scholarly journals Characteristics of the Cholecystokinin-Induced Depolarization of Pacemaking Activity in Cultured Interstitial Cells of Cajal from Murine Small Intestine

2013 ◽  
Vol 31 (4-5) ◽  
pp. 542-554 ◽  
Author(s):  
Jae Hwa Lee ◽  
Sung-Young Kim ◽  
Young Kyu Kwon ◽  
Byung Joo Kim ◽  
Insuk So
Keyword(s):  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Murine Small Intestine ◽  
Cells Of Cajal

scholarly journals Molecular markers expressed in cultured and freshly isolated interstitial cells of Cajal

2000 ◽  
Vol 279 (2) ◽  
pp. C529-C539 ◽  
Author(s):  
Anne Epperson ◽  
William J. Hatton ◽  
Brid Callaghan ◽  
Philip Doherty ◽  
Rebecca L. Walker ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Expression Profiles ◽  
Interstitial Cells ◽  
Gastric Fundus ◽  
Soluble Form ◽  
Pacemaker Activity ◽  
Murine Small Intestine ◽  
Cells Of Cajal

Located within the tunica muscularis of the gastrointestinal (GI) tract are networks of cells known as interstitial cells of Cajal (ICC). ICC are critical for important basic functions of GI motility such as generation and propagation of slow-wave pacemaker activity and reception of regulatory inputs from the enteric nervous system. We have developed a novel procedure to identify and isolate individual ICC from freshly dispersed cell preparations of the murine small intestine and gastric fundus and to determine differential transcriptional expression We have compared the expression profiles of pacemaker ICC isolated from the murine small intestine (IC-MY) and ICC involved in neurotransmission from the gastric fundus (IC-IM). We have also compared expression profiles between ICC and smooth muscle cells (SMC) and between freshly isolated ICC and cultured ICC. Cultured ICC express smooth muscle myosin, whereas freshly dispersed ICC do not. All cell types express muscarinic receptor types M2and M3, neurokinin receptors NK1and NK3, and inhibitory receptor VIP-1, whereas only cultured ICC and SMC express VIP-2. Both cultured and freshly dispersed IC-IM and IC-MY express the soluble form of stem cell factor, whereas SMC from the gastric fundus express only the membrane-bound form.

Rikkunshito Depolarizes Pacemaker Potentials of Cultured Interstitial Cells of Cajal through Ghrelin Receptors in Murine Small Intestine

Digestion ◽  
2019 ◽  
Vol 101 (3) ◽  
pp. 227-238 ◽  
Author(s):  
Jeong Nam Kim ◽  
Joo Hyun Nam ◽  
Jong Rok Lee ◽  
Sang Chan Kim ◽  
Young Kyu Kwon ◽  
...  
Keyword(s):  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Pacemaker Potentials ◽  
Ghrelin Receptors ◽  
Murine Small Intestine ◽  
Cells Of Cajal

scholarly journals Effects of gap junction inhibition on contraction waves in the murine small intestine in relation to coupled oscillator theory

2015 ◽  
Vol 308 (4) ◽  
pp. G287-G297 ◽  
Author(s):  
Sean P. Parsons ◽  
Jan D. Huizinga
Keyword(s):  
Small Intestine ◽  
Gap Junction ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Coupled Oscillator ◽  
Motor Patterns ◽  
Mapping System ◽  
Murine Small Intestine ◽  
Cells Of Cajal ◽  
Oscillator Theory

Waves of contraction in the small intestine correlate with slow waves generated by the myenteric network of interstitial cells of Cajal. Coupled oscillator theory has been used to explain steplike gradients in the frequency (frequency plateaux) of contraction waves along the length of the small intestine. Inhibition of gap junction coupling between oscillators should lead to predictable effects on these plateaux and the wave dislocation (wave drop) phenomena associated with their boundaries. It is these predictions that we wished to test. We used a novel multicamera diameter-mapping system to measure contraction along 25- to 30-cm lengths of murine small intestine. There were typically two to three plateaux per length of intestine. Dislocations could be limited to the wavefronts immediately about the terminated wave, giving the appearance of a three-pronged fork, i.e., a fork dislocation; additionally, localized decreases in velocity developed across a number of wavefronts, ending with the terminated wave, which could appear as a fork, i.e., slip dislocations. The gap junction inhibitor carbenoxolone increased the number of plateaux and dislocations and decreased contraction wave velocity. In some cases, the usual frequency gradient was reversed, with a plateau at a higher frequency than its proximal neighbor; thus fork dislocations were inverted, and the direction of propagation was reversed. Heptanol had no effect on the frequency or velocity of contractions but did reduce their amplitude. To understand intestinal motor patterns, the pacemaker network of the interstitial cells of Cajal is best evaluated as a system of coupled oscillators.

Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine

2007 ◽  
Vol 31 (3) ◽  
pp. 492-509 ◽  
Author(s):  
Hui Chen ◽  
Tamás Ördög ◽  
Junwei Chen ◽  
David L. Young ◽  
Michael R. Bardsley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Small Intestine ◽  
Motor Neurons ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cell Junction ◽  
Process Guidance ◽  
Functional Classes ◽  
Murine Small Intestine ◽  
Cells Of Cajal

Interstitial cells of Cajal (ICC) have important functions in regulation of motor activity in the gastrointestinal tract. In murine small intestine, ICC are gathered in the regions of the myenteric plexus (ICC-MY) and the deep muscular plexus (ICC-DMP). These two classes of ICC have different physiological functions. ICC-MY are pacemaker cells and generate the slow-wave electrical rhythmicity of gastrointestinal organs. ICC-DMP form synaptic connections with the varicose nerve terminals of enteric motor neurons and are involved in reception and transduction of motor neurotransmission. Gene expression underlying specific functions of ICC classes is incompletely understood. In the present study, we used recently developed highly selective techniques to isolate the two functional ICC classes from enzymatically dispersed intestinal muscles by fluorescence-activated cell sorting. The transcriptomes of ICC-MY and ICC-DMP were investigated using oligonucleotide microarray analysis. Differential expression of functional groups of genes defined by standard gene ontology terms was also studied. There were substantial numbers of genes expressed more abundantly in ICC than in the tunica muscularis, and we also detected marked phenotypic differences between ICC-MY and ICC-DMP. Notably, genes related to cell junction, process guidance, and vesicle trafficking were upregulated in ICC. Consistent with their specific functions, metabolic and Ca2+ transport genes were relatively upregulated in ICC-MY, whereas genes for signaling proteins involved in transduction of neurotransmitter functions were relatively upregulated in ICC-DMP. Our results may lead to the identification of novel biomarkers for ICC and provide directions for further studies designed to understand ICC function in health and disease.

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