scholarly journals Anxiolytic-Like Actions of Fatty Acids Identified in Human Amniotic Fluid

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Rosa Isela García-Ríos ◽  
Juan Francisco Rodríguez-Landa ◽  
Carlos M. Contreras
Keyword(s):  
Fatty Acids ◽  
Locomotor Activity ◽  
Amniotic Fluid ◽  
Natural Environment ◽  
Wistar Rats ◽  
Motor Coordination ◽  
Human Amniotic Fluid ◽  
Intrauterine Development ◽  
Defensive Burying ◽  
Dose Response Effect

Eight fatty acids (C12–C18) were previously identified in human amniotic fluid, colostrum, and milk in similar proportions but different amounts. Amniotic fluid is well known to be the natural environment for development in mammals. Interestingly, amniotic fluid and an artificial mixture of fatty acids contained in amniotic fluid produce similar anxiolytic-like actions in Wistar rats. We explored whether the lowest amount of fatty acids contained in amniotic fluid with respect to colostrum and milk produces such anxiolytic-like effects. Although a trend toward a dose-response effect was observed, only an amount of fatty acids that was similar to amniotic fluid fully mimicked the effect of diazepam (2 mg/kg, i.p.) in the defensive burying test, an action devoid of effects on locomotor activity and motor coordination. Our results confirm that the amount of fatty acids contained in amniotic fluid is sufficient to produce anxiolytic-like effects, suggesting similar actions during intrauterine development.

scholarly journals Myristic Acid Produces Anxiolytic-Like Effects in Wistar Rats in the Elevated Plus Maze

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Carlos M. Contreras ◽  
Juan Francisco Rodríguez-Landa ◽  
Rosa Isela García-Ríos ◽  
Jonathan Cueto-Escobedo ◽  
Gabriel Guillen-Ruiz ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Locomotor Activity ◽  
Amniotic Fluid ◽  
Myristic Acid ◽  
Wistar Rats ◽  
Elevated Plus Maze ◽  
Acid Mixture ◽  
Human Amniotic Fluid ◽  
Plus Maze

A mixture of eight fatty acids (linoleic, palmitic, stearic, myristic, elaidic, lauric, oleic, and palmitoleic acids) at similar concentrations identified in human amniotic fluid produces anxiolytic-like effects comparable to diazepam in Wistar rats. However, individual effects of each fatty acid remain unexplored. In Wistar rats, we evaluated the separate action of each fatty acid at the corresponding concentrations previously found in human amniotic fluid on anxiety-like behaviour. Individual effects were compared with vehicle, an artificial mixture of the same eight fatty acids, and a reference anxiolytic drug (diazepam, 2 mg/kg). Myristic acid, the fatty acid mixture, and diazepam increased the time spent in the open arms of the elevated plus maze and reduced the anxiety index compared with vehicle, without altering general locomotor activity. The other fatty acids had no effect on anxiety-like behaviour, but oleic acid reduced locomotor activity. Additionally, myristic acid produced anxiolytic-like effects only when the concentration corresponded to the one identified in human amniotic fluid (30 𝜇g/mL) but did not alter locomotor activity. We conclude that of the eight fatty acids contained in the fatty acid mixture, only myristic acid produces anxiolytic-like effects when administered individually at a similar concentration detected in human amniotic fluid.

Anxiolytic-like effects of human amniotic fluid and its fatty acids in wistar rats

2011 ◽  
Vol 22 (7) ◽  
pp. 655-662 ◽  
Author(s):  
Carlos M. Contreras ◽  
Juan Francisco Rodríguez-Landa ◽  
Ana G. Gutiérrez-García ◽  
M. Remedios Mendoza-López ◽  
Rosa Isela García-Ríos ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Amniotic Fluid ◽  
Wistar Rats ◽  
Human Amniotic Fluid

scholarly journals A Fatty Acids Mixture Reduces Anxiety-Like Behaviors in Infant Rats Mediated by GABAA Receptors

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Blandina Bernal-Morales ◽  
Jonathan Cueto-Escobedo ◽  
Gabriel Guillén-Ruiz ◽  
Juan F. Rodríguez-Landa ◽  
Carlos M. Contreras
Keyword(s):  
Fatty Acids ◽  
Locomotor Activity ◽  
Wistar Rats ◽  
Elevated Plus Maze ◽  
Acid Mixture ◽  
Gabaergic Neurotransmission ◽  
Infant Rats ◽  
Plus Maze ◽  
Defensive Burying ◽  
Young Offspring

Fatty acids (C6–C18) found in human amniotic fluid, colostrum, and maternal milk reduce behavioral indicators of experimental anxiety in adult Wistar rats. Unknown, however, is whether the anxiolytic-like effects of fatty acids provide a natural mechanism against anxiety in young offspring. The present study assessed the anxiolytic-like effect of a mixture of lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, elaidic acid, and linoleic acid in Wistar rats on postnatal day 28. Infant rats were subjected to the elevated plus maze, defensive burying test, and locomotor activity test. Diazepam was used as a reference anxiolytic drug. A group that was pretreated with picrotoxin was used to explore the participation of γ-aminobutyric acid-A (GABAA) receptors in the anxiolytic-like effects. Similar to diazepam, the fatty acid mixture significantly increased the frequency of entries into and time spent on the open arms of the elevated plus maze and decreased burying behavior in the defensive burying test, without producing significant changes in spontaneous locomotor activity. These anxiolytic-like effects were blocked by picrotoxin. Results suggest that these fatty acids that are contained in maternal fluid may reduce anxiety-like behavior by modulating GABAergic neurotransmission in infant 28-day-old rats.

scholarly journals Amniotic Fluid or Its Fatty Acids Produce Actions Similar to Diazepam on Lateral Septal Neurons Firing Rate

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Ana G. Gutiérrez-García ◽  
Carlos M. Contreras ◽  
Diana Idania Vásquez-Hernández
Keyword(s):  
Fatty Acids ◽  
Social Behavior ◽  
Amniotic Fluid ◽  
Firing Rate ◽  
Social Recognition ◽  
Vehicle Group ◽  
Human Amniotic Fluid ◽  
Adult Rats ◽  
Anxiolytic Drugs ◽  
Septal Neurons

Human amniotic fluid (AF) contains eight fatty acids (FATs), and both produce anxiolytic-like effects in adult rats and appetitive responses in human newborns. The medial amygdala and lateral septal nucleus function are related to social behavior, but the action of AF or its FATs in this circuit is known. We obtained 267 single-unit extracellular recordings in Wistar rats treated with vehicle (1 mL, s.c.;n=12), human AF (1 mL, s.c.;n=12), a FAT mixture (1 mL, s.c.;n=13), diazepam (1 mg/kg, i.p.;n=11), and fluoxetine (1 mg/kg, p.o.;n=12). Compared with the vehicle group, the spontaneous septal firing rate in the AF, FAT mixture, and diazepam groups was the lowest and in the fluoxetine group the highest. Cumulative peristimulus histograms indicated that the significant change in septal firing occurred only in the AF and FAT mixture groups and exclusively in those neurons that increased their firing rate during amygdala stimulation. We conclude that human AF and its FATs produce actions comparable to anxiolytic drugs and are able to modify the responsivity of a circuit involved in social behavior, suggesting facilitation of social recognition processes by maternal-fetal fluids.

Physical exercise protects dynamic balance and motor coordination of rats treated with vincristine

2020 ◽  
Vol 19 (5) ◽  
pp. 336
Author(s):  
Luiza Minato Sagrillo ◽  
Viviane Nogueira De Zorzi ◽  
Luiz Fernando Freire Royes ◽  
Michele Rechia Fighera ◽  
Beatriz Da Silva Rosa Bonadiman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Locomotor Activity ◽  
Physical Exercise ◽  
Motor Coordination ◽  
Dynamic Balance ◽  
Exercise Group ◽  
Adult Rats ◽  
The Central Nervous System ◽  
Stress Damage ◽  
Training Protocol

Physical exercise has been shown to be an important modulator of the antioxidant system and neuroprotective in several diseases and treatments that affect the central nervous system. In this sense, the present study aimed to evaluate the effect of physical exercise in dynamic balance, motor coordination, exploratory locomotor activity and in the oxidative and immunological balance of rats treated with vincristine (VCR). For that, 40 adult rats were divided into two groups: exercise group (6 weeks of swimming, 1h/day, 5 days/week, with overload of 5% of body weight) and sedentary group. After training, rats were treated with 0.5 mg/kg of vincristine sulfate for two weeks or with the same dose of 0.9% NaCl. The behavioral tests were conducted 1 and 7 days after each dose of VCR. On day 15 we carried out the biochemical analyzes of the cerebellum. The physical exercise was able to protect against the loss of dynamic balance and motor coordination and, had effect per se in the exploratory locomotor activity, and neutralize oxidative stress, damage DNA and immune damage caused by VCR up to 15 days after the end of the training protocol. In conclusion, we observed that previous physical training protects of the damage motor induced by vincristine.Key-words: exercise, oxidative stress, neuroprotection, cerebellum.

The Consumption of Galactomannan Effervescent Drinks made from Coconut Pulp Waste and Colonic Microbiota in Wistar Rats

2020 ◽  
Vol 15 (1) ◽  
pp. 52-56
Author(s):  
Sri Winarti ◽  
Agung Pasetyo
Keyword(s):  
Fatty Acids ◽  
Gut Microbiota ◽  
Wistar Rats ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
E Coli ◽  
Colonic Microbiota ◽  
Male Wistar Rats ◽  
Lactobacillus Spp ◽  
Chain Fatty Acids

The consumption of prebiotics is known to affect the balance of gut microbiota. The purpose of this study was to explore how a galactomannan-rich effervescent drink can affect the population of Lactobacillus, Bifidobacterium, E. coli, and the concentration of short-chain fatty acids in the cecum of rats. Twenty-eight male Wistar rats (aged 2 months) were divided equally into 7 groups and treated orally each day for 15 days with 2 mL effervescent drinks with increasing levels of prebiotic galactomannan. The dosage of 500 mg galactomannan increased the growth of Lactobacillus spp. and Bifidobacterium spp. with inhibition of the growth of E.coli with increased formation of short-chain fatty acids such as acetate, propionate, and butyrate in the cecum of rats.

scholarly journals Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats

2021 ◽  
Vol 139 ◽  
pp. 111691
Author(s):  
Jelica D. Grujić-Milanović ◽  
Zoran Z. Miloradović ◽  
Nevena D. Mihailović-Stanojević ◽  
Vojislav V. Banjac ◽  
Strahinja Vidosavljević ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Wistar Rats ◽  
Unsaturated Fatty Acids

scholarly journals Condrogenesis of mesenchymal stem cells derived from human amniotic fluid in chitosan-xanthan scaffolds under TGF- beta 3 stimuli

2019 ◽  
Vol 27 ◽  
pp. S433-S434
Author(s):  
I.I. Damas ◽  
C.C. Zuliani ◽  
Â.M. Moraes ◽  
C.B. Westin ◽  
K.C. Andrade ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Tgf Beta ◽  
Human Amniotic Fluid
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