scholarly journals Binding Studies of a New Water-Soluble Iron(III) Schiff Base Complex to DNA Using Multispectroscopic Methods

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Nahid Shahabadi ◽  
Zeinab Ghasemian ◽  
Saba Hadidi
Keyword(s):  
Schiff Base Complex ◽  
Water Soluble ◽  
Binding Modes ◽  
Binding Studies ◽  
Calf Thymus ◽  
Absorption Studies ◽  
Voltammetric Studies ◽  
Physicochemical Methods ◽  
Ct Dna ◽  
Chloride Methyl

A novel iron(III) complex [Fe(SF)](ClO4)3.2H2O; in which SF = N,N0-bis{5-[(triphenylphosphonium chloride)-methyl] salicylidene}-o-phenylenediamine) has been synthesized and characterized using different physicochemical methods. The binding of this complex with calf thymus (CT) DNA was investigated by circular dichroism, absorption studies, emission spectroscopy, voltammetric studies, and viscosity measurements. The results showed that this complex can bind to DNA via external and groove binding modes.

Interaction of a synthesized pyrene based fluorescent probe with CT-DNA: spectroscopic, thermodynamic and molecular modeling studies

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 93335-93342 ◽  
Author(s):  
Soumen Ghosh ◽  
Abdulla Al Masum ◽  
Aniruddha Ganguly ◽  
Md. Akhtarul Alam ◽  
Md. Maidul Islam ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Fluorescent Probe ◽  
Water Soluble ◽  
Calf Thymus Dna ◽  
Modeling Studies ◽  
Calf Thymus ◽  
Molecular Modeling Studies ◽  
Ct Dna

The present study demonstrates the synthesis of a new pyrene based water soluble fluorescent probe and its interaction with Calf-thymus DNA.

scholarly journals DNA binding properties, histidine interaction and cytotoxicity studies of water soluble ruthenium(ii) terpyridine complexes

2016 ◽  
Vol 45 (11) ◽  
pp. 4633-4646 ◽  
Author(s):  
Dejan Lazić ◽  
Aleksandar Arsenijević ◽  
Ralph Puchta ◽  
Živadin D. Bugarčić ◽  
Ana Rilak
Keyword(s):  
Dna Binding ◽  
Competitive Binding ◽  
Water Soluble ◽  
Binding Studies ◽  
Binding Properties ◽  
Cytotoxicity Studies ◽  
Vis Spectroscopy ◽  
Spectroscopy Studies ◽  
Ct Dna ◽  
Dna Binding Properties

UV-Vis spectroscopy studies, viscosity measurements and competitive binding studies with EB have revealed the ability of the complexes to bind to CT DNA covalently through N7 of guanine residues and non-covalently through intercalation.

Thermodynamic investigation of manganese(III) 5-(1-(4-carboxybutyl)pyridinium-4-yl) 10,15,20-tris-(1-methylpyridinium-4-yl)porphyrin with calf thymus DNA

2009 ◽  
Vol 13 (08n09) ◽  
pp. 964-972 ◽  
Author(s):  
Hamid Dezhampanah ◽  
Abdol-Khalegh Bordbar ◽  
Shahram Tangestaninejad
Keyword(s):  
Fluorescence Emission ◽  
Resonance Light Scattering ◽  
Water Soluble ◽  
Calf Thymus Dna ◽  
External Mode ◽  
Calf Thymus ◽  
Enthalpy And Entropy Changes ◽  
Enthalpy And Entropy ◽  
Hoff Equation ◽  
Ct Dna

Binding properties of two water-soluble porphyrins, manganese(III) 5-(1-(4-carboxybutyl)pyridinium-4-yl) 10,15,20-tris(1-methylpyridinium-4-yl)porphyrin ( Mn(III)5-CBPyP ) and manganese(III) 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin ( Mn(III)TMPyP ), in the presence of various concentration of calf thymus DNA (ct-DNA), has been studied in 7.5 mM phosphate buffer, pH = 7.2 and at various temperatures by UV-vis absorption, resonance light scattering (RLS) and fluorescence spectroscopy and viscosity measurement. Optical absorption and RLS measurements have demonstrated three different species of both porphyrins form in DNA solution. The thermodynamic parameters were calculated by van't Hoff equation at various temperatures. The values of -4.89 kJ.mol-1 and +65.98 J.mol-1.K-1 for Mn(III)5-CBPyP and -14.92 kJ.mol-1 and +15.46 J mol-1.K-1 for TMPyP were estimated for enthalpy and entropy changes of interaction, respectively. The data indicate that the process is exothermic and enthalpy- and entropy-driven, suggesting that electrostatic forces play a considerable role in the interaction process. The binding of both porphyins to DNA quenches fluorescence emission of ethidium bromide (EB) and the quenching process obeys linear Stern-Volmer relationship, indicating the quenching of electron transfer of EB from its binding sites by these porphyrins. The results of using these techniques indicate the external mode of binding for both porphyrins and a higher binding affinity of Mn(III)5-CBPyP with respect to Mn(III)TMPyP .

Fluorescent pyrene moiety in fluorinated C6F5-corroles increases the interaction with HSA and CT-DNA

2020 ◽  
pp. A-T
Author(s):  
Thiago V. Acunha ◽  
Otávio A. Chaves ◽  
Bernardo A. Iglesias
Keyword(s):  
Molecular Docking ◽  
Minor Groove ◽  
Spectroscopic Techniques ◽  
Binding Assays ◽  
Binding Studies ◽  
Calf Thymus ◽  
Dna Strands ◽  
Docking Calculations ◽  
Fluorescence Quenching Mechanism ◽  
Ct Dna

Two fluorinated meso-C6F5-corroles (5,15-bis(pentafluorophenyl)-10-(phenyl)corrole and 5,15-bis(pentafluorophenyl)-10-(1-pyrenyl)corrole) were biologically evaluated in terms of binding affinity to human serum albumin (HSA) and calf-thymus DNA (CT-DNA) via multiple spectroscopic techniques under physiological conditions combined with molecular docking calculations. The HSA:corrole interaction is spontaneous and moderate via static binding, disturbing both secondary and tertiary albumin structures at high fluorinated corrole concentrations. The competitive binding studies indicated positive cooperativity or allosteric activation, while molecular docking calculations suggested that both fluorinated corroles bind preferentially inside subdomains IIA and IB (sites I and III, respectively). The experimental CT-DNA binding assays indicated that fluorinated corroles interact spontaneously by non-classical modes in the minor groove of the CT-DNA strands via static fluorescence quenching mechanism. Molecular docking results also showed the minor groove as the main binding site for CT-DNA. Overall, the pyrene moiety increased the interaction with HSA and CT-DNA, which is probably due to the planarity and volume that favors the pyrene unit to be buried inside the biomacromolecule pockets.

scholarly journals DNA Binding and Antitumor Activity of α-Diimineplatinum(II) and Palladium(II) Dithiocarbamate Complexes

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Hassan Mansouri-Torshizi ◽  
Maryam Saeidifar ◽  
Fatemeh Khosravi ◽  
Adeleh Divsalar ◽  
Ali Akbar Saboury ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Fluorescence Spectra ◽  
Gel Filtration ◽  
Cell Tumor ◽  
Water Soluble ◽  
Conductivity Measurements ◽  
Low Concentrations ◽  
Calf Thymus ◽  
Elemental Analyses ◽  
Ct Dna

The two water-soluble designed platinum(II) complex, [Pt(Oct-dtc)(bpy)]NO3(Oct-dtc = Octyldithiocarbamate and bpy = 2,2′ -bipyridine) and palladium(II) complex, [Pd(Oct-dtc)(bpy)]NO3, have been synthesized and characterized by elemental analyses, molar conductivity measurements, IR,1HNMR, and electronic spectra studies. Studies of antitumor activity of these complexes against human cell tumor lines (K562) have been carried out. They show Ic50values lower than that of cisplatin. The complexes have been investigated for their interaction with calf thymus DNA (CT-DNA) by utilizing the electronic absorption spectroscopy, fluorescence spectra, and ethidium bromide displacement and gel filtration techniques. Both of these water-soluble complexes bound cooperatively and intercalatively to the CT-DNA at very low concentrations. Several binding and thermodynamic parameters are also described.

scholarly journals ComparativeIn VitroBinding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Pamita Awasthi ◽  
Nitesh Kumar ◽  
Raj Kaushal ◽  
Mohan Kumar ◽  
Shrikant Kukreti
Keyword(s):  
Circular Dichroism ◽  
Binding Constants ◽  
Blue Shift ◽  
Binding Mode ◽  
Visible Absorption ◽  
Calf Thymus ◽  
Absorption Studies ◽  
Uv Visible ◽  
Ct Dna ◽  
Circular Dichroïsm

The binding of TiCl2(dpme)2(1), (dpme = 6,6′-dimethyl-2,2′-bipyridine), Ti(ada)2(bzac)2(2), (ada = adamantylamine; bzac = benzoylacetone), and TiCl2(bzac)(bpme) (3), (bpme = 4,4′-dimethyl-2,2′-bipyrdine) with calf thymus (ct) DNA has been studied by UV-visible spectroscopy, thermal denaturation, and circular dichroism spectroscopy. In UV-visible study complexes1,2, and3showed red, blue, and red shifts, respectively, upon the addition of ct-DNA along with a significant hyperchromism. The intrinsic binding constants (Kb) calculated from UV-visible absorption studies were 2.3 × 103 M−1, 3.3 × 103 M−1and, 7.1 × 103 M−1for complexes1,2, and3, respectively. The change in melting temperature (ΔTm) was calculated to be 2-3°C for each complex. Circular dichroism (CD) study showed blue shift for complex2and red shift for complexes1and3along with rise in molecular ellipticity upon the addition of complexes. Results suggest a binding mode of complex2different than1and3.

scholarly journals IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS

1969 ◽  
Vol 130 (4) ◽  
pp. 797-808 ◽  
Author(s):  
Edward C. Franklin ◽  
Mordechai Pras
Keyword(s):  
Human Serum ◽  
Comparative Studies ◽  
Amyloid Fibrils ◽  
Complement Fixation ◽  
Cross Reactivity ◽  
Water Soluble ◽  
Antigenic Determinants ◽  
Normal Tissues ◽  
Absorption Studies ◽  
Normal Human

Eight preparations of soluble amyloid and degraded amyloid (DAM) were compared immunologically. Unlike amyloid fibrils, six of eight preparations of DAM proved to be relatively strong immunogens. Antisera to DAM reacted weakly or not at all with normal human serum or extracts of normal tissues, but were specifically reactive with amyloid fibrils or DAM. Comparative studies of DAM'S from eight different subjects showed some degree of cross-reactivity among them, yet demonstrated that they were not identical. Similar conclusions were obtained by quantitative precipitin and complement fixation analyses. Comparison of the amyloid fibrils with the homologous DAM by complement fixation and absorption studies demonstrated the existence in DAM of antigenic determinants that were lacking or inaccessible in the native fibrils. A search for amyloid precursors and antibodies to amyloid in the sera of 12 patients proved unsuccessful.

Exploring the Interaction of Ethidium Bromide and HOECHST 33258 with DNA by Means of Electrochemical Approach

2017 ◽  
Vol 12 (03) ◽  
pp. 151-161
Author(s):  
Levon A. Tavadyan ◽  
Seyran H. Minasyan ◽  
Gaspar H. Kocharyan ◽  
Ara P. Antonyan ◽  
Viktoria G. Sahakyan ◽  
...  
Keyword(s):  
Ethidium Bromide ◽  
Square Wave Voltammetry ◽  
Differential Pulse ◽  
Binding Modes ◽  
Hoechst 33258 ◽  
Wave Voltammetry ◽  
Calf Thymus ◽  
Electrochemical Approach ◽  
Pulse Voltammetry ◽  
Binding Type

The binding of both ethidium bromide (EtBr) and Hoechst 33258 (H33258) to calf thymus DNA differing by the mechanism at different ionic strengths of NaCl water solutions (2, 20 and 150[Formula: see text]mM) has been quantitatively studied by square wave voltammetry (SWV). It was revealed that EtBr binds to DNA by more than one mode: at the solution ionic strengths 2 and 150[Formula: see text]mM, two modes were revealed, at 20[Formula: see text]mM — three modes. Values of EtBr binding constant ([Formula: see text]) to DNA and the number of nucleotides per one binding site ([Formula: see text]) for respective binding modes were determined. For H33258 under the same conditions, two binding modes were found and for both these modes values of [Formula: see text] and [Formula: see text] were determined. Dependence of the binding types and parameters on the solution ionic strength by SWV method was revealed, which differs by the binding type of EtBr and H33258. The obtained data are in good accordance with the ones determined by spectroscopic (absorption and fluorescence) and differential pulse voltammetry (DPV) methods. The obtained data indicate the sensibility and high precision of SWV method which may be applied to studies on interaction of different ligands with DNA, which reveal some peculiarities of their binding to DNA that are hidden and are not found by other methods.

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