scholarly journals Expression and Promoter Analysis of Six Heat Stress-Inducible Genes in Rice

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Wirat Rerksiri ◽  
Xianwen Zhang ◽  
Hairong Xiong ◽  
Xinbo Chen
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Cold Treatment ◽  
Evolutionary Process ◽  
High Heat ◽  
Microarray Data Analysis ◽  
Pcr Analysis ◽  
Gus Gene ◽  
Gus Gene Expression ◽  
Inducible Genes

During the long evolutionary process, plant gradually formed a series of strategies and mechanisms to cope with stress environment such as drought, heat, cold, and high salinity. Six highly heat responsive genes were identified in rice by microarray data analysis. The qRT-PCR analysis confirmed that the expression of these six genes were highly heat inducible and moderately responded to salt stress, polyethylene glycol, and abscisic acid treatment, but little affected by cold treatment. Promoters of the three highly heat-inducible genes (OsHsfB2cp, PM19p, and Hsp90p) were used to drive GUS gene expression in rice. The results of the GUS gene expression, histochemical staining, and GUS activities in panicles and flag leaves of the transgenic rice plants confirmed high heat-induced GUS activities and moderate drought-induced activities. The three promoters exhibited similar high activity lever in rice leaf under heat, but OsHsfB2cp and PM19p showed much higher activities in panicles under heat stress. Our work confirmed that the OsHsfB2c and PM19 promoters were highly heat inducible and further characterization and reconstruction ofcis-elements in their promoters could lead to the development of highly effective heat-inducible promoters for plant genetic engineering.

In Silico analysis of CCGAC and CATGTG cis-regulatory elements across genomes reveals their roles in gene regulation under stress

2021 ◽  
Vol 22 ◽  
Author(s):  
Sneha Lata Bhadouriya ◽  
Abhishek Suresh ◽  
Himanshu Gupta ◽  
Sandhya Mehrotra ◽  
Divya Gupta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Functional Analysis ◽  
Heat Stress ◽  
Statistical Analysis ◽  
In Silico Analysis ◽  
Regulatory Elements ◽  
Stress Conditions ◽  
Base Pairs ◽  
Inducible Genes

Background: Plant yield closely depends on its environment and is negatively affected by abiotic stress conditions like drought, salinity, heat, and cold. Analysis of the stress-inducible genes in Arabidopsis has previously shown that CCGAC and CATGTG play a crucial role in controlling the gene expression through the binding of DREB/CBF and NAC TFs under various stress conditions, mainly drought and salinity. Methods: The pattern of these motifs is conserved, which has been analyzed in this study to find the mechanism of gene expression through spacer specificity, inter motif distance preference, functional analysis, and statistical analysis for four different plants, namely Oryza sativa, Triticum aestivum, Arabidopsis thaliana, and Glycine max. Results: The spacer frequency analysis has shown a preference for particular spacer lengths among four genomes. The spacer specificity at all the spacer lengths which predicts dominance of particular base pairs over others, was analyzed to find the preference of the sequences in the flanking region. Functional analysis on stress-regulated genes for saline, osmotic, and heat stress clearly shows that these motif frequencies with inter motif distance (0-30) in the promoter region of Arabidopsis are highest in genes which are upregulated by saline and osmotic stress and downregulated by heat stress. Conclusion: Microarray data were analyzed to confirm the role of both motifs in stress response pathways. Transcription factors seem to prefer larger motif size with repeated CCGAC and CATGTG elements. The common preference for one spacer was further validated through Box and Whisker’s statistical analysis.

scholarly journals Selection and Validation of Appropriate Reference Genes for Real-Time Quantitative PCR Analysis in Needles of Larix olgensis under Abiotic Stresses

Forests ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 193
Author(s):  
Dandan Li ◽  
Sen Yu ◽  
Minzhen Zeng ◽  
Xiao Liu ◽  
Jia Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Real Time ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Stable Gene ◽  
Larix Olgensis ◽  
Qrt Pcr ◽  
Study Gene Expression ◽  
Selection Of

Larix olgensis Henry is an important afforestation species in northeastern China because of its fast juvenile growth, high-quality timber, and significant economic and ecological values. The selection of appropriate reference genes is necessary for the normalization of gene expression determination during quantitative real-time polymerase chain reaction (qRT-PCR) experiments. In this study, qRT-PCR was used to study gene expression. Three software packages geNorm, NormFinder, BestKeeper were used, and a comprehensive ranking of candidate reference genes was produced based on their output to evaluate the expression stability of 16 candidate reference genes from L. olgensis under drought, salt, cold, and heat stress. PP2A-1 and GAPDH ranked as the most stable reference genes under drought and cold stress, PP2A-1 and UBQ10 were most stable under salt stress, and TIP41 and ACT2 were most stable under heat stress. The least stable gene was ADP, which ranked the last under all treatments. Expression profile analysis of the antioxidant gene CAT using the two most stable and the single least stable reference genes under each stress further verified that the selected reference genes were suitable for gene expression normalization. This study provides an important foundation for the selection of suitable reference genes for the normalization and quantification of L. olgensis gene expression under abiotic stress conditions.

Studies of Cytokinin Action and Metabolism Using Tobacco Plants Expressing either the ipt or the GUS Gene Controlled by a Chalcone Synthase Promoter. IIipt and GUS Gene Expression, Cytokinin Levels and Metabolism

1997 ◽  
Vol 24 (5) ◽  
pp. 673 ◽  
Author(s):  
Jian Wang ◽  
D. S. Letham ◽  
Edwina Cornish ◽  
K. Wei ◽  
C. H. Hocart ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chalcone Synthase ◽  
Hormonal Control ◽  
Mature Leaf ◽  
Cytokinin Oxidase ◽  
Endogenous Cytokinin ◽  
Gus Gene ◽  
Ipt Gene ◽  
Cytokinin Level ◽  
Gus Gene Expression

The expression of GUS and ipt genes under control of a chalcone synthase (chs) promoter (PCHS) has been determined in tobacco (Nicotiana tabacum L.) plants and related to the development of plants expressing the chimaeric PCHS -ipt gene. GUS gene expression, which served as a model for the expression of the ipt gene, was highest in the internal phloem tissue of stems, in mature leaf laminae and in the upper part of corollas when fully open. Expression of the PCHS -ipt gene was assessed by quantifying the cytokinins produced, by determining incorporation of [3H]adenine into cytokinins and by quantifying ipt mRNA. Results from these studies were in general agreement with those based on expression of the PCHS -GUS gene. The chs promoter controlled expression of the ipt gene with some degree of tissue and temporal specificity. Expression of the ipt gene markedly elevated the cytokinin level in mature leaf laminae and the upper stems of flowering plants. The former was associated with retardation of leaf senescence and increased rates of transpiration due to changes in number, size and aperture of stomata, while the latter was associated with development of lateral shoots. In shoot tip cultures, 2-fold elevations in endogenous cytokinin level caused clear changes in development and this is discussed in relation to current concepts concerning the hormonal control of plant development. Using the transgenic tobacco tissues, it was shown that cis-zeatin is a substrate for cytokinin oxidase, that cis-zeatin is not converted to trans-zeatin in these tissues and that the endogenous cytokinin level influences the level of cytokinin oxidase activity in tissue and the rate of degradation of exogenous zeatin riboside to adenosine.

Transient GUS gene expression in Brassica napus electroporated microspores

Plant Science ◽  
1993 ◽  
Vol 93 (1-2) ◽  
pp. 177-184 ◽  
Author(s):  
Marie-Françoise Jardinaud ◽  
André Souvré ◽  
Gilbert Alibert
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Gus Gene ◽  
Gus Gene Expression
Sign in / Sign up

Export Citation Format

Share Document