scholarly journals Analysis of Genetic Diversity in Astragalus rhizanthus Benth. ssp. rhizanthus var. rhizanthus (Fabaceae) Using Molecular Markers from India

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Kumar Kamal Anand ◽  
Ravi Kumar Srivastava ◽  
Lal Babu Chaudhary
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Diversity ◽  
Jammu And Kashmir ◽  
Indian Himalaya ◽  
Information Index ◽  
Inter Simple Sequence Repeats ◽  
Diversity Studies ◽  
The Himalaya

Astragalus rhizanthus with three infraspecific taxa (i.e., A. rhizanthus ssp. rhizanthus var. rhizanthus, A. rhizanthus ssp. rhizanthus var. pindreensis, and A. rhizanthus ssp. candolleanus) is widely scattered in the Himalaya from Jammu and Kashmir to Uttarakhand provinces in India. Among them, A. rhizanthus ssp. rhizanthus var. rhizanthus exhibits enormous morphological diversity throughout its range of distribution. An assessment of genetic diversity studies was undertaken to understand the level and pattern of diversity, using Inter simple sequence repeats (ISSR), Random amplified polymorphic DNA (RAPD) and Directed amplification of minisatellite DNA (DAMD) profiles. Fifteen ISSR, 18 RAPD and 6 DAMD primers were used to unravel the diversity among 20 genotypes collected from the known localities in the Indian Himalaya. A total of 242 bands from ISSR, 352 from RAPD and 142 from DAMD were obtained with an average of 92.23% polymorphism in the species. Pair-wise genetic similarity for the cumulative data was determined using Jaccard's similarity coefficient which varied from 0.19 to 0.84. A combined UPGMA dendrogram was generated which revealed that different genotypes exhibited their affinity according to their geographical distribution. Tree topology suggests the existence of two distinct groups of the genotypes. Gene diversity (H) and Shannon's information index (I) were estimated and these values were found higher in the genotypes collected from Jammu and Kashmir than Himachal Pradesh.

scholarly journals Genetic Dissection of New Genotypes of Drumstick Tree (Moringa oleiferaLam.) Using Random Amplified Polymorphic DNA Marker

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Shamsuddeen Rufai ◽  
M. M. Hanafi ◽  
M. Y. Rafii ◽  
S. Ahmad ◽  
I. W. Arolu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Marker ◽  
Diversity Index ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Dissection ◽  
Improvement Program ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Genetic Diversity Study

The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20Moringa oleiferawere carried out with the aid of twelve primers from, random amplified polymorphic DNA marker. The seeds of twentyM. oleiferagenotypes from various origins were collected and germinated and raised in nursery before transplanting to the field at University Agricultural Park (TPU). Genetic diversity parameter, such as Shannon's information index and expected heterozygosity, revealed the presence of high genetic divergence with value of 1.80 and 0.13 for Malaysian population and 0.30 and 0.19 for the international population, respectively. Mean of Nei's gene diversity index for the two populations was estimated to be 0.20. In addition, a dendrogram constructed, using UPGMA cluster analysis based on Nei's genetic distance, grouped the twentyM. oleiferainto five distinct clusters. The study revealed a great extent of variation which is essential for successful breeding and improvement program. From this study,M. oleiferagenotypes of wide genetic origin, such as T-01, T-06, M-01, and M-02, are recommended to be used as parent in future breeding program.

Genetic Diversity of Stephanie kwangsiensis H. S. Lo and Salvia prionitis Hance after Transplanted and Micropropagated

2012 ◽  
Vol 518-523 ◽  
pp. 5460-5467
Author(s):  
Chuan Ming Fu ◽  
Yan Qin ◽  
Feng Luan Tang ◽  
Yun Ping Shi ◽  
Zhi Guo Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Tissue Culture ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Chinese Herbs ◽  
High Genetic Diversity ◽  
Information Index ◽  
Rapd Pcr

Stephanie Kwangsiensis H. S. Lo and Salvia prionitis Hance were two rare and valuable Chinese herbs. Genetic diversity of the two plants were studied by using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers after they were transplantation and tissue culture. Results indicated that Nei’s gene diversity (H), Shannon’s information index (I), the percentage of polymorphic loci (PPL) of source / transplantation / tissue culture materials of S. Kwangsiensis H. S. Lo were H=0.282 / 0.195/ 0.056, I=0.415 / 0.290 / 0.087, PPL=75.6% / 56.1% /19.5% based on RAPDs and H=0.295 / 0.292 / 0.111, I=0.445 / 0.435 / 0.166, PPL=85.3% / 85.3% / 32.8% based ISSRs. And the same parameters of source / transplantation / tissue culture materials of S. prionitis Hance were H=0.193 / 0.192 / 0.231, I=0.291 / 0.289 /0.347, PPL=57.8% / 57.2% / 68.6% based on RAPDs and H=0.217 / 0.217 / 0.155, I=0.327 / 0.326 /0.235, and PPL=64.2% / 64.2% / 49.1% based on ISSRs instead. For the transplanted materials, genetic diversity was slightly declining with the disappearance of adversity. And for the tissue culture materials, more genets sampled for tissue culture, less reduction of genetic diversity; and fewer genets sampled, more reduction of genetic diversity. In addition, high genetic diversity appearing in tissue culture materials of S. prionitis Hance partially generate from somatic mutations. And these mutations maybe related to RAPD-PCR regions and just had been checked out by RAPDs.

Assessment of Genetic Diversity in Ethiopian Sesame (Sesamum indicum L.) Germplasm using Random Amplified Polymorphic DNA (RAPD) Markers

2015 ◽  
Vol 5 (2) ◽  
pp. 639-649
Author(s):  
Mohammed Abate Dawud ◽  
Firew Mekbib Alemu
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Sesamum Indicum ◽  
Dissimilarity Matrix ◽  
Information Index ◽  
Sesame Genotypes ◽  
High Level ◽  
Maximum Similarity

Genetic diversity among 128 sesame (Sesamum indicum L.) genotypes representing 10 geographically distinct populations in Ethiopia was assessed at DNA level using RAPD analysis. Eleven RAPD primers used amplified a total of 149 bands, of which 142 (95. 45%) were polymorphic. Each primer generated 7 to 23 amplified fragments with an average of 13.5 bands per primer. Percent of polymorphic loci (P%), number of different (Na) and effective (Ne) alleles along with Shannon information index (I) and Nei’s gene diversity (He) values suggested that the population of Oromia was the most diverse of all populations, while populations from Afar (cultivars) and AM-NSh were found to be the least diverse. Based on average dissimilarity values obtained with RAPD primers, AM-NG-25, SNNP-7 and SNNP-8 were the most distinct of all genotypes, while genotypes ORO-20 and TIGR-5 showed maximum similarity with others. The UPGMA clustering based on the dissimilarity matrix clustered the genotypes into 3 major groups and 11 subgroups, while three genotypes viz., BENSH-6, ORO-14 and SNNP-5 were found out-grouped from the rest and did not join any of the cluster; they are then most divergent genotypes. Generally, both clustering and PCoA patterns revealed that most genotypes located geographically far apart were found to cluster in the same group, while those genotypes from the same origin dispersed. Overall results indicated that RAPD technique revealed a high level of genetic variation among sesame genotypes collected from diverse ecologies of Ethiopia. 

Genetic Divergence among Populations of Invasive Alien Species Lantana camara L. from Selected Areas of Bangladesh

2021 ◽  
Vol 1 (01) ◽  
pp. 9-14
Author(s):  
SAILA KABIR ◽  
MD ABUL KASHEM ◽  
MOHAMMAD ZABED HOSSAIN
Keyword(s):  
Genetic Diversity ◽  
Alien Species ◽  
Invasive Alien Species ◽  
Gene Diversity ◽  
Lantana Camara ◽  
Native Plant ◽  
High Genetic Diversity ◽  
Native Plant Species ◽  
Information Index ◽  
Genetic Clustering

Lantana camara L., a well-known invasive alien species causing invasion and posing threat to native plant species community in different regions of Bangladesh. The present study aimed to investigate the genetic diversity of L. camara populations in different regions of Bangladesh. Eight RAPD markers were used in order to probe into its genetic variability. Total number of bands (202), polymorphic loci (104), per-centage of polymorphism (97.20%), average Shanon’s information index (0.3051±0.115), Nei’s gene diversity (0.4733±0.144) was found and in different populations and multiple divergent genetic clustering along with presence of unique alleles (4) for RAPD revealed high genetic diversity among the populations of L. camara in different regions of Bangladesh.

ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

2021 ◽  
Author(s):  
Lalit Arya ◽  
Ramya Kossery Narayanan ◽  
Anjali Kak ◽  
Chitra Devi Pandey ◽  
Manjusha Verma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Morinda Citrifolia ◽  
Species Differentiation ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

scholarly journals Assessment of Genetic Diversity and Population Structure of Some Soft and Hard Wheat Varieties Based on SSR Marker

2020 ◽  
Vol 8 (3) ◽  
pp. 80-87
Author(s):  
Yousif M. Fattah ◽  
Nergiz N. Tayib
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Triticum Aestivum L ◽  
Wheat Varieties ◽  
Discriminating Power ◽  
Triticum Spp ◽  
Cultivated Species ◽  
The Common ◽  
Diversity Studies

Wheat (Triticum spp.) is one of the most important cereal crops in Iraq and the world. It includes many species and varieties.  The two major cultivated species of wheat are, durum wheat (Tritium durum Desf.) which is tetraploid (2n= 28) and the common wheat (Triticum aestivum L.) which is hexaploid (2n = 42). Ten wheat varieties from both species were examined using ten Simple   sequence repeat (SSR) markers (WMC17, WMC20, WMC21, WMC24, WMC25, WMC48, WMC50, WMC283, Xgwm11 and Xgwm626). Various genetic parameters were calculated using Power Marker V3.25 software. A total of 156 alleles were detected in both species. The gene diversity in wheat varieties from both species collectively varied from 0.85 to 1.00, which indicates considerable genetic diversity in the examined varieties. All markers used in this study were highly informative and the polymorphic information content (PIC) values were higher than 0.50 in all loci. Hence all markers are considered useful for genetic diversity studies in wheat’s populations. The dendrogram separated the populations into two main clades and many subgroups. Azadi variety was simplicifolious. This study confirms the discriminating power of SSR typing and its usefulness for comparison within hard and soft wheat populations. 

scholarly journals Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of EndangeredPolyporus umbellatus

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Yuejin Zhang ◽  
Yuanyuan Chen ◽  
Ruihong Wang ◽  
Ailin Zeng ◽  
Michael K. Deyholos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Expressed Sequence Tags ◽  
Large Scale ◽  
Gene Diversity ◽  
High Genetic Diversity ◽  
Information Index ◽  
Polyporus Umbellatus ◽  
Ssr Primers ◽  
Expressed Sequence

A large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications. The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively. Mononucleotide was the most abundant type, followed by trinucleotide. Among five families, Ganodermataceae occupied the most SSR markers, followed by Coriolaceae. Functional prediction of SSR marker-containing EST sequences inGanoderma lucidumobtained three main groups, namely, cellular component, biological process, and molecular function. Thirty EST-SSR primers were designed to evaluate the genetic diversity of 13 naturalPolyporus umbellatusaccessions. Twenty one EST-SSRs were polymorphic with average PIC value of 0.33 and transferability rate of 71%. These 13P.umbellatusaccessions showed relatively high genetic diversity. The expected heterozygosity, Nei’s gene diversity, and Shannon information index were 0.41, 0.39, and 0.57, respectively. Both UPGMA dendrogram and principal coordinate analysis (PCA) showed the same cluster result that divided the 13 accessions into three or four groups.

scholarly journals Genetic Differentiation of Jack Pine (Pinus banksiana) and Red Pine (P. resinosa) Populations From Metal Contaminated Areas in Northern Ontario (Canada) Using ISSR Markers

2008 ◽  
Vol 57 (1-6) ◽  
pp. 333-340 ◽  
Author(s):  
M. Ranger ◽  
K. K. Nkongolo ◽  
P. Michael ◽  
P. Beckett
Keyword(s):  
Genetic Diversity ◽  
Pinus Banksiana ◽  
Metal Accumulation ◽  
Gene Diversity ◽  
Jack Pine ◽  
Issr Markers ◽  
Red Pine ◽  
Northern Ontario ◽  
Polymorphic Loci ◽  
Information Index

Abstract Metal accumulation in soil and plant tissues has caused severe ecological damage in forest ecosystems in the Sudbury region. The main objective of the present study was to determine the levels of genetic diversity in jack and red pine populations growing in metal contaminated and uncontaminated areas. Newly introduced populations were compared to 40 to 60 old populations. For jack pine, the percentage of polymorphic loci (P %) ranged from 14.6% to 45.8% with a mean of 31.6%. Nei’s gene diversity (h) varied from 0.046 to 0.169 with an average of 0.100, and Shannon’s index (I) ranged from 0.070 to 0.250 with an average of 0.153. The level of genetic variation was much lower in the red pine populations. For this species, the level of polymorphic loci varied from 4.55% to 27.27%. The mean for Nei’s gene diversity and Shannon’s information index, were 0.034 and 0.053, respectively. The highest genetic diversity values were observed in new plantations being developed by the Sudbury reforestation program. Overall, the genetic distance among the Pinus banksiana populations revealed that all the populations analyzed were genetically close to each other. There was no association between metal accumulation and genetic diversity for both species.

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