scholarly journals The application of two-dimensional fluorescence correlation spectroscopy on the interaction between bovine serum albumin and prulifloxacin

2009 ◽  
Vol 23 (5-6) ◽  
pp. 257-263 ◽  
Author(s):  
Mao-Yun Xue ◽  
Ai-Ping Yang ◽  
Mei-Hua Ma ◽  
Xiao-Hua Li
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescence Spectra ◽  
Resolution Enhancement ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Fluorescence Correlation ◽  
Enthalpy Changes ◽  
2D Correlation Spectroscopy

The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.

Tracking structural transitions of bovine serum albumin in surfactant solutions by fluorescence correlation spectroscopy and fluorescence lifetime analysis

Soft Matter ◽  
2015 ◽  
Vol 11 (12) ◽  
pp. 2512-2518 ◽  
Author(s):  
Xuzhu Zhang ◽  
Andrzej Poniewierski ◽  
Sen Hou ◽  
Krzysztof Sozański ◽  
Agnieszka Wisniewska ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Bovine Serum ◽  
Structural Changes ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Surfactant Solutions ◽  
Lifetime Analysis

Sudden structural changes of BSA in surfactant solutions are observed from FCS curves.

scholarly journals Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors

The Analyst ◽  
2015 ◽  
Vol 140 (3) ◽  
pp. 765-770 ◽  
Author(s):  
Rui Lu ◽  
Wen-Wei Li ◽  
Abraham Katzir ◽  
Yosef Raichlin ◽  
Han-Qing Yu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Silver Halide ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Mid Infrared ◽  
First Time

Planar silver halide fiberoptic sensors were used for the first time for studying the mechanism of heating induced bovine serum albumin (BSA) denaturation by deconvoluted infrared attenuated total reflection (IR-ATR) spectra, and two-dimensional correlation spectroscopy (2D-CoS).

scholarly journals Investigating resonance energy transfer from protein molecules to van der Waals nanosheets

RSC Advances ◽  
2017 ◽  
Vol 7 (42) ◽  
pp. 26250-26255 ◽  
Author(s):  
Arun Singh Patel ◽  
Praveen Mishra ◽  
Pawan K. Kanaujia ◽  
Syed Shariq Husain ◽  
G. Vijaya Prakash ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Van Der Waals ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Two Dimensional ◽  
Protein Molecules ◽  
2D Nanomaterials

The resonance energy transfer (RET) from tryptophan present in bovine serum albumin (BSA) to two dimensional (2D) nanomaterials has been reported.

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