scholarly journals DNA binding and photo-induced DNA cleavage activity of Elsinochrome A in visible light

2011 ◽  
Vol 26 (4-5) ◽  
pp. 289-296
Author(s):  
Fei Ma ◽  
Jing Tang ◽  
Chao Yang ◽  
Yu-Ying Feng ◽  
Shao-Hua Wei ◽  
...  
Keyword(s):  
Visible Light ◽  
Dna Cleavage ◽  
Fluorescence Spectra ◽  
Binding Mode ◽  
Strong Force ◽  
Dna Cleavage Activity ◽  
Calf Thymus ◽  
Electrostatic Binding ◽  
Ct Dna ◽  
Elsinochrome A

The interaction of Elsinochrome A (EA) with calf thymus DNA (CT-DNA) has been investigated by UV-vis spectra and fluorescence spectra. The results show that EA can bind with CT-DNA and binding sites are destroyed after irradiation by visible light, which indicates that EA is a promising candidate for photodynamic therapy. In addition, the binding mechanism is studied using fluorescence quenching test and ethidium bromide (EB) replace assay experiments. The results suggest that EA and CT-DNA are binding with a strong force and the major binding mode of EA with DNA could be the electrostatic binding.

scholarly journals Organoruthenium(II) thiosemicarbazone complexes: synthesis, spectral characterization, DNA binding and DNA cleavage studies

2013 ◽  
Vol 11 (6) ◽  
pp. 1010-1018 ◽  
Author(s):  
Gunasekaran Raja ◽  
Chinnasamy Jayabalakrishnan
Keyword(s):  
Elemental Analysis ◽  
13C Nmr ◽  
Dna Cleavage ◽  
Spectroscopic Studies ◽  
Spectral Studies ◽  
Mass Spectral ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Thiosemicarbazone Complexes ◽  
Ct Dna

AbstractA series of new ruthenium(II) complexes were synthesized with Schiff bases derived from salicylaldehyde / o-hydroxyacetophenone/ o-vanillin / 2-hydroxy-1-naphthaldehyde with thiosemicarbazide and acetyl furan. They are characterized by elemental analysis, IR, electronic, 1H NMR, 13C NMR and mass spectral studies. The elemental analysis suggests the stoichiometry to be 1:1 (metal:ligand). Four of these complexes were tested for its binding with CT-DNA using absorption spectroscopic studies and two of these complexes exhibit efficient DNA cleavage activity.

scholarly journals DNA Interaction Studies of a New Platinum(II) Complex Containing Different Aromatic Dinitrogen Ligands

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Nahid Shahabadi ◽  
Somaye Mohammadi ◽  
Robabeh Alizadeh
Keyword(s):  
Absorption Band ◽  
Bathochromic Shift ◽  
Binding Mode ◽  
Dna Interaction ◽  
Quenching Mechanism ◽  
Binding Interaction ◽  
Fluorescence Studies ◽  
Cyclic Voltametry ◽  
Calf Thymus ◽  
Ct Dna

A new mononuclear Pt(II) complex, [Pt(DMP)(DIP)]Cl2.H2O, in which DMP is 4,4-dimethyl-2,2-bipyridine and DIP is 4,7-diphenyl-1,10-phenantroline, has been synthesized and characterized by physicochemical and spectroscopic methods. The binding interaction of this complex with calf thymus DNA (CT-DNA) was investigated using fluorimetry, spectrophotometry, circular dichroism, viscosimetry and cyclic voltametry (CV). UV-VIS spectrum showed 4 nm bathochromic shift of the absorption band at 280 nm along with significant hypochromicity for the absorption band of the complex. The intrnisic binding constant is more in keeping with intercalators and suggests this binding mode. The viscosity measurements showed that the complex-DNA interaction can be hydrophobic and confirm intercalation. Moreover, the complex induced detectable changes in the CD spectrum of CT-DNA. The fluorescence studies revealed that the probable quenching mechanism of fluorescence of the complex by CT-DNA is static quenching. The thermodynamic parameters ( and ) showed that main interaction with hydrogenic forces occurred that is intercalation mode. Also, CV results confirm this mode because, with increasing the CT-DNA concentration, shift to higher potential was observed.

scholarly journals DNA Interaction and DNA Cleavage Studies of a New Platinum(II) Complex Containing Aliphatic and Aromatic Dinitrogen Ligands

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Nahid Shahabadi ◽  
Soheila Kashanian ◽  
Maryam Mahdavi ◽  
Noorkaram Sourinejad
Keyword(s):  
Dna Cleavage ◽  
Helical Structure ◽  
Dna Interaction ◽  
Van Der Waals Interactions ◽  
Double Helical Structure ◽  
Intercalative Mode ◽  
Physico Chemical ◽  
Calf Thymus ◽  
Enthalpy And Entropy Changes ◽  
Ct Dna

A new Pt(II) complex, [Pt(DIP)(LL)](NO3)2(in which DIP is 4,7-diphenyl-1,10-phenanthroline and LL is the aliphatic dinitrogen ligand,N,N-dimethyl-trimethylenediamine), was synthesized and characterized using different physico-chemical methods. The interaction of this complex with calf thymus DNA (CT-DNA) was investigated by absorption, emission, circular dichroism (CD), and viscosity measurements. The complex binds to CT-DNA in an intercalative mode. The calculated binding constant,Kb, was  M−1. The enthalpy and entropy changes of the reaction between the complex and CT-DNA showed that the van der Waals interactions and hydrogen bonds are the main forces in the interaction with CT-DNA. In addition, CD study showed that phenanthroline ligand insert between the base pair stack of double helical structure of DNA. It is remarkable that this complex has the ability to cleave the supercoiled plasmid.

scholarly journals Interaction of Duloxetine Hydrochloride with Deoxyribonucleic Acid Measured by Fluorescence Spectroscopy

2016 ◽  
Vol 14 (2) ◽  
pp. 199-206
Author(s):  
Raznin Akter Joly ◽  
Md Reazul Islam ◽  
Sonia Sultana ◽  
Asma Rahman ◽  
Md Zakir Sultan ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Deoxyribonucleic Acid ◽  
Antidepressant Drug ◽  
Living Organism ◽  
Binding Mode ◽  
Duloxetine Hydrochloride ◽  
Calf Thymus ◽  
Binding Forces ◽  
Ct Dna ◽  
Hypochromic Effect

Interactions with many clinically active therapeutic agents with DNA are well studied and it is necessary to decipher the structure of DNA and to investigate the pathological implications of those molecules in living organism. This study investigated the interaction of antidepressant drug Duloxetine-hydrochloride (DLX) with calf thymus DNA (ct-DNA). The interaction of DLX with ct-DNA was studied employing fluorescence spectroscopy. Hypochromic effect was found in the absorption spectra of duloxetine, and its wavelength had no shift in the presence of DNA indicating external binding mode of duloxetine to DNA. Fluorescence spectroscopic results showed the quenching of fluorescence intensity of DLX in presence of DNA indicating the interaction between DLX and DNA. Hydrophobic interaction and hydrogen bonding played the dominating role in DLX-DNA binding and binding forces also indicate the binding site of duloxetine to be at the minor groove of DNA.Dhaka Univ. J. Pharm. Sci. 14(2): 199-206, 2015 (December)

N-Methylpyridylporphyrin tailed with folate conjugate as a potential lysosomal-targeted photosensitizer: Synthesis, DNA interaction, singlet oxygen and subcellular localization

2019 ◽  
Vol 23 (06) ◽  
pp. 679-684
Author(s):  
Yi-Mei Zhao ◽  
Qian-Qian Lu ◽  
Si Yao ◽  
Hui-Fang Su ◽  
Hong-Jian Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Fluorescence Spectra ◽  
Binding Constants ◽  
Binding Mode ◽  
Dna Interaction ◽  
H Nmr ◽  
Calf Thymus ◽  
Folate Conjugate ◽  
Tumor Drugs

In recent years, great interest has been focused on the use of photosensitizers (PS) for photodynamic therapy (PDT) as safe and effective anti-tumor drugs. As a good lysosomal-targeted drug, folic acid (FA) is highly interesting as well. [Formula: see text]-methylpyridylporphyrin tailed with folate conjugate (Me-Por-FA) was newly designed and synthesized and the structure was confirmed by UV-vis, IR, 1H NMR, MS and elemental analysis. The interaction of this porphyrin with calf thymus DNA was the intercalative binding mode, which was confirmed by ultraviolet and fluorescence spectra, and the binding constants [Formula: see text] was 6.24 × 104 L/mol. The singlet oxygen (1O[Formula: see text] generated by Me-Por-FA was determined by 1, 3-diphenylisobenzofuran (DPBF) method using tetrapyridylporphyrin (H[Formula: see text]TMPyP) as a comparison with the following order: H2TMPyP > Me-Por-FA. Stained with LysoTracker[Formula: see text] Green DND-26, Me-Por-FA was mainly distributed over the lysosomes during 4 h, but H[Formula: see text]TMPyP was not. This suggests that Me-Por-FA could be developed as a targeted photosensitizer for precise photodynamic therapy.

Synthesis, Characterization and Bioactivity of Three Carboxylic Arylhydrazone Compounds

2020 ◽  
Vol 42 (1) ◽  
pp. 149-149
Author(s):  
Fengying Chen Fengying Chen ◽  
Wangting Wu Wangting Wu ◽  
Zhenguo Jin Zhenguo Jin ◽  
Shuiyang He Shuiyang He ◽  
Chengfang Qiao and Fei Yuan Chengfang Qiao and Fei Yuan
Keyword(s):  
Hydrogen Bonds ◽  
Propionic Acid ◽  
Elemental Analysis ◽  
Fluorescence Spectra ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Groove Binding ◽  
Calf Thymus ◽  
Ct Dna ◽  
Wheat Rust

Three novel carboxylic arylhydrazone compounds named 2- oxo propionic acid terephthalal acyl dihydrazone (1), 2-ketoglutaric acid terephthalal acyl dihydrazone (2) and 2-ketoglutaric acid salicyl- hydrazone (3) were prepared and characterized by elemental analysis, IR and 1H NMR. The antibacterial activities of 1 and 2 against wheat rust and coliform were investigated. The results showed that 1 had more excellent antibacterial properties than 2 against both wheat rust and coliform. In addition, the title compounds interaction with calf-thymus DNA (CT-DNA) were measured by fluorescence spectra method which indicating that they combined with CT-DNA by groove binding through hydrogen bonds.

Synthesis of tetra(trimethylammonio)phthalocyanato zinc tetraiodide, [ZnPc(NMe3)4]I4, and a spectrometric investigation of its interaction with calf thymus DNA

2009 ◽  
Vol 13 (12) ◽  
pp. 1255-1261 ◽  
Author(s):  
Wubiao Duan ◽  
Zhenxin Wang ◽  
Michael J. Cook
Keyword(s):  
Phosphate Buffer Solution ◽  
Binding Constants ◽  
Calf Thymus Dna ◽  
Buffer Solution ◽  
Solution Ph ◽  
Step Procedure ◽  
Calf Thymus ◽  
Electrostatic Binding ◽  
High Concentrations ◽  
Ct Dna

The phthalocyanine salt [ ZnPc(NMe3)4]I4 was synthesized from 4-nitrophthalonitrile using a three-step procedure. The interaction of [ ZnPc(NMe3)4]4+ with calf thymus DNA (CT DNA) has been investigated by UV-vis and fluorescence spectrometric methods. [ ZnPc(NMe3)4]4+ exists in a non-monomeric form, proposed to be a dimer, in phosphate buffer solution (pH 6.82). Spectral changes show that in the presence of high concentrations of CT DNA added to the solution, [ ZnPc(NMe3)4]4+ is bound in a monomeric state with evidence suggesting it is located in a DNA groove. At lower concentrations of DNA there is evidence of stacking of non-monomeric [ ZnPc(NMe3)4]4+ onto the DNA. Two intrinsic binding constants for the interaction of [ ZnPc(NMe3)4]4+ with CT DNA, 1.33 × 105 and 2.56 × 104 M-1 have been obtained. Electrostatic binding is shown to play an important role in the interaction of [ ZnPc(NMe3)4]4+ with nucleic acids.

scholarly journals Synthesis, DNA interaction and antibacterial activities of La(III) and Gd(III) complexes of Schiff base derived from o-vanillin and lysine

2009 ◽  
Vol 7 (1) ◽  
pp. 105-110 ◽  
Author(s):  
Jian-Ping Cheng ◽  
Qiu-Yue Lin ◽  
Hu Rui-Ding ◽  
Wen-Zhong Zhu ◽  
Hua-Qiong Li ◽  
...  
Keyword(s):  
Schiff Base ◽  
Binding Mode ◽  
Dna Interaction ◽  
Antibacterial Activities ◽  
Azomethine Nitrogen ◽  
Moderate Activity ◽  
Phenolic Oxygen ◽  
Calf Thymus ◽  
Ct Dna ◽  
Remarkable Reduction

AbstractTwo novel complexes, [La(HL)(H2O)2NO3] · NO3 · H2O and [Gd(HL)(H2O)2NO3] · NO3 · H2O, where HL is a Schiff base derived from o-vanillin and lysine, have been synthesized and characterized by elemental analysis, conductivity measurements, IR, 1H NMR and thermogravimetric analyses (TGA). The Schiff base ligand behaves as a tetradentate, coordinating through azomethine nitrogen, phenolic oxygen and two carboxylic oxygen atoms. The interaction of these complexes with calf thymus DNA (CT-DNA) was also investigated by spectrometric titration and viscometric measurements. The faint hypochromism of the complexes in the absorption spectra, the remarkable reduction of fluorescence intensity of ethidium bromide (EB) bound DNA, together with a small decrease in the viscosity of the DNA suggest that a partial intercalation may be the preferred binding mode between these two complexes and DNA. The antibacterial activity testing revealed that the complexes and their precursor Schiff base show a weak to moderate activity against Bacillus subtilis, Staphylococcus aureus and Escherichia coli.

scholarly journals Synthesis, characterization and DNA cleavage activity of nickel(II) adducts with aromatic heterocyclic bases

2010 ◽  
Vol 75 (1) ◽  
pp. 61-74 ◽  
Author(s):  
Surendra Babu ◽  
Pitchika Krishna ◽  
Hussain Reddy ◽  
G.H. Philip
Keyword(s):  
Dna Cleavage ◽  
Magnetic Moments ◽  
Nuclease Activity ◽  
Mixed Ligand ◽  
Mixed Ligand Complexes ◽  
Cleavage Activity ◽  
Redox Couples ◽  
Dna Cleavage Activity ◽  
Calf Thymus ◽  
Heterocyclic Bases

Mixed ligand complexes of nickel(II) with 2,4-dihydroxyacetophenone oxime (DAPO) and 2,4-dihydroxybenzophenone oxime (DBPO) as primary ligands, and pyridine (Py) and imidazole (Im) as secondary ligands were synthesized and characterized by molar conductivity, magnetic moments measurements, as well as by electronic, IR, and 1H-NMR spectroscopy. Electrochemical studies were performed by cyclic voltammetry. The active signals are assignable to the NiIII/II and NiII/I redox couples. The binding interactions between the metal complexes and calf thymus DNA were investigated by absorption and thermal denaturation. The cleavage activity of the complexes was determined using double-stranded pBR322 circular plasmid DNA by gel electrophoresis. All complexes showed increased nuclease activity in the presence of the oxidant H2O2. The nuclease activities of mixed ligand complexes were compared with those of the parent copper(II) complexes.

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