scholarly journals Development and Clinical Application of an Enzyme-Linked Immunosorbent Assay for Oxidized High-Density Lipoprotein

2020 ◽  
Author(s):  
Takeshi Okada ◽  
Mizuki Sumida ◽  
Tohru Ohama ◽  
Yuki Katayama ◽  
Ayami Saga ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Clinical Application ◽  
Immunosorbent Assay ◽  
Density Lipoprotein ◽  
High Density ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Increased Oxidized High-Density Lipoprotein/High-Density Lipoprotein–Cholesterol Ratio as a Potential Indicator of Disturbed Metabolic Health in Overweight and Obese Individuals

2019 ◽  
Vol 51 (1) ◽  
pp. 24-33
Author(s):  
Jelena M Janac ◽  
Aleksandra Zeljkovic ◽  
Zorana D Jelic-Ivanovic ◽  
Vesna S Dimitrijevic-Sreckovic ◽  
Jelena Vekic ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Metabolic Health ◽  
High Density ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cholesterol Ratio ◽  
Amyloid A ◽  
Potential Indicator ◽  
Metabolically Healthy

AbstractBackgroundWe evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects.MethodsThe study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis.ResultsAn independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes.ConclusionsIncreased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.

scholarly journals Adenovirus-36 Seropositivity and Its Relation with Obesity and Metabolic Profile in Children

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Isela Parra-Rojas ◽  
Oscar Del Moral-Hernández ◽  
Aralia B. Salgado-Bernabé ◽  
Iris P. Guzmán-Guzmán ◽  
Lorenzo Salgado-Goytia ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Metabolic Profile ◽  
Density Lipoprotein ◽  
Human Adenovirus ◽  
Skinfold Thickness ◽  
Normal Weight ◽  
High Density ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Adenovirus 36

The human adenovirus 36 (Ad-36) is causally and correlatively associated in animals and humans, respectively, with increased adiposity and altered metabolic profile. In previous studies, the relationship between Ad-36 seropositivity with obesity was established in adults and children. We evaluated the association of positive antibodies to Ad-36 with obesity and metabolic profile in Mexican children. Seventy-five children with normal-weight and 82 with obesity were studied in this research. All children had a clinic assessment which included weight, height, body circumferences, and skinfold thickness. Laboratory analyzes included triglycerides, total cholesterol, high-density lipoprotein, low-density lipoprotein, and glucose and insulin levels. An enzyme-linked immunosorbent assay (ELISA) was used to determine the antibodies to Ad-36 in the serum samples. The overall Ad-36 seroprevalence was 73.9%. Ad-36 seropositivity had a higher prevalence in obese children than in normal weight group (58.6 versus 41.4%,P=0.007). Ad-36 seropositivity was associated with obesity (OR=2.66,P=0.01) and high-density lipoprotein <40 mg/dL (OR=2.85,P=0.03). The Ad-36 seropositive group had greater risk of 4 metabolic abnormalities compared with those children without none alteration. In summary, Ad-36 seropositivity was associated with obesity and low HDL-c levels in the sample of children studied.

Triglyceride to high density lipoprotein cholesterol ratio, total cholesterol to high density lipoprotein cholesterol ratio and low ankle brachial index in an elderly population

VASA ◽  
2014 ◽  
Vol 43 (3) ◽  
pp. 189-197 ◽  
Author(s):  
Yiqiang Zhan ◽  
Jinming Yu ◽  
Rongjing Ding ◽  
Yihong Sun ◽  
Dayi Hu
Keyword(s):  
High Density Lipoprotein ◽  
Total Cholesterol ◽  
High Density Lipoprotein Cholesterol ◽  
Ankle Brachial Index ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Lipid Lowering ◽  
Cholesterol Ratio ◽  
Potential Biomarker

Background: The associations of triglyceride (TG) to high-density lipoprotein cholesterol ratio (HDL‑C) and total cholesterol (TC) to HDL‑C ratio and low ankle brachial index (ABI) were seldom investigated. Patients and methods: A population based cross-sectional survey was conducted and 2982 participants 60 years and over were recruited. TG, TC, HDL‑C, and low-density lipoprotein cholesterol (LDL-C) were assessed in all participants. Low ABI was defined as ABI ≤ 0.9 in either leg. Multiple logistic regression models were applied to study the association between TG/HDL‑C ratio, TC/HDL‑C ratio and low ABI. Results: The TG/HDL‑C ratios for those with ABI > 0.9 and ABI ≤ 0.9 were 1.28 ± 1.20 and 1.48 ± 1.13 (P < 0.0001), while the TC/HDL‑C ratios were 3.96 ± 1.09 and 4.32 ± 1.15 (P < 0.0001), respectively. After adjusting for age, gender, body mass index, obesity, current drinking, physical activity, hypertension, diabetes, lipid-lowering drugs, and cardiovascular disease history, the odds ratios (ORs) with 95 % confidence intervals (CIs) of low ABI for TG/HDL‑C ratio and TC/HDL‑C ratio were 1.10 (0.96, 1.26) and 1.34 (1.14, 1.59) in non-smokers. When TC was further adjusted, the ORs (95 % CIs) were 1.40 (0.79, 2.52) and 1.53 (1.21, 1.93) for TG/HDL‑C ratio and TC/HDL‑C ratio, respectively. Non-linear relationships were detected between TG/HDL‑C ratio and TC/HDL‑C ratio and low ABI in both smokers and non-smokers. Conclusions: TC/HDL‑C ratio was significantly associated with low ABI in non-smokers and the association was independent of TC, TG, HDL‑C, and LDL-C. TC/HDL‑C might be considered as a potential biomarker for early peripheral arterial disease screening.

Nonenzymatic glucosylation of high-density lipoprotein accelerates its catabolism in guinea pigs

Diabetes ◽  
1982 ◽  
Vol 31 (11) ◽  
pp. 1029-1032 ◽  
Author(s):  
J. L. Witztum ◽  
M. Fisher ◽  
T. Pietro ◽  
U. P. Steinbrecher ◽  
R. L. Elam
Keyword(s):  
High Density Lipoprotein ◽  
Guinea Pigs ◽  
Density Lipoprotein ◽  
High Density
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