scholarly journals Three-Dimensional Reconstruction of Coronary Arteries and Its Application in Localization of Coronary Artery Segments Corresponding to Myocardial Segments Identified by Transthoracic Echocardiography

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Chunyan Zhong ◽  
Yanli Guo ◽  
Haiyun Huang ◽  
Liwen Tan ◽  
Yi Wu ◽  
...  
Keyword(s):  
Coronary Arteries ◽  
Transthoracic Echocardiography ◽  
3D Structure ◽  
Three Dimensional ◽  
3D Models ◽  
Localization Accuracy ◽  
Significant Difference ◽  
2D Ultrasound ◽  
Ct Data ◽  
Contrast Ct

Objectives.To establish 3D models of coronary arteries (CA) and study their application in localization of CA segments identified by Transthoracic Echocardiography (TTE).Methods.Sectional images of the heart collected from the first CVH dataset and contrast CT data were used to establish 3D models of the CA. Virtual dissection was performed on the 3D models to simulate the conventional sections of TTE. Then, we used 2D ultrasound, speckle tracking imaging (STI), and 2D ultrasound plus 3D CA models to diagnose 170 patients and compare the results to coronary angiography (CAG).Results.3D models of CA distinctly displayed both 3D structure and 2D sections of CA. This simulated TTE imaging in any plane and showed the CA segments that corresponded to 17 myocardial segments identified by TTE. The localization accuracy showed a significant difference between 2D ultrasound and 2D ultrasound plus 3D CA model in the severe stenosis group (P<0.05) and in the mild-to-moderate stenosis group (P<0.05).Conclusions.These innovative modeling techniques help clinicians identify the CA segments that correspond to myocardial segments typically shown in TTE sectional images, thereby increasing the accuracy of the TTE-based diagnosis of CHD.

Maternal-Fetal Bonding: Ultrasound Imaging's Role in enhancing This Important Relationship

2012 ◽  
Vol 6 (4) ◽  
pp. 408-411 ◽  
Author(s):  
Kallie Appleton ◽  
Aparna Atluru
Keyword(s):  
Obstet Gynecol ◽  
New Technology ◽  
Relevant Literature ◽  
Three Dimensional ◽  
Unborn Child ◽  
Fetal Demise ◽  
4D Ultrasound ◽  
Important Relationship ◽  
Significant Difference ◽  
2D Ultrasound

ABSTRACT New technology in ultrasound imaging is allowing women to view more visually precise images of their fetuses than ever before. Maternal-fetal bonding describes the attachment interaction that forms between a mother and her unborn child. Ultrasound diagnosis modalities including two-dimensional (2D), three-dimensional (3D) and four-dimensional (4D) may create differences in the amount of maternal-fetal bonding, depending on the modality used. When relevant literature was reviewed on this topic, no significant difference between maternal-fetal bonding was found when comparing 2D vs 3D vs 4D ultrasound. However, certain measures such as a perceived feeling of closeness to the baby were higher with 3D and 4D ultrasounds as compared with 2D ultrasound. Further exploration is needed to ascertain whether different ultrasound modalities have an effect on maternal-fetal bonding in multigestational pregnancies, pregnancies in which there is fetal demise, and to overall examine the effects of using ultrasound for nonmedical ‘entertainment’ purposes by prospective mothers. How to cite this article Atluru A, Appleton K, Kupesic Plavsic S. Maternal-Fetal Bonding: Ultrasound Imaging's Role in enhancing This Important Relationship. Donald School J Ultrasound Obstet Gynecol 2012;6(4):408-411.

scholarly journals Neosuture formation after endoscope-assisted craniosynostosis repair

2016 ◽  
Vol 18 (2) ◽  
pp. 196-200 ◽  
Author(s):  
Afshin Salehi ◽  
Katherine Ott ◽  
Gary B. Skolnick ◽  
Dennis C. Nguyen ◽  
Sybill D. Naidoo ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Head Width ◽  
Long Term Outcomes ◽  
Sagittal Synostosis ◽  
Helmet Therapy ◽  
Age At Surgery ◽  
Significant Difference ◽  
Ct Data ◽  
Strip Craniectomy

OBJECTIVE The goal of this study was to identify the rate of neosuture formation in patients with craniosynostosis treated with endoscope-assisted strip craniectomy and investigate whether neosuture formation in sagittal craniosynostosis has an effect on postoperative calvarial shape. METHODS The authors retrospectively reviewed 166 cases of nonsyndromic craniosynostosis that underwent endoscope-assisted repair between 2006 and 2014. Preoperative and 1-year postoperative head CT scans were evaluated, and the rate of neosuture formation was calculated. Three-dimensional reconstructions of the CT data were used to measure cephalic index (CI) (ratio of head width and length) of patients with sagittal synostosis. Regression analysis was used to calculate significant differences between patients with and without neosuture accounting for age at surgery and preoperative CI. RESULTS Review of 96 patients revealed that some degree of neosuture development occurred in 23 patients (23.9%): 16 sagittal, 2 bilateral coronal, 4 unilateral coronal, and 1 lambdoid synostosis. Complete neosuture formation was seen in 14 of those 23 patients (9 of 16 sagittal, 1 of 2 bilateral coronal, 3 of 4 unilateral coronal, and 1 of 1 lambdoid). Mean pre- and postoperative CI in the complete sagittal neosuture group was 67.4% and 75.5%, respectively, and in the non-neosuture group was 69.8% and 74.4%, respectively. There was no statistically significant difference in the CI between the neosuture and fused suture groups preoperatively or 17 months postoperatively in patients with sagittal synostosis. CONCLUSIONS Neosuture development can occur after endoscope-assisted strip craniectomy and molding helmet therapy for patients with craniosynostosis. Although the authors did not detect a significant difference in calvarial shape postoperatively in the group with sagittal synostosis, the relevance of neosuture formation remains to be determined. Further studies are required to discover long-term outcomes comparing patients with and without neosuture formation.

scholarly journals Reconstruction of Three-Dimensional Conformations of Bacterial ClpB from High-Speed Atomic-Force-Microscopy Images

2021 ◽  
Vol 8 ◽  
Author(s):  
Bhaskar Dasgupta ◽  
Osamu Miyashita ◽  
Takayuki Uchihashi ◽  
Florence Tama
Keyword(s):  
Atomic Force Microscopy ◽  
Computational Modeling ◽  
High Speed ◽  
3D Structure ◽  
Three Dimensional ◽  
3D Models ◽  
Computational Method ◽  
Molecular Surface ◽  
Force Microscopy ◽  
Atomic Force

ClpB belongs to the cellular disaggretase machinery involved in rescuing misfolded or aggregated proteins during heat or other cellular shocks. The function of this protein relies on the interconversion between different conformations in its native condition. A recent high-speed-atomic-force-microscopy (HS-AFM) experiment on ClpB from Thermus thermophilus shows four predominant conformational classes, namely, open, closed, spiral, and half-spiral. Analyses of AFM images provide only partial structural information regarding the molecular surface, and thus computational modeling of three-dimensional (3D) structures of these conformations should help interpret dynamical events related to ClpB functions. In this study, we reconstruct 3D models of ClpB from HS-AFM images in different conformational classes. We have applied our recently developed computational method based on a low-resolution representation of 3D structure using a Gaussian mixture model, combined with a Monte-Carlo sampling algorithm to optimize the agreement with target AFM images. After conformational sampling, we obtained models that reflect conformational variety embedded within the AFM images. From these reconstructed 3D models, we described, in terms of relative domain arrangement, the different types of ClpB oligomeric conformations observed by HS-AFM experiments. In particular, we highlighted the slippage of the monomeric components around the seam. This study demonstrates that such details of information, necessary for annotating the different conformational states involved in the ClpB function, can be obtained by combining HS-AFM images, even with limited resolution, and computational modeling.

scholarly journals A novel 3D template for mandible and maxilla reconstruction: Rapid prototyping using stereolithography

2015 ◽  
Vol 48 (03) ◽  
pp. 263-273 ◽  
Author(s):  
Samir Kumta ◽  
Monica Kumta ◽  
Leena Jain ◽  
Shrirang Purohit ◽  
Rani Ummul
Keyword(s):  
Rapid Prototyping ◽  
Computer Aided Design ◽  
Treatment Plan ◽  
3D Structure ◽  
Three Dimensional ◽  
Bone Grafts ◽  
3D Models ◽  
Mirror Image ◽  
Anatomical Model ◽  
Patient Counselling

ABSTRACT Introduction: Replication of the exact three-dimensional (3D) structure of the maxilla and mandible is now a priority whilst attempting reconstruction of these bones to attain a complete functional and aesthetic rehabilitation. We hereby present the process of rapid prototyping using stereolithography to produce templates for modelling bone grafts and implants for maxilla/mandible reconstructions, its applications in tumour/trauma, and outcomes for primary and secondary reconstruction. Materials and Methods: Stereolithographic template-assisted reconstruction was used on 11 patients for the reconstruction of the mandible/maxilla primarily following tumour excision and secondarily for the realignment of post-traumatic malunited fractures or deformity corrections. Data obtained from the computed tomography (CT) scans with 1-mm resolution were converted into a computer-aided design (CAD) using the CT Digital Imaging and Communications in Medicine (DICOM) data. Once a CAD model was constructed, it was converted into a stereolithographic format and then processed by the rapid prototyping technology to produce the physical anatomical model using a resin. This resin model replicates the native mandible, which can be thus used off table as a guide for modelling the bone grafts. Discussion: This conversion of two-dimensional (2D) data from CT scan into 3D models is a very precise guide to shaping the bone grafts. Further, this CAD can reconstruct the defective half of the mandible using the mirror image principle, and the normal anatomical model can be created to aid secondary reconstructions. Conclusion: This novel approach allows a precise translation of the treatment plan directly to the surgical field. It is also an important teaching tool for implant moulding and fixation, and helps in patient counselling.

scholarly journals A Novel Three-Dimensional Vector Analysis of Axial Globe Position in Thyroid Eye Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Jie Guo ◽  
Jiang Qian ◽  
Yifei Yuan ◽  
Rui Zhang ◽  
Wenhu Huang
Keyword(s):  
Three Dimensional ◽  
Thyroid Eye Disease ◽  
Eye Disease ◽  
Vector Method ◽  
Significant Difference ◽  
The Difference ◽  
Ct Data ◽  
Ct Measurement ◽  
Geometrical Equation ◽  
Corneal Apex

Purpose. To define a three-dimensional (3D) vector method to describe the axial globe position in thyroid eye disease (TED).Methods. CT data from 59 patients with TED were collected and 3D images were reconstructed. A reference coordinate system was established, and the coordinates of the corneal apex and the eyeball center were calculated to obtain the globe vectorEC→. The measurement reliability was evaluated. The parameters ofEC→were analyzed and compared with the results of two-dimensional (2D) CT measurement, Hertel exophthalmometry, and strabismus tests.Results. The reliability ofEC→measurement was excellent. The difference betweenEC→and 2D CT measurement was significant (p=0.003), andEC→was more consistent with Hertel exophthalmometry than with 2D CT measurement (p<0.001). There was no significant difference betweenEC→and Hirschberg test, and a strong correlation was found betweenEC→and synoptophore test. When one eye had a larger deviation angle than its fellow, its corneal apex shifted in the corresponding direction, but the shift of the eyeball center was not significant. The parameters ofEC→were almost perfectly consistent with the geometrical equation.Conclusions. The establishment of a 3D globe vector is feasible and reliable, and it could provide more information in the axial globe position.

Validation of a method to measure three-dimensional hip joint kinematics in subjects with femoroacetabular impingement

2019 ◽  
pp. 112070001988354
Author(s):  
Thomas R Ward ◽  
Mafruha M Hussain ◽  
Mark Pickering ◽  
Diana Perriman ◽  
Al Burns ◽  
...  
Keyword(s):  
Femoroacetabular Impingement ◽  
Hip Joint ◽  
Gold Standard ◽  
Dynamic Assessment ◽  
Three Dimensional ◽  
3D Models ◽  
Joint Kinematics ◽  
Registration Method ◽  
Kinematic Measurement ◽  
Significant Difference

Introduction: A kinematic measurement method combining dynamic motion and imaging, which captures the behaviour of the hip at terminal motion, may offer improved diagnostic accuracy and enhance our understanding of the mechanics of femoroacetabular impingement (FAI). Methods: 3 embalmed cadaveric hip/pelvis specimens with implanted Roentgen Stereophotogrammetric Analysis (RSA) beads were mounted on a custom rig and imaged with a fluoroscope in four poses to simulate a clinical impingement examination: in hip extension and in three positions: near impingement, early impingement and late impingement while simulating a flexion/adduction/internal rotation manoeuvre. Hip joint kinematics were measured using 2 methods and compared: RSA (gold standard) and a custom 3-dimensional to 2-dimensional (3D–2D) image registration method which matches 3D models developed from CT to 2D fluoroscopic images. Results: Using RSA as the gold standard, bias and precision of hip joint rotations measured using 3D–2D registration demonstrated maximums of 1.64° and 3.96°, respectively. However, if the single outlier was removed, bias and precision were 0.55° and 1.38°. Bias and precision of translations had maximums of 0.51 mm and 0.77 mm, respectively. Conclusions: This 3D to 2D registration method may offer a clinically useful solution for dynamic assessment of hip impingement. If 5-mm translation and 10° of rotation represent a clinically significant difference in hip kinematics, the method’s accuracy of approximately 1 mm displacement and 1° rotation should enable detection of significant clinical differences.

scholarly journals Existence of a Neutral-Impact Maxillo-Mandibular Displacement on Upper Airways Morphology

2021 ◽  
Vol 11 (3) ◽  
pp. 177
Author(s):  
Giovanni Badiali ◽  
Ottavia Lunari ◽  
Mirko Bevini ◽  
Barbara Bortolani ◽  
Laura Cercenelli ◽  
...  
Keyword(s):  
Scientific Evidence ◽  
Three Dimensional ◽  
3D Models ◽  
Individual Case ◽  
Mandibular Advancement ◽  
Upper Airways ◽  
Displacement Threshold ◽  
Significant Difference ◽  
The Individual ◽  
Area And Volume

Current scientific evidence on how orthognathic surgery affects the airways morphology remains contradictory. The aim of this study is to investigate the existence and extension of a neutral-impact interval of bony segments displacement on the upper airways morphology. Its upper boundary would behave as a skeletal displacement threshold differentiating minor and major jaw repositioning, with impact on the planning of the individual case. Pre- and post-operative cone beam computed tomographies (CBCTs) of 45 patients who underwent maxillo-mandibular advancement or maxillary advancement/mandibular setback were analysed by means of a semi-automated three-dimensional (3D) method; 3D models of skull and airways were produced, the latter divided into the three pharyngeal subregions. The correlation between skeletal displacement, stacked surface area and volume was investigated. The displacement threshold was identified by setting three ∆Area percentage variations. No significant difference in area and volume emerged from the comparison of the two surgical procedures with bone repositioning below the threshold (approximated to +5 mm). A threshold ranging from +4.8 to +7 mm was identified, varying in relation to the three ∆Area percentages considered. The ∆Area increased linearly above the threshold, while showing no consistency in the interval ranging from −5 mm to +5 mm.

scholarly journals REALDIST: Real-valued protein distance prediction

2020 ◽  
Author(s):  
Badri Adhikari
Keyword(s):  
Structure Prediction ◽  
3D Structure ◽  
Three Dimensional ◽  
Prediction Method ◽  
3D Models ◽  
Classification Problem ◽  
Intermediate Step ◽  
Protein Distance ◽  
Multi Class Classification ◽  
Distance Prediction

AbstractProtein structure prediction continues to stand as an unsolved problem in bioinformatics and biomedicine. Deep learning algorithms and the availability of metagenomic sequences have led to the development of new approaches to predict inter-residue distances—the key intermediate step. Different from the recently successful methods which frame the problem as a multi-class classification problem, this article introduces a real-valued distance prediction method REALDIST. Using a representative set of 43 thousand protein chains, a variant of deep ResNet is trained to predict real-valued distance maps. The contacts derived from the real-valued distance maps predicted by this method, on the most difficult CASP13 free-modeling protein datasets, demonstrate a long-range top-L precision of 52%, which is 17% higher than the top CASP13 predictor Raptor-X and slightly higher than the more recent trRosetta method. Similar improvements are observed on the CAMEO ‘hard’ and ‘very hard’ datasets. Three-dimensional (3D) structure prediction guided by real-valued distances reveals that for short proteins the mean accuracy of the 3D models is slightly higher than the top human predictor AlphaFold and server predictor Quark in the CASP13 competition.

scholarly journals Three-Dimensional-Printed Model-Assisted Management of Craniovertebral Junction Abnormalities: An Institutional Experience with Literature Review

2020 ◽  
Vol 14 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Prashant Agarwal ◽  
Sanjeev Chopra ◽  
Virendra Deo Sinha ◽  
Rashim Kataria
Keyword(s):  
Vertebral Artery ◽  
Three Dimensional ◽  
3D Models ◽  
Craniovertebral Junction ◽  
Japanese Orthopedic Association ◽  
Management Protocol ◽  
Irreducible Atlantoaxial Dislocation ◽  
3D Printed ◽  
Ct Data ◽  
Assisted Management

Study Design: Prospective study.Purpose: To evaluate the utility and limitations of using three-dimensional (3D)-printed models for the management of craniovertebral (CV) junction abnormalities.Overview of Literature: In comparison to other bony and vascular anomalies, CV junction abnormalities are difficult to treat. For cases of irreducible atlantoaxial dislocation (AAD), posterior reduction and stabilization have replaced anterior decompression as the standard management protocol. The use of 3D models, such as those described herein, can provide surgeons with in-depth knowledge of the vertebral artery course and bony anomalies associated with CV junction abnormalities.Methods: Clinical and radiological features of 18 patients with CV junction abnormalities were analyzed between March 2017 and February 2019 at Sawai Man Singh Medical College, Jaipur, India. Dynamic computed tomography (CT) of the CV junction and CT angiographies of the neck with respect to the vertebral artery course at the C1–C2 joints were obtained and studied. Customized 3D models of the CV junction were then made based on the CT data, and rehearsal of the surgical procedure was performed using the 3D model one day prior to performing the actual procedure.Results: Seventeen patients had congenital-type AAD, whereas one patient had posttraumatic AAD. Improvements in neck pain and myelopathy were seen in all patients at the follow-up, as analyzed using the Visual Analog Scale and the Japanese Orthopedic Association Scale score, respectively. There were no cases of malpositioning of screws or any direct vertebral artery injury, although in one patient, the distal flow in the dominant vertebral artery was cut off as it got compressed between the bony arch and the screw head.Conclusions: Compared to computer-generated images, 3D-printed models are a more practical approach for dealing with complex CV junction abnormalities. They provide surgeons with deep insights into the complex bony anomalies as well as variations in the vertebral artery courses, thereby improving surgical outcomes.

scholarly journals Identification and “in silico” Structural Analysis of the Glutamine-rich Protein Qrp (YheA) in Staphylococcus Aureus

2019 ◽  
Vol 12 (1) ◽  
pp. 18-29
Author(s):  
Javier Escobar-Perez ◽  
Katterine Ospina-Garcia ◽  
Zayda Lorena Corredor Rozo ◽  
Ricaurte Alejandro Marquez-Ortiz ◽  
Jaime E Castellanos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Structural Analysis ◽  
Protein Function ◽  
In Silico ◽  
3D Structure ◽  
Three Dimensional ◽  
3D Models ◽  
Protein Domain ◽  
Dimensional Structure ◽  
Biofilm Growth

Background: YlbF and YmcA are two essential proteins for the formation of biofilm, sporulation, and competence in Bacillus subtilis. In these two proteins, a new protein domain called com_ylbF was recently discovered, but its role and protein function has not yet been established. Objective: In this study, we identified and performed an “in silico” structural analysis of the YheA protein, another com_ylbF-containing protein, in the opportunistic pathogen Staphylococcus aureus. Methods: The search of the yheA gene was performed using BLAST-P and tBLASn algorithms. The three-dimensional (3D) models of YheA, as well as YlbF and YmcA proteins, were built using the I-TASSER and Quark programs. The identification of the native YheA in Staphylococcus aureus was carried out through chromatography using the FPLC system. Results: We found that YheA protein is more widely distributed in Gram-positive bacteria than YlbF and YmcA. Two new and important characteristics for YheA and other com_ylbF-containing proteins were found: a highly conserved 3D structure and the presence of a putative conserved motif located in the central region of the domain, which could be involved in its function. Additionally, we established that Staphylococcus aureus expresses YheA protein in both planktonic growth and biofilm. Finally, we suggest renaming YheA as glutamine-rich protein (Qrp) in S. aureus. Conclusion: The Grp (YheA), YlbF, and YmcA proteins adopt a highly conserved three-dimensional structure, harboring a protein-specific putative motif within the com_ylbF domain, which possibly favors the interaction with their substrates. Finally, Staphylococcus aureus expresses the Grp (YheA) protein in both planktonic and biofilm growth.

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