scholarly journals Bone Marrow Derivation of Interstitial Cells of Cajal in Small Intestine Following Intestinal Injury

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Dengqun Liu ◽  
Fengchao Wang ◽  
Zhongmin Zou ◽  
Shiwu Dong ◽  
Junping Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Myenteric Plexus ◽  
Interstitial Cells Of Cajal ◽  
Mononuclear Cells ◽  
Interstitial Cells ◽  
Positive Staining ◽  
Alternative Source ◽  
Cells Of Cajal

Interstitial cells of Cajal (ICCs) in gastrointestinal tract are specialized cells serving as pacemaker cells. The origin of ICCs is currently not fully characterized. In this work, we aimed to study whether bone marrow-derived cells (BMDCs) could contribute to the origin of ICCs in the muscular plexus of small intestine using GFP-C57BL/6 chimeric mice.Engraftment of BMDCs in the intestine was investigated for GFP expression. GFP positive bone marrow mononuclear cells reached a proportion of95.65%±3.72%at different times in chimerism. Donor-derived cells distributed widely in all the layers of the gastrointestinal tract. There were GFP positive BMDCs in the myenteric plexus, which resembled characteristics of ICCs, including myenteric location, c-Kit positive staining, and ramified morphology. Donor-derived ICCs in the myenteric plexus contributed to a percentage ranging9.25%±4.9%of all the ICCs in the myenteric plexus. In conclusion, here we described that donor-derived BMDCs might differentiate into gastrointestinal ICCs after radiation injury, which provided an alternative source for the origin of the ICCs in the muscular plexus of adult intestine. These results further identified the plasticity of BMDCs and indicated therapeutic implications of BMDCs for the gastrointestinal dysmotility caused by ICCs disorders.

Impaired development of interstitial cells and intestinal electrical rhythmicity in steel mutants

1995 ◽  
Vol 269 (6) ◽  
pp. C1577-C1585 ◽  
Author(s):  
S. M. Ward ◽  
A. J. Burns ◽  
S. Torihashi ◽  
S. C. Harney ◽  
K. M. Sanders
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Tyrosine Kinase ◽  
Myenteric Plexus ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Slow Waves ◽  
Tyrosine Kinase Receptor ◽  
Steel Factor ◽  
Cells Of Cajal

Electrical rhythmicity in the gastrointestinal tract may originate in interstitial cells of Cajal (IC). Development of IC in the small intestine is linked to signaling via the tyrosine kinase receptor, c-kit. IC express c-kit protein, and disruption of c-kit signaling causes breakdown in IC networks and loss of slow waves. We tested whether mutations in steel factor, the ligand for c-kit, affect the development of IC networks. IC were found in the region of the myenteric plexus (IC-MY) in mice with steel mutations (i.e., Sl/Sld) at 5-10 days postpartum, but these cells formed an abnormal network. IC-MY were not observed in adult Sl/Sld animals. IC in the deep muscular plexus (IC-DMP) appeared normal in Sl/Sld animals. Electrical slow waves, normally present in the small intestine, were absent in Sl/Sld animals (10-30 days postpartum). Neural inputs were intact in Sl/Sld animals. Steel factor appears important for the development of certain classes of IC, and IC-MY appear to be involved in the generation of electrical rhythmicity in the small intestine.

scholarly journals Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea

2012 ◽  
Vol 27 (3) ◽  
pp. 244-250 ◽  
Author(s):  
Sidney Pereira Pinto Lemos ◽  
José Luiz Martins ◽  
Patrícia Veruska Ribeiro Barbosa Lemos ◽  
Silvio Romero Gonçalves e Silva ◽  
Fernando Leandro dos Santos ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Myenteric Plexus ◽  
Interstitial Cells Of Cajal ◽  
Muscle Layer ◽  
Interstitial Cells ◽  
Female Rats ◽  
Nerve Plexus ◽  
Cells Of Cajal ◽  
Experimental Group

PURPOSE: The pathophysiology of abnormalities associated with myenteric plexus lesions remains imperfectly understood. Such abnormalities have been correlated with subocclusive intestinal conditions in children with Hirschsprung's disease, cases of chronic constipation and, postoperatively, in cases of anorectal anomalies. This study evaluated abnormalities of the myenteric plexus in fetus from female rats that received ethylenethiourea. METHODS: Female rats were exposed to ethylenethiourea on the 11th day of pregnancy (experimental group) or to 0.9% physiological solution (control group). Abnormalities were only found in the experimental group. The digestive tract muscle layer was analyzed morphometrically and changes to the frequencies of nerve plexus cells and interstitial cells of Cajal were evaluated, using hematoxylin-eosin, S-100 protein, neuron-specific enolase and C-Kit, respectively. RESULTS: Muscle and skeletal abnormalities were observed in 100%, anorectal anomalies in 86%, absent tail in 71%, short tail in 29%, duodenal atresia in 5%, esophageal atresia in 5% and persistent omphalomesenteric duct in 5%. Histopathological analysis showed a thinner muscle layer associated with lower frequencies of ganglion cells and interstitial cells of Cajal, in all gastrointestinal tract. CONCLUSION: Severe nerve plexus abnormalities associated with muscle layer atrophy were observed throughout the gastrointestinal tract in newborn rats exposed to ethylenethiourea.

Expression of Ca2+-activated K+channels, SK3, in the interstitial cells of Cajal in the gastrointestinal tract

2001 ◽  
Vol 281 (5) ◽  
pp. C1727-C1733 ◽  
Author(s):  
Akikazu Fujita ◽  
Tadayoshi Takeuchi ◽  
Noriko Saitoh ◽  
Jun Hanai ◽  
Fumiaki Hata
Keyword(s):  
Gastrointestinal Tract ◽  
Myenteric Plexus ◽  
Interstitial Cells Of Cajal ◽  
Circular Muscle ◽  
Microscopic Analysis ◽  
Interstitial Cells ◽  
Pcr Analysis ◽  
Sk Channels ◽  
Spontaneous Motility ◽  
Cells Of Cajal

A role for small-conductance Ca2+-activated K+ (SK) channels on spontaneous motility of the gastrointestinal tract has been suggested. Although four subtypes of SK channels were identified in mammalian tissues, the subtypes of SK channel expressed in the gastrointestinal tract are still unknown. In this study, we investigated the expression and localization of SK channels in the gastrointestinal tract. RT-PCR analysis shows expression of SK3 and SK4 mRNA, but not SK1 or SK2 mRNA, in the rat intestine. SK3 immunoreactivity was detected in the myenteric plexus and muscular layers of the stomach, ileum, and colon. SK3-immunoreactive cells were stained with antibody for c-kit, a marker for the interstitial cells of Cajal (ICC), but not with that for glial fibrillary acidic protein in the ileum and stomach. Immunoelectron microscopic analysis indicates that SK3 channels are localized on processes of ICC that are located close to the myenteric plexus between the longitudinal and circular muscle layers and within the muscular layers. Because ICC have been identified as pacemaker cells and are known to play a major role in generating the regular motility of the gastrointestinal tract, these results suggest that SK3 channels, which are expressed specifically in ICC, play an important role in generating a rhythmic pacemaker current in the gastrointestinal tract.

CD34 immunoreactivity and interstitial cells of Cajal in the human and mouse gastrointestinal tract

2000 ◽  
Vol 302 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Jean-Marie Vanderwinden ◽  
Jüri J. Rumessen ◽  
Marc-Henri De Laet ◽  
Jean-Jacques Vanderhaeghen ◽  
Serge N. Schiffmann
Keyword(s):  
Gastrointestinal Tract ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal ◽  
Human And Mouse

scholarly journals Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine

2018 ◽  
Vol 54 (1) ◽  
pp. 63
Author(s):  
Il Koo Park ◽  
Jin Ho Kim ◽  
Chan Guk Park ◽  
Man Yoo Kim ◽  
Shankar Prasad Parajuli ◽  
...  
Keyword(s):  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Pacemaker Activity ◽  
Mouse Small Intestine ◽  
Cells Of Cajal

Deficiency of interstitial cells of Cajal in the small intestine of patients with Crohn's disease

2002 ◽  
Vol 97 (1) ◽  
pp. 118-125 ◽  
Author(s):  
Christophe Porcher ◽  
Marjolaine Baldo ◽  
Monique Henry ◽  
Pierre Orsoni ◽  
Yvon Jule ◽  
...  
Keyword(s):  
Small Intestine ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal
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