Efficiency of random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers for genotype fingerprinting and genetic diversity studies in canola (Brassica napus)

2012 ◽  
Vol 11 (24) ◽  
Author(s):  
Hala M. Abdelmigid
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Simple Sequence Repeats ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Inter Simple Sequence Repeats ◽  
Diversity Studies ◽  
Simple Sequence

scholarly journals Analysis of Genetic Diversity Between Eriobotrya japonica (Thunb.) Lindl. (Rosaceae) Populations Growing in the Aegean Region of Turkey

2020 ◽  
Vol 77 (2) ◽  
pp. 71
Author(s):  
Emre SEVİNDİK ◽  
Kemalcan OKAN ◽  
Fadime EFE
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Simple Sequence Repeats ◽  
Random Amplified Polymorphic Dna ◽  
Eriobotrya Japonica ◽  
Analysis Program ◽  
Aegean Region ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

In this study, we performed a genetic diversity using RAPD (Random amplified polymorphic DNA) and ISSR (Inter-simple sequence repeats) markers for some Eriobotrya japonica populations grown in the Aegean region of Turkey. PCR was performed with all DNA samples and primers with ability of scoring band. Phylogenetic analysis and pairwise genetic distance between the populations were calculated by using the PAUP analysis program. According to the RAPD data, a total of 33 bands were obtained and 21 of them were polymorphic. The closest genetic distance (0.09677) was found between Aydın and Nazilli populations, while the most distant value was found 0.44000 between Salihli and Nazilli populations. According to the ISSR data, a total of 43 bands were obtained and 18 of them were polymorphic. The closest genetic distance (0.09302) was found between İzmir and Aydın populations, while the most distant values (0.48276) were found between Denizli and Nazilli populations. As a result of this study, the rate of polymorphism obtained in ISSR technique was found to be lower than the RAPD technique.

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