scholarly journals EPR and FTIR Spectroscopies Study of Human Blood after Irradiation

2012 ◽  
Vol 27 ◽  
pp. 367-371 ◽  
Author(s):  
M. Polakovs ◽  
N. Mironova-Ulmane ◽  
A. Pavlenko ◽  
E. Reinholds ◽  
M. Gavare ◽  
...  
Keyword(s):  
Human Blood ◽  
Hierarchical Cluster ◽  
Time Saving ◽  
Paramagnetic Resonance ◽  
Blood Samples ◽  
Before And After ◽  
Human Blood Samples ◽  
Concentration Changes ◽  
Electron Paramagnetic

In the present work we report results of investigations of human blood before and after radioisotopeTc99mdiagnosis by electron paramagnetic resonance (EPR) and Fourier transform infrared (FTIR) spectroscopies. It is shown that EPR can detect the concentration of methaemoglobin and transferrin ions more accurately than any other technique. FTIR spectra indicated that radiation caused conformational and concentration changes of proteins. Hierarchical cluster analysis (HCA) was created as time-saving tool for discrimination of initial and irradiatedin vivohuman blood samples.

scholarly journals Protein Adductomics: Methodologies for Untargeted Screening of Adducts to Serum Albumin and Hemoglobin in Human Blood Samples

2019 ◽  
Vol 8 (1) ◽  
pp. 6 ◽  
Author(s):  
Henrik Carlsson ◽  
Stephen Rappaport ◽  
Margareta Törnqvist
Keyword(s):  
Mass Spectrometry ◽  
Serum Albumin ◽  
Human Blood ◽  
Mass Spectrometry Data ◽  
Reaction Products ◽  
Blood Samples ◽  
Large Numbers ◽  
Human Blood Samples ◽  
Critical Aspects

The reaction products of electrophiles in vivo can be measured as adducts to the abundant proteins, hemoglobin (Hb), and human serum albumin (HSA), in human blood samples. During the last decade, methods for untargeted screening of such adducts, called “adductomics”, have used liquid chromatography-mass spectrometry to detect large numbers of previously unknown Hb and HSA adducts. This review presents methodologies that were developed and used in our laboratories for Hb and HSA adductomics, respectively. We discuss critical aspects regarding choice of target protein, sample preparation, mass spectrometry, data evaluation, and strategies for identification of detected unknown adducts. With this review we give an overview of these two methodologies used for protein adductomics and the precursor electrophiles that have been elucidated from the adducts.

A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples

2020 ◽  
Vol 8 (6) ◽  
pp. 1310-1315 ◽  
Author(s):  
Manlin Fu ◽  
Wei Shen ◽  
Yajun Chen ◽  
Wenjun Yi ◽  
Chunhui Cai ◽  
...  
Keyword(s):  
Human Blood ◽  
Fluorescent Probes ◽  
Living Cells ◽  
The State ◽  
Blood Samples ◽  
Highly Sensitive ◽  
Human Blood Samples ◽  
Viscosity Changes

Intracellular viscosity can be measured to reflect the state of living cells. Fluorescent probes are powerful tools for viscosity detection in vivo.

scholarly journals Application of microRNA in Human Osteoporosis and Fragility Fracture: A Systemic Review of Literatures

2021 ◽  
Vol 22 (10) ◽  
pp. 5232
Author(s):  
Yen-Zung Wu ◽  
Hsuan-Ti Huang ◽  
Tsung-Lin Cheng ◽  
Yen-Mou Lu ◽  
Sung-Yen Lin ◽  
...  
Keyword(s):  
Human Blood ◽  
Diagnostic Value ◽  
Systemic Review ◽  
Blood Samples ◽  
In Vivo Experiments ◽  
Pubmed Database ◽  
Entry Points ◽  
Human Blood Samples

MicroRNAs (miRNAs) could serve as ideal entry points to the deregulated pathways in osteoporosis due to their relatively simple upstream and downstream relationships with other molecules in the signaling cascades. Our study aimed to give a comprehensive review of the already identified miRNAs in osteoporosis from human blood samples and provide useful information for their clinical application. A systematic literature search for relevant studies was conducted in the Pubmed database from inception to December 2020. We set two essential inclusion criteria: human blood sampling and design of controlled studies. We sorted the results of analysis on human blood samples according to the study settings and compiled the most promising miRNAs with analyzed diagnostic values. Furthermore, in vitro and in vivo evidence for the mechanisms of the identified miRNAs was also illustrated. Based on both diagnostic value and evidence of mechanism from in vitro and in vivo experiments, miR-23b-3p, miR-140-3p, miR-300, miR-155-5p, miR-208a-3p, and miR-637 were preferred candidates in diagnostic panels and as therapeutic agents. Further studies are needed to build sound foundations for the clinical usage of miRNAs in osteoporosis.

ДИНИТРОЗИЛЬНЫЕ КОМПЛЕКСЫ ЖЕЛЕЗА C ТИОЛСОДЕРЖАЩИМИ ЛИГАНДАМИ ПРЕДСТАВЛЕНЫ В ЖИВЫХ ОРГАНИЗМАХ В ОСНОВНОМ ИХ БИЯДЕРНОЙ ФОРМОЙ

2020 ◽  
Vol 65 (6) ◽  
pp. 1142-1153
Author(s):  
В.Д. Микоян ◽  
◽  
Е.Н. Бургова ◽  
Р.Р. Бородулин ◽  
А.Ф. Ванин ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Sodium Nitrite ◽  
Iron Complexes ◽  
Paramagnetic Resonance ◽  
First Case ◽  
Dinitrosyl Iron ◽  
Electron Paramagnetic ◽  
Dinitrosyl Complexes

The number of mononitrosyl iron complexes with diethyldithiocarbamate, formed in the liver of mice in vivo and in vitro after intraperitoneal injection of binuclear dinitrosyl iron complexes with N-acetyl-L-cysteine or glutathione, S-nitrosoglutathione, sodium nitrite or the vasodilating drug Isoket® was assessed by electron paramagnetic resonance (EPR). The number of the said complexes, in contrast to the complexes, formed after nitrite or Isoket administration, the level of which sharply increased after treatment of liver preparations with a strong reducing agent - dithionite, did not change in the presence of dithionite. It was concluded that, in the first case, EPR-detectable mononitrosyl iron complexes with diethyldithiocarbamate in the absence and presence of dithionite appeared as a result of the reaction of NO formed from nitrite with Fe2+-dieth- yldithiocarbamate and Fe3+-diethyldithiocarbamate complexes, respectively. In the second case, mononitrosyl iron complexes with diethyldithiocarbamate appeared as a result of the transition of iron-mononitosyl fragments from ready-made iron-dinitrosyl groups of binuclear dinitrosyl complexes, which is three to four times higher than the content of the mononuclear form of these complexes in the tissue...

scholarly journals A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16339-16350
Author(s):  
Mengkui Ding ◽  
Ling Zha ◽  
Hui Wang ◽  
Jinyao Liu ◽  
Peiwu Chen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Carcinoembryonic Antigen ◽  
Human Blood ◽  
Shell Structure ◽  
Core Shell ◽  
Label Free ◽  
Blood Samples ◽  
Human Blood Samples ◽  
Core Shell Structure ◽  
Electrochemical Immunosensing

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.

Borrelia burgdorferi Survival in Human Blood Samples

1988 ◽  
Vol 539 (1 Lyme Disease) ◽  
pp. 444-445 ◽  
Author(s):  
GUY BARANTON ◽  
ISABELLE SAINT-GIRONS
Keyword(s):  
Borrelia Burgdorferi ◽  
Human Blood ◽  
Blood Samples ◽  
Human Blood Samples

Evaluation of adriamycin nephropathy by an in vivo electron paramagnetic resonance

2005 ◽  
Vol 332 (2) ◽  
pp. 326-331 ◽  
Author(s):  
Takaaki Oteki ◽  
Sohji Nagase ◽  
Hidekatsu Yokoyama ◽  
Hiroaki Ohya ◽  
Takao Akatsuka ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Paramagnetic Resonance ◽  
Adriamycin Nephropathy ◽  
Electron Paramagnetic
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