scholarly journals Correlation of Grade of Urothelial Cell Carcinomas and DNA Histogram Features Assessed by Flow Cytometry and Automated Image Cytometry

2003 ◽  
Vol 25 (3) ◽  
pp. 147-153 ◽  
Author(s):  
Marco G. W. Bol ◽  
Jan P. A. Baak ◽  
Bianca v. Diermen ◽  
E. A. M. Janssen ◽  
Susanne B. K. Buhr-Wildhagen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Multivariate Analyses ◽  
Dna Ploidy ◽  
Image Cytometry ◽  
S Phase ◽  
Urothelial Cell ◽  
Strongly Correlated ◽  
Dna Index ◽  
Fully Automatic ◽  
Dna Histogram

Objective: To analyse how DNA ploidy and S-phase fraction (SPF) by flow cytometry (FCM) and an optimised fully automatic DNA image cytometer (ICM) correlate with grade in TaT1 urothelial cell carcinomas (UC) of the urinary bladder.Materials and methods: Two-hundred-and twenty-eight consensus cases were analysed. Single cell suspensions were stained (DAPI for FCM, Feulgen for ICM). There was enough material for both FCMand ICMin 202 of these cases. FCMand optimised ICM measurements were performed on the 202 UCs. To discriminate between different grades, single- and multivariate analyses was performed on DNA histogram features obtained with the MultiCycle program (using DNA index (DI) and SPF). Results: Overall measurement time of the adapted ICM method was 10.7 minutes per case (range 5.9–29.8 min.) and required little additional interactive object rejection (average 152 objects (84–298) on 3000 objects per case measured, which took 9.9 minutes on average, range 8.3–15.5 minutes). The ICM histograms looked much “cleaner” with less noise than the FCM graphs. The coefficient of variation (CV) of the diploid peak for ICM(5.4%) was significantly lower than for FCM(5.9%) (p< 0.0001). ICM features were more strongly correlated to grade than FCMfeatures. In multivariate analysis, the best discriminating set of features was DNA ploidy and SPF (both by ICM).Conclusions: The adapted fully automated DNA ICM works very well for UCs. Low CV DNA ICM histograms are obtained in a time comparable to FCM. The DNA ICM results have stronger discriminative power than DNA FCM for grade in TaT1 UCs. Colour figures can be viewed onhttp://www.esacp.org/acp/2003/25-3/bol.htm.

scholarly journals DNA Aneuploidy by Flow Cytometry Is an Independent Prognostic Factor in Gastric Cancer

1998 ◽  
Vol 16 (4) ◽  
pp. 223-231 ◽  
Author(s):  
Mar Abad ◽  
Juana Ciudad ◽  
Manuel R. Rincon ◽  
Isabel Silva ◽  
José I. Paz-Bouza ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Prognostic Factors ◽  
Proliferative Activity ◽  
Dna Ploidy ◽  
Tumour Size ◽  
Clinical Stage ◽  
S Phase ◽  
Survival Prediction ◽  
Dna Index ◽  
Dna Aneuploidy

In the present study the prognostic value of both DNA ploidy and the proliferative activity of tomour cells were studied in a series of 76 consecutive patients suffering from gastric tumours. DNA ploidy and the proliferative index (as measured by the percentage of S-phase cells) were determined by flow cytometry using fresh tumour specimens.The presence of DNA aneuploid clones by flow cytometry was detected in 62% of the cases (mean DNA index of 1.63 ± 0.46; range 1.08–2.92), the mean proportion of S-phase cells being of 18.4 ± 11.5%. In comparison with diploid cases, aneuploid tumours showed a higher proliferative activity (cases with more than 15% S-phase cells: 18.4% versus 6.1%,p= 0.0001) as well as a higher incidence of node involvement (95% versus 68%,p= 0.001). By contrast, no significant differences were detected with respect to sex, age, histologic grade and type, clinical stage, tumour size and the incidence of extranodal involvement.Upon grouping the patients according to the proportion of S-phase cells no significant differences were observed for the clinical and biological parameters explored except for an association between a high percentage of S-phase cells and the presence of DNA aneuploidy (40% versus 96%,p= 0.0001). Regarding survival the presence of DNA aneuploidy was significantly associated with poor outcome as compared to the diploid cases (median of 15 versus 26 months,p= 0.005). By contrast, the proportion of S-phase cells did not predict patients’s outcome.Multivariate analysis of prognostic factors showed that the presence of DNA aneuploidy (p= 0.003) together with the histologic type (p= 0.03) and the existence of extranodal metastases (p= 0.05) were the best combination of prognostic factors for survival prediction.

dna flow cytometry of canine mammary tumours: the relationship of dna ploidy and S-phase fraction to clinical and histological features

1995 ◽  
Vol 58 (3) ◽  
pp. 238-243 ◽  
Author(s):  
M.D Péreze Alenza ◽  
G.R Rutteman ◽  
N.J Kuipers-Dijkshoorn ◽  
L Peña ◽  
A Montoya ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Flow Cytometry ◽  
Histological Features ◽  
Mammary Tumours ◽  
Relationship Of ◽  
The Relationship

scholarly journals Intratumoral Variations in DNA Ploidy and S-Phase Fraction in Human Breast Cancer

2001 ◽  
Vol 23 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Conny Arnerlöv ◽  
Stefan O. Emdin ◽  
Stefan Cajander ◽  
Nils‐Olof Bengtsson ◽  
Björn Tavelin ◽  
...  
Keyword(s):  
Human Breast Cancer ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Prognostic Indicators ◽  
Intratumor Heterogeneity ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Dna Index ◽  
Aneuploidy Rate

To study intratumoral DNA ploidy heterogeneity and S‐phase fraction (SPF) variability, we prospectively collected five different samples from 48 breast carcinomas and each sample was analysed separately by flow cytometry. Aneuploidy rate was 89.6% after analysis of four or five samples. DNA ploidy heterogeneity, i.e., different samples classified as either DNA euploid or DNA aneuploid in the same tumor was seen in 17%, and DNA index heterogeneity, i.e., tumor populations with different DNA indices (DIs) seen in different samples was 44%. A statistical model defining SPF heterogeneity is proposed. SPF heterogeneity as defined by us was 71%, and as expected the SPF heterogeneity rate increased significantly with increasing number of analysed samples. Four or more samples are needed to detect the most deviant (highest) SPF values. An unrecognized intratumor heterogeneity of DNA ploidy and SPF may partly explain the conflicting results reported in the literature on the above prognostic indicators.

Do Ki67, S-phase, S + G2M and DNA ploidy, evaluated by flow cytometry, reveal locoregional metastasis in oral cavity and oropharynx carcinomas?

2002 ◽  
Author(s):  
Giancarlo Tirelli ◽  
Laura Sidari ◽  
Vittorio Giacomarra ◽  
Ledia Papanikolla ◽  
Franco Sasso ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Oral Cavity ◽  
Dna Ploidy ◽  
S Phase

DNA ploidy and S-phase in recurrent astrocytomas: a retrospective study by flow cytometry of deparaffinized specimens

1996 ◽  
Vol 30 (1) ◽  
Author(s):  
Ludek Vavruch ◽  
Bo Nordenskj�ld ◽  
John Carstensen ◽  
Sverker Enestr�m
Keyword(s):  
Flow Cytometry ◽  
Retrospective Study ◽  
Dna Ploidy ◽  
S Phase

Silver-Stained Nucleolar Organizer Regions in Normal and Dysplastic Cervical Lesions: Correlation with DNA Ploidy and S-Phase Fraction by Flow Cytometry

Oncology ◽  
2006 ◽  
Vol 71 (5-6) ◽  
pp. 411-416 ◽  
Author(s):  
Madhulika Singh ◽  
Sahdeo Prasad ◽  
Neetu Kalra ◽  
Uma Singh ◽  
Yogeshwer Shukla
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
Nucleolar Organizer ◽  
S Phase ◽  
Phase Fraction ◽  
Nucleolar Organizer Regions ◽  
Cervical Lesions

T1206 Comparison of Image Cytometry and Flow Cytometry for the Detection of DNA Ploidy Abnormalities in Barrett's Esophagus

2010 ◽  
Vol 138 (5) ◽  
pp. S-511
Author(s):  
Jason M. Dunn ◽  
Peter S. Rabinovitch ◽  
Dahmane Oukrif ◽  
Stephen G. Bown ◽  
Marco Novelli ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Dna Ploidy ◽  
Image Cytometry

scholarly journals DNA index and % S phase fraction in posterior uveal melanoma: A 5 year prospective study of fresh tissue using flow cytometry

Eye ◽  
1997 ◽  
Vol 11 (5) ◽  
pp. 629-634 ◽  
Author(s):  
R P S Richardson ◽  
L Lawry ◽  
R C Rees ◽  
I G Rennie
Keyword(s):  
Flow Cytometry ◽  
Prospective Study ◽  
Uveal Melanoma ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Index ◽  
Fresh Tissue
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