scholarly journals Adenovirus-36 Seropositivity and Its Relation with Obesity and Metabolic Profile in Children

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Isela Parra-Rojas ◽  
Oscar Del Moral-Hernández ◽  
Aralia B. Salgado-Bernabé ◽  
Iris P. Guzmán-Guzmán ◽  
Lorenzo Salgado-Goytia ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Metabolic Profile ◽  
Density Lipoprotein ◽  
Human Adenovirus ◽  
Skinfold Thickness ◽  
Normal Weight ◽  
High Density ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Adenovirus 36

The human adenovirus 36 (Ad-36) is causally and correlatively associated in animals and humans, respectively, with increased adiposity and altered metabolic profile. In previous studies, the relationship between Ad-36 seropositivity with obesity was established in adults and children. We evaluated the association of positive antibodies to Ad-36 with obesity and metabolic profile in Mexican children. Seventy-five children with normal-weight and 82 with obesity were studied in this research. All children had a clinic assessment which included weight, height, body circumferences, and skinfold thickness. Laboratory analyzes included triglycerides, total cholesterol, high-density lipoprotein, low-density lipoprotein, and glucose and insulin levels. An enzyme-linked immunosorbent assay (ELISA) was used to determine the antibodies to Ad-36 in the serum samples. The overall Ad-36 seroprevalence was 73.9%. Ad-36 seropositivity had a higher prevalence in obese children than in normal weight group (58.6 versus 41.4%,P=0.007). Ad-36 seropositivity was associated with obesity (OR=2.66,P=0.01) and high-density lipoprotein <40 mg/dL (OR=2.85,P=0.03). The Ad-36 seropositive group had greater risk of 4 metabolic abnormalities compared with those children without none alteration. In summary, Ad-36 seropositivity was associated with obesity and low HDL-c levels in the sample of children studied.

Comparison of the Du Pont aca and Dow methods for determination of high-density lipoprotein cholesterol.

1984 ◽  
Vol 30 (1) ◽  
pp. 127-129 ◽  
Author(s):  
N N Rehak ◽  
R J Elin ◽  
R Chesler ◽  
E Johnson
Keyword(s):  
High Density Lipoprotein ◽  
Disease Control ◽  
Total Cholesterol ◽  
High Density Lipoprotein Cholesterol ◽  
Dextran Sulfate ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Serum Samples

Abstract We compared the Du Pont aca (phosphotungstate-enzymic cholesterol) and the Dow (dextran sulfate/Mg2+-enzymic cholesterol) methods for the determination of high-density lipoprotein cholesterol (HDLC) and total cholesterol in serum from 113 patients. The aca results for both total cholesterol and HDLC were significantly greater (p less than 0.0001) than the Dow results, the aca method overestimating the HDLC concentration (mean recovery 107.2% in serum samples with values assigned by the Centers for Disease Control). The precision of the aca method for HDLC was essentially the same as that of the Dow method. Bilirubin (up to 0.17 g/L), hemoglobin (up to 4 g/L), and slight lipemia (triglycerides up to 5.4 g/L) did not interfere with the aca method.

scholarly journals Increased Oxidized High-Density Lipoprotein/High-Density Lipoprotein–Cholesterol Ratio as a Potential Indicator of Disturbed Metabolic Health in Overweight and Obese Individuals

2019 ◽  
Vol 51 (1) ◽  
pp. 24-33
Author(s):  
Jelena M Janac ◽  
Aleksandra Zeljkovic ◽  
Zorana D Jelic-Ivanovic ◽  
Vesna S Dimitrijevic-Sreckovic ◽  
Jelena Vekic ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Metabolic Health ◽  
High Density ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cholesterol Ratio ◽  
Amyloid A ◽  
Potential Indicator ◽  
Metabolically Healthy

AbstractBackgroundWe evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects.MethodsThe study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis.ResultsAn independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes.ConclusionsIncreased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.

scholarly journals Variation in the Association of Body Types to Decreased High-Density Lipoprotein in Older Adults (NHANES 2005-2014)

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 213-213
Author(s):  
Queendaleen Chukwurah
Keyword(s):  
Older Adults ◽  
Body Mass Index ◽  
Waist Circumference ◽  
High Density Lipoprotein ◽  
Body Mass ◽  
Disease Risk ◽  
Density Lipoprotein ◽  
Normal Weight ◽  
High Density ◽  
Sample Population

Abstract General obesity and central obesity represent cardiovascular disease risk factors and are known to be related to dyslipidemia. I examine the variation in the association of combined body mass index/waist circumference classification to decreased high-density lipoprotein cholesterol (HDL-C). Body mass index /waist circumference (WC) cut off values were used to create six body types: normal weight with normal WC (NWT-NWC), overweight with normal WC (OWT-NWC), obese with normal WC (O-NWC), normal weight with high WC (NWT-HWC), overweight with high WC (OWT-HWC), and obese with high WC (O-HWC). HDL-C was defined as decreased if &lt; 40 mg/dl for men or &lt; 50 mg/dl for women and normal if ≥ 40 mg/dL for men or ≥ 50 mg/dL for women. Sample population included 5,772 participants of the National Health and Nutrition Examination Survey (NHANES 2005-2014) aged 50 years and older. The mean (SD) age was 61.8 (0.2), and 50.5% were females, while 10% were minority. The prevalence of decreased HDL-C was 29.1%. Analysis involved weighted multivariable logistic regression adjusted for age, race-ethnicity, gender, education, poverty-income-ratio, smoking, and alcohol intake. Regression reveals a higher likelihood of decreased HDL-C for OWT-NWC (aOR 2.12 95% CI 1.43,3.15 ), NWT-HWC (aOR 2.57 95% CI 1.59,4.16 ), OWT-HWC(aOR 3.09 95% CI 2.29,4.15 ), and O-HWC (aOR 5.30 95% CI 4.01,6.86 ) when compared to NWT-NWC. These associations are important to public health practice and policies as it demonstrates the implications of the parallel use of anthropometric measures for all body weights in health-risk assessments of older adults.

Use of a dual-precipitation procedure for measuring high-density lipoprotein 3 (HDL3) in normolipidemic serum.

1989 ◽  
Vol 35 (7) ◽  
pp. 1390-1393 ◽  
Author(s):  
T A Cloey ◽  
P S Bachorik
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Cholesterol Concentration ◽  
Precipitation Method ◽  
Serum Samples ◽  
Precipitation Procedure ◽  
The Difference ◽  
Hdl2 Cholesterol

Abstract We compared results by a dual-precipitation method (J Lipid Res 1982;23:1206-23) for measuring high-density lipoprotein 3 (HDL3) cholesterol with those by ultracentrifugation at d 1.125, using 56 fresh and 105 frozen-stored serum samples. For both methods, HDL2-cholesterol was calculated as the difference between total HDL-cholesterol and HDL3-cholesterol. In general, for pooled serum samples, agreement was closest with ultracentrifugation when we used a dextran sulfate concentration of 5.0 mg/L to precipitate the HDL2-rich fraction, although the optimal concentration varied from 3.0 to 6.8 mg/L for different pools. For individual samples, the values for HDL3 by dual precipitation averaged 12.8% lower than by ultracentrifugation. The coefficients of correlation between the two methods were HDL3, r = 0.70; and HDL2, r = 0.92. The dual-precipitation method reflected the expected sex-related differences in HDL2-cholesterol concentration and inverse relationship with triglyceride concentration.

Hemoglobin A1 correlates with the ratio of low- to high- density-lipoprotein cholesterol in normal weight type II diabetics

Metabolism ◽  
1982 ◽  
Vol 31 (11) ◽  
pp. 1084-1089 ◽  
Author(s):  
James K. Schmitt ◽  
Joel R. Poole ◽  
Stephen B. Lewis ◽  
Virgie G. Shore ◽  
Arie Maman ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Normal Weight ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Type Ii ◽  
Type Ii Diabetics ◽  
Hemoglobin A1
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