scholarly journals Stimulation of TRPV1 by Green Laser Light

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Quanbao Gu ◽  
Lina Wang ◽  
Fang Huang ◽  
Wolfgang Schwarz
Keyword(s):  
Laser Irradiation ◽  
Cell Activation ◽  
Laser Light ◽  
Green Light ◽  
Medical Application ◽  
Green Laser ◽  
Ruthenium Red ◽  
Mast Cell Activation ◽  
Acupuncture Points ◽  
Stimulation Of

Low-level laser irradiation of visible light had been introduced as a medical treatment already more than 40 years ago, but its medical application still remains controversial. Laser stimulation of acupuncture points has also been introduced, and mast-cells degranulation has been suggested. Activation of TRPV ion channels may be involved in the degranulation. Here, we investigated whether TRPV1 could serve as candidate for laser-induced mast cell activation. Activation of TRPV1 by capsaicin resulted in degranulation. To investigate the effect of laser irradiation on TRPV1, we used theXenopusoocyte as expression and model system. We show that TRPV1 can functionally be expressed in the oocyte by (a) activation by capsaicin (K1/2= 1.1 μM), (b) activation by temperatures exceeding 42°C, (c) activation by reduced pH (from 7.4 to 6.2), and (d) inhibition by ruthenium red. Red (637 nm) as well as blue (406 nm) light neither affected membrane currents in oocytes nor did it modulate capsaicin-induced current. In contrast, green laser light (532 nm) produced power-dependent activation of TRPV1. In conclusion, we could show that green light is effective at the cellular level to activate TRPV1. To which extend green light is of medical relevance needs further investigation.

scholarly journals Laser Irradiation Effects at Different Wavelengths on Phenology and Yield Components of Pretreated Maize Seed

2020 ◽  
Vol 10 (3) ◽  
pp. 1189 ◽  
Author(s):  
Mohammed Hasan ◽  
Marlia Mohd Hanafiah ◽  
Ziad Aeyad Taha ◽  
Intsar H. H. AlHilfy ◽  
Mohd Nizam Mohd Said
Keyword(s):  
Laser Irradiation ◽  
Plant Height ◽  
Seed Yield ◽  
Yield Components ◽  
Laser Light ◽  
Second Harmonic ◽  
Block Design ◽  
Green Laser ◽  
Blue Laser ◽  
Growth And Yield

This study aims to compare the effects of different laser wavelengths, exposure times, and low-power-intensity laser irradiation on maize seeds. Seeds were exposed to He–Ne (632.8 nm) red laser, Nd:YAG second-harmonic-generation (532 nm) green laser, and diode (410 nm) blue laser. Four different exposure times (45, 65, 85, and 105 s) with different intensity (2 and 4 mW/cm2), for each laser were tested. Phenology and yield components (plant height, leaf area, number of rows per ear, seed yield, harvest index, yield efficiency, and grain weight) were determined. The experiment was conducted in a randomized complete block design with three replications. Plant height was found comparatively high in blue laser light—211 cm at 85 s. Blue and green laser lights showed significant increases in the number of rows per ear to 39.1 at 85 s and 45 at 65 s, respectively, compared to the control of 36 rows/ear. The order of seed yield was blue (7003.4 kg/ha) > green (6667.8 kg/ha) > red (6568.01 t/ha) based on different exposure times of 85 s, 85 s, and 105 s, respectively, compared to the control of 6.9 kg/ha. The findings indicate the possibility of using blue laser light to manipulate the growth and yield of maize.

Experimental substantiation of «green» laser light application for selective photodestruction of capillary angiodysplasia of the skin

2021 ◽  
Vol 24 (4) ◽  
pp. 43-53
Author(s):  
N. E. Gorbatova ◽  
D. A. Safin ◽  
E. N. Gasanova ◽  
A. A. Sirotkin ◽  
G. P. Kuzmin ◽  
...  
Keyword(s):  
Laser Light ◽  
Green Light ◽  
Green Laser ◽  
Regenerative Process ◽  
Vascular Pathology ◽  
In Vivo Studies ◽  
Skin Damage ◽  
Minimal Damage ◽  
Vascular Structures

Rationale. The existing techniques for treating skin capillary angiodysplasias, including «laser» photodestruction, are not effective enough and often cause undesirable thermal damage and cicatricial deformities of the skin. Thus, the effective removal of this vascular pathology is still relevant and requires further solution.Purpose. To study the nature of thermal injuries and wound regenerative processes after «green» laser irradiation with wavelength 525 nm so as to find out if it may be used for selective photodestruction of subepithelial pathological vascular structures, including angiodysplasias of skin capillaries.Material and Methods. Scallops of alive white chickens were taken as a biological model of vascular structures for in vivo studies. An experimental solid-state laser generating «green» light with wavelength 525 nm was used. This laser light has similar peaks of the absorption coefficient of hemoglobin and oxyhemoglobin which are found in skin capillaries. Features of damage and dynamics of regenerative process in the irradiated area were studied at the model of vascular structures using findings of morphological macroscopic and histological examinations.Results. Findings of the present experimental trial have inspired that selective photodestruction of subepithelial vascular structures of scallops is possible with minimal damage to the epithelium, including growth zones. In irradiated areas, one could see whitening and smoothing of the surface due to closure of vessel lumens in the subepithelial zone and formation of collagenosis layer there, as well as epithelialization of wound surface in physiological terms without any formation of cicatricial deformation of the skin.Conclusion. The selective effect of 525 nm «green» laser light at subepithelial vascular structures with minimal skin damage has been confirmed. It opens a way to apply this light for selective photodestruction of capillary angiodysplasias of the skin with good clinical and esthetic outcomes.

scholarly journals Stimulation of Hatching Efficiency in Capsulated Cysts of Artemia fransiscana Using 890 nm Diode Laser

2011 ◽  
Vol 8 (1) ◽  
pp. 66-72
Author(s):  
Baghdad Science Journal
Keyword(s):  
Energy Density ◽  
Laser Irradiation ◽  
Diode Laser ◽  
Exposure Time ◽  
Laser Light ◽  
Live Food ◽  
Optimum Dose ◽  
Hatching Percentage ◽  
Stimulation Of

Artemia fransiscana is one of the most important live food for commercial larval aquaculture. The aim of this study is to investigate the effects of 890 nm diode laser irradiation on Artemia capsulated cysts using (1-10) minutes exposure time, and 2.26x10-3 J/cm2 Fluence. The Artemia samples were obtained from two locations: Dyalaa and Basraa. After irradiation, hatching percentage (H %) and hatching efficiency(HE) of Artemia were measured after 24 and 48 hours of incubation. The results of the effect of laser light on the capsulated cysts from Dyalaa showed that the optimum dose for enhancing (H %) after 24 hours of incubation is using 10 minutes exposure time, while after 48 hours of incubation the (H %) enhancement can be achieved using 6 minutes exposure time. The optimum exposure times for (HE) enhancement after 24 and 48 hours of incubation were 5 and 7 minutes. The results of the effect of laser light on the capsulated cysts from Basraa showed that after 24 hours of incubation, the optimum exposure times for enhancement (H%) was 9 minutes, while after 48 hours of irradiation the best exposure times was 5 minutes . Very effective enhancement of (HE) was noticed after 24 hours of irradiation at 3 minutes exposure time using 2.26x10-3 J/cm2 Fluence. No enhancement was observed after 48 hours of irradiation In conclusion, 890 nm diode laser irradiation can be used successfully for increasing Hatching percentage (H %) and Hatching Efficiency (HE) of Artemia capsulated cysts using certain energy density and certain exposure times

Effects of Red and Green Laser Irradiations on the Optical Properties of an Azo-Containing Bent-Core Liquid Crystal

2010 ◽  
Vol 428-429 ◽  
pp. 588-592
Author(s):  
Yuan Ming Huang ◽  
Fu Fang Zhou ◽  
Bao Gai Zhai
Keyword(s):  
Optical Properties ◽  
Liquid Crystal ◽  
Optical Absorption ◽  
Laser Irradiation ◽  
Ethyl Alcohol ◽  
Irradiation Time ◽  
Green Light ◽  
Green Laser ◽  
Time Dependent ◽  
532 Nm

With the ultraviolet-visible spectrophotometry, we have investigated the effects of red laser (633 nm) and green laser (532 nm) irradiations on the optical absorption of the ethyl alcohol solutions of an azo-containing bent-core liquid crystal. As the irradiation duration increases, the optical absorptions at about 360 nm of the bent-core compound exhibit concentration dependent and irradiation-time dependent features. The absorptions of dilute solutions (~2.2 mg/litter) are not sensitive to the laser irradiation duration whereas the absorptions of higher concentrations (~10 mg/litter) decrease as the laser irradiation continues. Our results have demonstrated that laser irradiation induced thermolysis is responsible for the dissociation of the azo-linkages in azo-containing bent-core compound. The mechanisms on the red and green light irradiations are discussed.

scholarly journals Neuropeptide Initiated Mast Cell Activation by Transcutaneous Electrical Acupoint Stimulation of Acupoint LI4 in Rats

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Li-Zhen Chen ◽  
Yu Kan ◽  
Zhi-Yun Zhang ◽  
Yi-Li Wang ◽  
Xiao-Ning Zhang ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Acupoint Stimulation ◽  
Stimulation Of

Stimulation of the cochlea using green laser light

2009 ◽  
Author(s):  
G. I. Wenzel ◽  
S. Balster ◽  
H. H. Lim ◽  
K. Zhang ◽  
U. Reich ◽  
...  
Keyword(s):  
Laser Light ◽  
Green Laser ◽  
Stimulation Of

Lipid-rich enteral nutrition regulates mucosal mast cell activation via the vagal anti-inflammatory reflex

2013 ◽  
Vol 305 (5) ◽  
pp. G383-G391 ◽  
Author(s):  
Jacco J. de Haan ◽  
M'hamed Hadfoune ◽  
Tim Lubbers ◽  
Caroline Hodin ◽  
Kaatje Lenaerts ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Mucosal Mast Cell ◽  
Mucosal Mast Cells ◽  
Vagal Reflex ◽  
Anti Inflammatory ◽  
Mouse Mast Cell ◽  
Stimulation Of

Nutritional stimulation of the cholecystokinin-1 receptor (CCK-1R) and nicotinic acetylcholine receptor (nAChR)-mediated vagal reflex was shown to reduce inflammation and preserve intestinal integrity. Mast cells are important early effectors of the innate immune response; therefore modulation of mucosal mast cells is a potential therapeutic target to control the acute inflammatory response in the intestine. The present study investigates intestinal mast cell responsiveness upon nutritional activation of the vagal anti-inflammatory reflex during acute inflammation. Mucosal mast cell degranulation was induced in C57/Bl6 mice by administration of Salmonella enterica LPS. Lipid-rich enteral feeding prior to LPS significantly decreased circulatory levels of mouse mast cell protease at 30 min post-LPS compared with isocaloric low-lipid nutrition or fasting. CCK-1R blockage reversed the inhibitory effects of lipid-rich feeding, whereas stimulation of the peripheral CCK-1R mimicked nutritional mast cell inhibition. The effects of lipid-rich nutrition were negated by nAChR blockers chlorisondamine and α-bungarotoxin and vagal intestinal denervation. Accordingly, release of β-hexosaminidase by MC/9 mast cells following LPS or IgE-ovalbumin complexes was dose dependently inhibited by acetylcholine and nicotine. Application of GSK1345038A, a specific agonist of the nAChR α7, in bone marrow-derived mast cells from nAChR β2−/− and wild types indicated that cholinergic inhibition of mast cells is mediated by the nAChR α7 and is independent of the nAChR β2. Together, the present study reveals mucosal mast cells as a previously unknown target of the nutritional anti-inflammatory vagal reflex.

Chronic Laser Exposure Effects on Rabbit Retina

1972 ◽  
Vol 30 ◽  
pp. 54-55
Author(s):  
Burton B. Silver ◽  
Theodore Lawwill
Keyword(s):  
Laser Irradiation ◽  
Laser Light ◽  
Broad Band ◽  
Argon Laser ◽  
Atropine Sulfate ◽  
Ultrastructural Changes ◽  
Laser Exposure ◽  
Rabbit Retina ◽  
Histological Changes ◽  
Threshold Doses

Dutch-belted 1 to 2.5 kg anesthetized rabbits were exposed to either xenon or argon laser light administered in a broad band, designed to cover large areas of the retina. For laser exposure, the pupil was dilated with atropine sulfate 1% and pheny lephrine 10%. All of the laser generated power was within a band centered at 5145.0 Anstroms. Established threshold for 4 hour exposures to laser irradiation are in the order of 25-35 microwatts/cm2. Animals examined for ultrastructural changes received 4 hour threshold doses. These animals exhibited ERG, opthalmascopic, and histological changes consistent with threshold damage.One month following exposure the rabbits were killed with pentobarbitol. The eyes were immediately enucleated and dissected while bathed in 3% phosphate buffered gluteraldehyde.

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