scholarly journals Intratumoral Variations in DNA Ploidy and S-Phase Fraction in Human Breast Cancer

2001 ◽  
Vol 23 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Conny Arnerlöv ◽  
Stefan O. Emdin ◽  
Stefan Cajander ◽  
Nils‐Olof Bengtsson ◽  
Björn Tavelin ◽  
...  
Keyword(s):  
Human Breast Cancer ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Prognostic Indicators ◽  
Intratumor Heterogeneity ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Dna Index ◽  
Aneuploidy Rate

To study intratumoral DNA ploidy heterogeneity and S‐phase fraction (SPF) variability, we prospectively collected five different samples from 48 breast carcinomas and each sample was analysed separately by flow cytometry. Aneuploidy rate was 89.6% after analysis of four or five samples. DNA ploidy heterogeneity, i.e., different samples classified as either DNA euploid or DNA aneuploid in the same tumor was seen in 17%, and DNA index heterogeneity, i.e., tumor populations with different DNA indices (DIs) seen in different samples was 44%. A statistical model defining SPF heterogeneity is proposed. SPF heterogeneity as defined by us was 71%, and as expected the SPF heterogeneity rate increased significantly with increasing number of analysed samples. Four or more samples are needed to detect the most deviant (highest) SPF values. An unrecognized intratumor heterogeneity of DNA ploidy and SPF may partly explain the conflicting results reported in the literature on the above prognostic indicators.

The Flow-Cytometric Analysis of DNA Content and S-Phase Fraction (SPF) of Human Breast Cancer

1989 ◽  
Vol 185 (5) ◽  
pp. 694-697 ◽  
Author(s):  
M. Eskelinen ◽  
S. Nordling ◽  
J. Puittinen ◽  
E. Pesonen ◽  
Y. Collan
Keyword(s):  
Breast Cancer ◽  
Dna Content ◽  
Human Breast Cancer ◽  
Flow Cytometric Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Flow Cytometric

scholarly journals TP53 mutations and S-phase fraction but not DNA-ploidy are independent prognostic indicators in laryngeal squamous cell carcinoma

2005 ◽  
Vol 206 (1) ◽  
pp. 181-188 ◽  
Author(s):  
Antonio Russo ◽  
Simona Corsale ◽  
Valentina Agnese ◽  
Marcella Macaluso ◽  
Sandra Cascio ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Prognostic Indicators ◽  
Tp53 Mutations

scholarly journals IGF-I in human breast cancer: low differentiation stage is associated with decreased IGF-I content

2002 ◽  
pp. 813-821 ◽  
Author(s):  
E Eppler ◽  
J Zapf ◽  
N Bailer ◽  
UG Falkmer ◽  
S Falkmer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epithelial Cells ◽  
Human Breast Cancer ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Morphological Criteria ◽  
Igf I ◽  
Wet Weight ◽  
Differentiation Stage

OBJECTIVE: Few investigations on the potential role of IGF-I in human breast cancer have used morphological criteria, and the data presented on the localisation of IGF-I are controversial. Moreover, little information exists on a potential correlation between local IGF-I and the grade of malignancy or prognostic factors. Therefore, we investigated the immunohistochemical localisation of IGF-I in specimens of human breast cancer tumours of the ductal type, graded as G1/G2 (well-/moderately differentiated, n=115) and G3 (poorly differentiated, n=28). METHODS: IGF-I immunoreactivity was quantified using a scaling from no (-) to numerous (+++) IGF-I-immunoreactive cells. From 29 of the G1/G2 and 17 of the G3 tumours IGF-I was also measured by RIA. Cytosolic oestrogen receptor (ER) and progesterone receptor (PR) levels, and S-phase fraction were established and related to the number of IGF-I-immunoreactive cells. RESULTS: IGF-I immunoreactivity occurred predominantly in ductal epithelial cells. Of G3 tumours, 57% exhibited IGF-I immunoreactivity as compared with 84% of G1/G2 tumours. Correspondingly, the amount of IGF-I measured by RIA was significantly lower in G3 tumours (6.9+/-0.9 ng/g wet weight) than in G1/G2 tumours (10.5+/-1.1 ng/g wet weight) (P=0.031). G1/G2 tumours exhibited a higher percentage of IGF-I-immunoreactive cells (16% -, 23% +, 41% ++, 20% +++) than G3 tumours (43% -, 37% +, 12% ++, 8% +++). When comparing the - with the +++ G1/G2 tumours, the frequency of IGF-I-immunoreactive cells was related significantly to the ER (P<0.016) and the PR (P<0.008) levels. In G1/G2 and G3 tumours, the ER and PR levels increased with the amount of IGF immunoreactivity while the S-phase fraction increased with decreasing IGF-I content. In 25% of the specimens, IGF-I immunoreactivity occurred in stromal cells, but there was no obvious difference between the different types of tumours. The survival of the G1/G2 tumour patients increased with increasing numbers of IGF-I-immunoreactive cells. CONCLUSIONS: It is concluded that IGF-I is associated with the more-differentiated type of epithelial cells and that increasing dedifferentiation goes along with decreased IGF-I content. Thus, the presence of IGF-I immunoreactivity in breast cancer epithelial cells indicates a lower degree of malignancy than the lack of IGF-I.

scholarly journals Image cytometric DNA analysis in human breast cancer analysis may add prognostic information in diploid cases with low S-Phase fraction by flow cytometry

Cytometry ◽  
1992 ◽  
Vol 13 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Bo Baldetorp ◽  
Mårten Fernö ◽  
Anders Fallenius ◽  
Ghita Fallenius-Vecchi ◽  
Ingrid Idvall ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Human Breast Cancer ◽  
Dna Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Prognostic Information

scholarly journals Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers

1998 ◽  
Vol 16 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Gabriele A. Losa ◽  
Riccardo Graber
Keyword(s):  
Cell Death ◽  
Dna Fragmentation ◽  
Apoptotic Cell ◽  
Steroid Hormone ◽  
S Phase ◽  
Phase Fraction ◽  
Breast Cancers ◽  
Proliferative Capacity ◽  
Human Breast ◽  
Dna Index

The proliferative capacity (%S‐phase fraction), DNA ploidy, apoptosis frequency (DNA fragmentation) and steroid hormone receptor status (estrogen receptor, ER; progesterone receptor, PR) of 110 samples of human breast tissues with ductal invasive carcinoma were measured using biochemical and cytofluorimetric procedures. The DNA fragmentation had a left‐skewed frequency distribution and an overall median value of 1.64%, whilst the median %S‐phase fraction was 8%. The median %DNA fragmentation and %S‐phase fraction were 1.96% and 16% in hyperdiploid tumours (n=29; DNA index >1.1) higher than in hypodiploid tumors (n=10; DNA index 0.96), 0.38% and 7.5%. DNA diploid tumours (n=71) had median %DNA fragmentation and %S‐phase values of 1.68% and 6%, consistently lower than the median values of DNA hyperdiploid tumours. The ER content of hypodiploid tumours was about one half (median: 5.9 fmol/mg) the median values in hyperdiploid (10.6 fmol/mg) and diploid tumours (14.6 fmol/mg). This may correlate with the lowest frequency of apoptosis in hypodiploid tumours, at least when measured by biochemical methods which only detect cells in the late phases of apoptosis. In contrast, the median PR was lowest in hyperdiploid tumours than in hypo and/or diploid tumours. The %S‐phase/%fragmented DNA ratio for the hypodiploid tumours was 19.7, significantly higher than the ratios for hyperdiploid (8.2) and diploid tumours (3.6). These findings indicated that there is an imbalance between proliferative capacity and cell death or growth arrest in human breast tumours. This imbalance may well be linked to a loss of steroid hormone control.

scholarly journals Comparison of MIB-1 proliferation index with S-phase fraction in human breast carcinomas

1996 ◽  
Vol 73 (5) ◽  
pp. 640-643 ◽  
Author(s):  
PA Ellis ◽  
A Makris ◽  
SA Burton ◽  
J Titley ◽  
MG Ormerod ◽  
...  
Keyword(s):  
S Phase ◽  
Phase Fraction ◽  
Proliferation Index ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Human Breast Carcinomas ◽  
Mib 1
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