scholarly journals DNA Binding and Antitumor Activity of α-Diimineplatinum(II) and Palladium(II) Dithiocarbamate Complexes

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Hassan Mansouri-Torshizi ◽  
Maryam Saeidifar ◽  
Fatemeh Khosravi ◽  
Adeleh Divsalar ◽  
Ali Akbar Saboury ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Fluorescence Spectra ◽  
Gel Filtration ◽  
Cell Tumor ◽  
Water Soluble ◽  
Conductivity Measurements ◽  
Low Concentrations ◽  
Calf Thymus ◽  
Elemental Analyses ◽  
Ct Dna

The two water-soluble designed platinum(II) complex, [Pt(Oct-dtc)(bpy)]NO3(Oct-dtc = Octyldithiocarbamate and bpy = 2,2′ -bipyridine) and palladium(II) complex, [Pd(Oct-dtc)(bpy)]NO3, have been synthesized and characterized by elemental analyses, molar conductivity measurements, IR,1HNMR, and electronic spectra studies. Studies of antitumor activity of these complexes against human cell tumor lines (K562) have been carried out. They show Ic50values lower than that of cisplatin. The complexes have been investigated for their interaction with calf thymus DNA (CT-DNA) by utilizing the electronic absorption spectroscopy, fluorescence spectra, and ethidium bromide displacement and gel filtration techniques. Both of these water-soluble complexes bound cooperatively and intercalatively to the CT-DNA at very low concentrations. Several binding and thermodynamic parameters are also described.

Interaction of a synthesized pyrene based fluorescent probe with CT-DNA: spectroscopic, thermodynamic and molecular modeling studies

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 93335-93342 ◽  
Author(s):  
Soumen Ghosh ◽  
Abdulla Al Masum ◽  
Aniruddha Ganguly ◽  
Md. Akhtarul Alam ◽  
Md. Maidul Islam ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Fluorescent Probe ◽  
Water Soluble ◽  
Calf Thymus Dna ◽  
Modeling Studies ◽  
Calf Thymus ◽  
Molecular Modeling Studies ◽  
Ct Dna

The present study demonstrates the synthesis of a new pyrene based water soluble fluorescent probe and its interaction with Calf-thymus DNA.

scholarly journals Synthesis and Characterization of the Ligand Based on Benzimidazole and Its Copper Complex: DNA Binding and Antioxidant Activity

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Huilu Wu ◽  
Fan Kou ◽  
Fei Jia ◽  
Bin Liu ◽  
Jingkun Yuan ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Distorted Octahedral Geometry ◽  
Coordination Environment ◽  
Distorted Octahedral ◽  
Calf Thymus ◽  
Elemental Analyses ◽  
Displacement Experiments ◽  
Ct Dna

A new copper(II) complex with formulae of [Cu(buobb)2](pic)2, where buobb stands for the ligand of 1,3-bis(1- butylbenzimidazol-2-yl)-2-oxopropane and pic represents 2,4,6-trinitrophenol, has been synthesized and characterized by elemental analyses, molar conductivity, IR, UV-Vis spectra measurements, and cyclic voltammetry. The crystal structure of the copper(II) complex has been determined by X-ray single-crystal diffraction. The coordination environment around each copper(II) atom can be described as a distorted octahedral geometry. The π-π stacking interactions link the copper(II) complex into a 1D infinite network. The interactions of the ligand and the copper(II) complex with calf thymus DNA (CT-DNA) are investigated by using electronic absorption titration, ethidium bromide-DNA displacement experiments, and viscosity measurements. Additionally, the copper(II) complex’s antioxidant properties have been investigated in vitro.

scholarly journals DNA binding and photo-induced DNA cleavage activity of Elsinochrome A in visible light

2011 ◽  
Vol 26 (4-5) ◽  
pp. 289-296
Author(s):  
Fei Ma ◽  
Jing Tang ◽  
Chao Yang ◽  
Yu-Ying Feng ◽  
Shao-Hua Wei ◽  
...  
Keyword(s):  
Visible Light ◽  
Dna Cleavage ◽  
Fluorescence Spectra ◽  
Binding Mode ◽  
Strong Force ◽  
Dna Cleavage Activity ◽  
Calf Thymus ◽  
Electrostatic Binding ◽  
Ct Dna ◽  
Elsinochrome A

The interaction of Elsinochrome A (EA) with calf thymus DNA (CT-DNA) has been investigated by UV-vis spectra and fluorescence spectra. The results show that EA can bind with CT-DNA and binding sites are destroyed after irradiation by visible light, which indicates that EA is a promising candidate for photodynamic therapy. In addition, the binding mechanism is studied using fluorescence quenching test and ethidium bromide (EB) replace assay experiments. The results suggest that EA and CT-DNA are binding with a strong force and the major binding mode of EA with DNA could be the electrostatic binding.

Synthesis, Characterization and Bioactivity of Three Carboxylic Arylhydrazone Compounds

2020 ◽  
Vol 42 (1) ◽  
pp. 149-149
Author(s):  
Fengying Chen Fengying Chen ◽  
Wangting Wu Wangting Wu ◽  
Zhenguo Jin Zhenguo Jin ◽  
Shuiyang He Shuiyang He ◽  
Chengfang Qiao and Fei Yuan Chengfang Qiao and Fei Yuan
Keyword(s):  
Hydrogen Bonds ◽  
Propionic Acid ◽  
Elemental Analysis ◽  
Fluorescence Spectra ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Groove Binding ◽  
Calf Thymus ◽  
Ct Dna ◽  
Wheat Rust

Three novel carboxylic arylhydrazone compounds named 2- oxo propionic acid terephthalal acyl dihydrazone (1), 2-ketoglutaric acid terephthalal acyl dihydrazone (2) and 2-ketoglutaric acid salicyl- hydrazone (3) were prepared and characterized by elemental analysis, IR and 1H NMR. The antibacterial activities of 1 and 2 against wheat rust and coliform were investigated. The results showed that 1 had more excellent antibacterial properties than 2 against both wheat rust and coliform. In addition, the title compounds interaction with calf-thymus DNA (CT-DNA) were measured by fluorescence spectra method which indicating that they combined with CT-DNA by groove binding through hydrogen bonds.

scholarly journals Binding Studies of a New Water-Soluble Iron(III) Schiff Base Complex to DNA Using Multispectroscopic Methods

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Nahid Shahabadi ◽  
Zeinab Ghasemian ◽  
Saba Hadidi
Keyword(s):  
Schiff Base Complex ◽  
Water Soluble ◽  
Binding Modes ◽  
Binding Studies ◽  
Calf Thymus ◽  
Absorption Studies ◽  
Voltammetric Studies ◽  
Physicochemical Methods ◽  
Ct Dna ◽  
Chloride Methyl

A novel iron(III) complex [Fe(SF)](ClO4)3.2H2O; in which SF = N,N0-bis{5-[(triphenylphosphonium chloride)-methyl] salicylidene}-o-phenylenediamine) has been synthesized and characterized using different physicochemical methods. The binding of this complex with calf thymus (CT) DNA was investigated by circular dichroism, absorption studies, emission spectroscopy, voltammetric studies, and viscosity measurements. The results showed that this complex can bind to DNA via external and groove binding modes.

Thermodynamic investigation of manganese(III) 5-(1-(4-carboxybutyl)pyridinium-4-yl) 10,15,20-tris-(1-methylpyridinium-4-yl)porphyrin with calf thymus DNA

2009 ◽  
Vol 13 (08n09) ◽  
pp. 964-972 ◽  
Author(s):  
Hamid Dezhampanah ◽  
Abdol-Khalegh Bordbar ◽  
Shahram Tangestaninejad
Keyword(s):  
Fluorescence Emission ◽  
Resonance Light Scattering ◽  
Water Soluble ◽  
Calf Thymus Dna ◽  
External Mode ◽  
Calf Thymus ◽  
Enthalpy And Entropy Changes ◽  
Enthalpy And Entropy ◽  
Hoff Equation ◽  
Ct Dna

Binding properties of two water-soluble porphyrins, manganese(III) 5-(1-(4-carboxybutyl)pyridinium-4-yl) 10,15,20-tris(1-methylpyridinium-4-yl)porphyrin ( Mn(III)5-CBPyP ) and manganese(III) 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin ( Mn(III)TMPyP ), in the presence of various concentration of calf thymus DNA (ct-DNA), has been studied in 7.5 mM phosphate buffer, pH = 7.2 and at various temperatures by UV-vis absorption, resonance light scattering (RLS) and fluorescence spectroscopy and viscosity measurement. Optical absorption and RLS measurements have demonstrated three different species of both porphyrins form in DNA solution. The thermodynamic parameters were calculated by van't Hoff equation at various temperatures. The values of -4.89 kJ.mol-1 and +65.98 J.mol-1.K-1 for Mn(III)5-CBPyP and -14.92 kJ.mol-1 and +15.46 J mol-1.K-1 for TMPyP were estimated for enthalpy and entropy changes of interaction, respectively. The data indicate that the process is exothermic and enthalpy- and entropy-driven, suggesting that electrostatic forces play a considerable role in the interaction process. The binding of both porphyins to DNA quenches fluorescence emission of ethidium bromide (EB) and the quenching process obeys linear Stern-Volmer relationship, indicating the quenching of electron transfer of EB from its binding sites by these porphyrins. The results of using these techniques indicate the external mode of binding for both porphyrins and a higher binding affinity of Mn(III)5-CBPyP with respect to Mn(III)TMPyP .

scholarly journals In VitroAntitumor Activity of a Keggin Vanadium-Substituted Polyoxomolybdate and Its ctDNA Binding Properties

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Wen Qi ◽  
Ying Qin ◽  
Yanfei Qi ◽  
Li Guo ◽  
Juan Li
Keyword(s):  
Antitumor Activity ◽  
Absorption Spectra ◽  
Mtt Assay ◽  
Hela Cells ◽  
Fluorescence Spectra ◽  
Positive Control ◽  
Dependent Manner ◽  
Calf Thymus ◽  
Ir Analysis ◽  
Dose Dependent

A Keggin vanadium-substituted polyoxomolybdate, K5PMo10V2O40(PMo10V2), has been synthesized and it’s antitumor effect against Hela cells was investigated. The calf thymus DNA (ctDNA) binding ability of PMo10V2was also evaluated by UV-Vis absorption spectra and fluorescence spectra. The identity and high purity of PMo10V2was confirmed by elemental analysis and IR analysis. And the antitumor activity test of PMo10V2was carried out on Hela cancer cells line by MTT assay. The results of MTT assay show that PMo10V2significantly reduced the viability of Hela cells in a dose-dependent manner and exhibited stronger inhibitory activity against Hela cells at an IC50of 800 μg/mL, which is more effective than the positive control, 5-Fu(P<0.05). The results of the UV-Vis absorption spectra and fluorescence spectra indicated the groove or outside stacking binding between PMo10V2and ctDNA. These results show that the antitumor activity of PMo10V2may be caused by the interactions between DNA and PMo10V2.

scholarly journals Transformation of Construction Cement to a Self-Healing Hybrid Binder

2019 ◽  
Vol 20 (12) ◽  
pp. 2948 ◽  
Author(s):  
Werner E.G. Müller ◽  
Emad Tolba ◽  
Shunfeng Wang ◽  
Qiang Li ◽  
Meik Neufurth ◽  
...  
Keyword(s):  
Soil Bacteria ◽  
Construction Material ◽  
Water Soluble ◽  
The Self ◽  
Average Chain Length ◽  
Self Healing ◽  
Cement Concrete ◽  
Low Concentrations ◽  
Natural Mechanism ◽  
Long Chain

A new biomimetic strategy to im prove the self-healing properties of Portland cement is presented that is based on the application of the biogenic inorganic polymer polyphosphate (polyP), which is used as a cement admixture. The data show that synthetic linear polyp, with an average chain length of 40, as well as natural long-chain polyP isolated from soil bacteria, has the ability to support self-healing of this construction material. Furthermore, polyP, used as a water-soluble Na-salt, is subject to Na+/Ca2+ exchange by the Ca2+ from the cement, resulting in the formation of a water-rich coacervate when added to the cement surface, especially to the surface of bacteria-containing cement/concrete samples. The addition of polyP in low concentrations (<1% on weight basis for the solids) not only accelerated the hardening of cement/concrete but also the healing of microcracks present in the material. The results suggest that long-chain polyP is a promising additive that increases the self-healing capacity of cement by mimicking a bacteria-mediated natural mechanism.

Novel water soluble phosphate prodrugs of taxol® possessing in vivo antitumor activity

1993 ◽  
Vol 3 (8) ◽  
pp. 1761-1766 ◽  
Author(s):  
Yasutsugu Ueda ◽  
Amarendra B. Mikkilineni ◽  
Jay O. Knipe ◽  
William C. Rose ◽  
Anna Maria Casazza ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Water Soluble ◽  
Soluble Phosphate ◽  
In Vivo Antitumor Activity
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