scholarly journals Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors

The Analyst ◽  
2015 ◽  
Vol 140 (3) ◽  
pp. 765-770 ◽  
Author(s):  
Rui Lu ◽  
Wen-Wei Li ◽  
Abraham Katzir ◽  
Yosef Raichlin ◽  
Han-Qing Yu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Silver Halide ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Mid Infrared ◽  
First Time

Planar silver halide fiberoptic sensors were used for the first time for studying the mechanism of heating induced bovine serum albumin (BSA) denaturation by deconvoluted infrared attenuated total reflection (IR-ATR) spectra, and two-dimensional correlation spectroscopy (2D-CoS).

Two-Dimensional Attenuated Total Reflection/Infrared Correlation Spectroscopy Studies on Concentration and Heat-Induced Structural Changes of Human Serum Albumin in Aqueous Solutions

2002 ◽  
Vol 56 (9) ◽  
pp. 1186-1193 ◽  
Author(s):  
Yuqing Wu ◽  
Koichi Murayama ◽  
Boguslawa Czarnik-Matusewicz ◽  
Yukihiro Ozaki
Keyword(s):  
Secondary Structure ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Aqueous Solutions ◽  
Ir Spectra ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Correlation Spectroscopy ◽  
Random Coil ◽  
Two Dimensional

Attenuated total reflection (ATR)/FT-IR spectra were measured for human serum albumin (HSA) in aqueous solutions (pH 6.6) with concentrations of 1.0, 2.0, 3.0, 4.0, and 5.0 wt % over a temperature range of 45–80 °C. Generalized two-dimensional (2D) correlation spectroscopy was employed to explore concentration and heat-induced structural variations of HSA in aqueous solutions. To generate 2D correlation spectra, the raw spectra were subjected to the appropriate pretreatment procedure involving ATR correction, subtraction of the spectrum of an aqueous solution, and smoothing. The synchronous and asynchronous correlation spectra were calculated for the concentration-dependent IR spectral variations in the amide I region at various temperatures. The two-dimensional ATR/IR correlation spectra greatly enhance band separation in the region and provide information about the correlation between the amide bands of HSA arising from the same and different secondary structure components. Based on the correlation investigated and previously proposed relationship between the secondary structure elements and the amide band frequencies, we have proposed the detailed assignments in the amide I region at 45 and 80 °C. The proposed assignments are compared with those based on the results of second derivative and Fourier self-deconvolution (FSD) of the ATR/IR spectra. The asynchronous spectrum generated from the concentration-dependent spectral variations at 45 °C show that side chains, the random coil, and extended chains are more sensitive than the α-helices and β-turns to the concentration change. On the other hand, the corresponding spectrum at 80 °C reveals that the conformation changes in side chains and β-turns (or β-strands) of HSA start before those in extended chain, random coil structures, and α-helices.

scholarly journals The application of two-dimensional fluorescence correlation spectroscopy on the interaction between bovine serum albumin and prulifloxacin

2009 ◽  
Vol 23 (5-6) ◽  
pp. 257-263 ◽  
Author(s):  
Mao-Yun Xue ◽  
Ai-Ping Yang ◽  
Mei-Hua Ma ◽  
Xiao-Hua Li
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescence Spectra ◽  
Resolution Enhancement ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Fluorescence Correlation ◽  
Enthalpy Changes ◽  
2D Correlation Spectroscopy

The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.

scholarly journals Investigating resonance energy transfer from protein molecules to van der Waals nanosheets

RSC Advances ◽  
2017 ◽  
Vol 7 (42) ◽  
pp. 26250-26255 ◽  
Author(s):  
Arun Singh Patel ◽  
Praveen Mishra ◽  
Pawan K. Kanaujia ◽  
Syed Shariq Husain ◽  
G. Vijaya Prakash ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Van Der Waals ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Two Dimensional ◽  
Protein Molecules ◽  
2D Nanomaterials

The resonance energy transfer (RET) from tryptophan present in bovine serum albumin (BSA) to two dimensional (2D) nanomaterials has been reported.

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