THE INFLUENCE OF BIOS ON NODULE BACTERIA AND LEGUMES: B. INFLUENCE OF CRUDE BIOS PREPARATIONS ON ACID PRODUCTION BY STRAINS OF RHIZOBIUM TRIFOLII
Certain components of Wildiers' Bios complex, fractionated and concentrated according to the procedure of Miller and co-workers, were found capable of replacing the stimulative action of yeast extract on strains of Rhizobium trifolii, as measured by acid production. Bios I was inactive, while Bios II B, V, and II A possessed definite activity, the potency of the fractions increasing in the order named. Moreover, the ability of these fractions to increase hydrolysis of urea by urease was in direct proportion to the stimulative effect exerted by them on the Rhizobia. These effects could not be brought about in synthetic media by the addition of crystalline vitamin B1, nicotinic acid, uracil, choline, β-alanine, carnosine, β-indole acetic and β-indole butyric acids, glutathione, cysteine and vitamin C.