeast extract and 12 g/L of mannitol at 48-hour incubation period in all strains. The important environmental parameters such as pH were optimized using 60% dairy sludge, 60% dairy sludge +6 g/L yeast extract, and 60% dairy sludge +12 g/L mannitol. The maximum growth of all strains was found at pH 7.0. The present study recommends the use of 60% dairy sludge as a suitable growth medum for inoculant production.

THE INFLUENCE OF BIOS ON NODULE BACTERIA AND LEGUMES: B. INFLUENCE OF CRUDE BIOS PREPARATIONS ON ACID PRODUCTION BY STRAINS OF RHIZOBIUM TRIFOLII

1938 ◽  
Vol 16c (9) ◽  
pp. 347-353 ◽  
Author(s):  
D. G. Laird ◽  
P. M. West
Keyword(s):  
Vitamin C ◽  
Nicotinic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Vitamin B ◽  
Rhizobium Trifolii ◽  
Direct Proportion ◽  
Nodule Bacteria ◽  
Synthetic Media ◽  
Hydrolysis Of

Certain components of Wildiers' Bios complex, fractionated and concentrated according to the procedure of Miller and co-workers, were found capable of replacing the stimulative action of yeast extract on strains of Rhizobium trifolii, as measured by acid production. Bios I was inactive, while Bios II B, V, and II A possessed definite activity, the potency of the fractions increasing in the order named. Moreover, the ability of these fractions to increase hydrolysis of urea by urease was in direct proportion to the stimulative effect exerted by them on the Rhizobia. These effects could not be brought about in synthetic media by the addition of crystalline vitamin B1, nicotinic acid, uracil, choline, β-alanine, carnosine, β-indole acetic and β-indole butyric acids, glutathione, cysteine and vitamin C.

Studies on growth and symbiotic nitrogen fixation of Rhizobium of Vigna radiata under stress conditions

1984 ◽  
Vol 102 (2) ◽  
pp. 399-404
Author(s):  
R. Rai ◽  
V. Prasad
Keyword(s):  
Nitrogen Fixation ◽  
Yeast Extract ◽  
Vigna Radiata ◽  
Symbiotic Nitrogen Fixation ◽  
Alkaline Media ◽  
Growth Behaviour ◽  
Soil Stress ◽  
Wide Ph Range ◽  
Yeast Extract Mannitol ◽  
Ph Range

SummaryOne fast-growing acid-producing Rhizobium strain 995 of Vigna radiata was screened for growth behaviour in acid, saline and alkaline media. It grew well in yeast-extract mannitol broth of wide pH range as well as varying concentrations of NaCl, Na2SO4 and MnCl2. Variation in nodulation and N2-fixation efficiency occurred on different varieties of green gram, while strain characteristics were not affected by soil stress.

scholarly journals The Effect of pH Stress on the Survival of Rhizobium Trifolii and Sinorhizobium Meliloti in Vitro

2013 ◽  
Vol 70 (2) ◽  
pp. 449-450
Author(s):  
Monica NISTE ◽  
Roxana VIDICAN ◽  
Ioan ROTAR ◽  
Rodica POP
Keyword(s):  
Sinorhizobium Meliloti ◽  
Agar Medium ◽  
Rhizobium Trifolii ◽  
Ph Values ◽  
Optimum Ph ◽  
Yeast Extract Mannitol ◽  
Rhizobial Species ◽  
The Impact ◽  
Different Soils

Nitrogen-fixing symbiotic bacteria known as rhizobia can exist in different soils and adapt to different environmental conditions. The aim of this study was to determine the impact of pH on the growth of Rhizobium trifolii and Sinorhizobium meliloti. Rhizobial species were isolated using yeast extract mannitol agar medium) in which the pH values were adjusted to 5.0, 6.0, 8.0 and 9.0 by adding HCl and NaOH. The optimum pH for rhizobia is neutral or slightly alkaline (pH 8) and they are more sensitive to acidity. Sinorhizobium meliloti developed better in an acid medium compared to Rhizobium trifolii.

scholarly journals Interference between Rhizobium meliloti and Rhizobium trifolii nodulation genes: genetic basis of R. meliloti dominance.

1988 ◽  
Vol 170 (12) ◽  
pp. 5718-5727 ◽  
Author(s):  
F Debellé ◽  
F Maillet ◽  
J Vasse ◽  
C Rosenberg ◽  
F de Billy ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Rhizobium Meliloti ◽  
Rhizobium Trifolii ◽  
Nodulation Genes

Characterisation of biodegradable organic matter in reclaimed water using a bacterial growth fingerprint assay

2016 ◽  
Vol 16 (5) ◽  
pp. 1255-1265 ◽  
Author(s):  
Parinda Thayanukul ◽  
Futoshi Kurisu ◽  
Ikuro Kasuga ◽  
Kizuku Kanaya ◽  
Hiroaki Furumai
Keyword(s):  
Organic Matter ◽  
Bacterial Growth ◽  
Reclaimed Water ◽  
Maximum Growth ◽  
Bacterial Strains ◽  
Operational Conditions ◽  
Assimilable Organic Carbon ◽  
The Difference ◽  
Microbial Regrowth ◽  
Biodegradable Organic Matter

Microbial regrowth, microbial growth after disinfection, is an important problem that deteriorates water quality during the storage and distribution of reclaimed water. Biodegradable organic matter (BOM) that remains after water reclamation processes directly promotes microbial regrowth. In this study we propose a novel assay called the ‘bacterial growth fingerprint (BGF)’ to characterise BOM based on the maximum growth of bacterial strains, which is the extension of the conventional assimilable organic carbon assay for drinking water. Nine bacterial strains were selected from nearly 200 isolates from various reclaimed water systems. These selected bacterial strains exhibited unique substrate utilisation patterns. The BGF assay clearly reflected the difference in the quantity and quality of BOM between six different reclamation plants and the changes in BOM during a full-scale reclamation process. The information on BOM revealed by the BGF assay is useful to optimise the treatment processes or operational conditions for biologically stable reclaimed water.

scholarly journals Technique for Studying of the Dynamics of Changes of a Smoky Environment Parameters during the Transition of Pyrolysis to Flame Combustion

2019 ◽  
Vol 10 (4) ◽  
pp. 382-390
Author(s):  
A. A. Antoshyn ◽  
V. I. Nikitin
Keyword(s):  
Carbon Monoxide ◽  
Research Methods ◽  
Optical Density ◽  
Environmental Parameters ◽  
Test Fire ◽  
Specific Optical Density ◽  
Flame Combustion ◽  
Environment Parameters ◽  
First Time

The existing methods for monitoring the performance of multi-criteria fire detectors do not provide for verification of their characteristics in the conditions of transition from smoldering to flame burning. The aim of the work is the development of the research methods of the environmental parameters during the transition from smoldering (pyrolysis) to flame combustion for simulation a test fire while checking the quality of multi-criteria fire detectors.A technique to conduct research of environmental parameters under conditions of heating wood samples of different sizes to a temperature of selfignition and burning crumpled and smooth paper has been developed.Changes in the concentration of carbon monoxide, specific optical density, and scattering ability during the transition from smoldering (pyrolysis) to flame burning of prepared wood and crumpled paper were studied for the first time.It is shown that the controlled environmental parameters during the transition from smoldering pyrolysis to flame burning change together. Conclusion: the speed of growth of the scattering ability of smoke decreases by 2.4 times, the speed of increase in the specific optical density and concentration of carbon monoxide increases by 2 and 5.3 times (respec-tively), during the transition from pyrolysis to flame burning of wood.

THE APPLICATION OF A NUTRITIONAL GROUPING METHOD TO SOIL FUNGI

1955 ◽  
Vol 33 (3) ◽  
pp. 281-288 ◽  
Author(s):  
R. G. Atkinson ◽  
J. B. Robinson
Keyword(s):  
Amino Acids ◽  
Growth Factors ◽  
Yeast Extract ◽  
Soil Fungi ◽  
Basal Medium ◽  
Maximum Growth ◽  
Potassium Nitrate ◽  
Soil Extract ◽  
Mineral Salts ◽  
Soil Extracts

In tests with seven different liquid media in which the common nitrogen source was potassium nitrate and the carbohydrate substrate was glucose, at a concentration of only 0.1%, most of the 1914 soil fungi isolated fell into one of three nutritional groups requiring, respectively, for maximum growth amino acids, amino acids plus growth factors, or yeast extract. Relatively few isolates required growth factors alone or a combination of yeast and soil extracts. Most of the isolates grew poorly in the basal medium containing only mineral salts, and glucose, with or without soil extract. Although fungi requiring yeast extract were much less frequently isolated from soil on, rather than remote from, tubers grown in a soybean green-manured plot, isolates requiring amino acids, or yeast plus soil extracts, were correspondingly increased on immature and mature tubers, respectively. In a second plot, however, not specially treated, no differences were observed in the nutritional spectra of fungi isolated from the two kinds of soil environment.

Deciphering the Multi-Dimensional Abilities of Indigenous Bacteria Enterobacter Cloacae Isolated from Arsenic Contaminated Industrial Sites

2021 ◽  
Author(s):  
Reeta Bhati ◽  
Smitha Mony Sreedha ◽  
Rajni Singh
Keyword(s):  
Defense Mechanisms ◽  
Toxic Metal ◽  
Enterobacter Cloacae ◽  
Maximum Growth ◽  
Contaminated Site ◽  
Bacterial Strains ◽  
High Concentration ◽  
Industrial Sites ◽  
Zinc Cadmium ◽  
Cadmium Selenium

Abstract Arsenic (As) is a quintessential toxic metalloid and it has been classified as Group 1 human carcinogen. The evolution of arsenic defense mechanisms due to the omnipresent nature of arsenic has resulted in its alteration to less toxic forms. The present study deals with the isolation of arsenic remediating microbial strains from soil samples and their integration into bioremediation strategy. From the metal contaminated site, 118 different bacterial strains were isolated from heavy metal contaminated site. Twenty-five strains were tolerant to arsenic and one bacterial strain Enterobacter cloacae (RSC3) demonstrated maximum growth at high concentration of arsenate (6000ppm). The cell growth kinetics of RSC3revealed the specific growth rate (µ) to be 0.55 h-1. The The bacteria hosts arsC gene in the genome involved in the reduction of arsenate to arsenite. AAS, SEM, TEM and EDX studies confirmed the arsenate transportation and efflux of arsenic by the bacteria. Furthermore, the strain showed multi-resistance to other heavy metals like zinc, cadmium, selenium and nickel and several antibiotics indicating its application for facilitating bioremediation of toxic metal contaminated sites.

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