DNA ploidy and S-phase in recurrent astrocytomas: a retrospective study by flow cytometry of deparaffinized specimens

1996 ◽  
Vol 30 (1) ◽  
Author(s):  
Ludek Vavruch ◽  
Bo Nordenskj�ld ◽  
John Carstensen ◽  
Sverker Enestr�m
Keyword(s):  
Flow Cytometry ◽  
Retrospective Study ◽  
Dna Ploidy ◽  
S Phase

dna flow cytometry of canine mammary tumours: the relationship of dna ploidy and S-phase fraction to clinical and histological features

1995 ◽  
Vol 58 (3) ◽  
pp. 238-243 ◽  
Author(s):  
M.D Péreze Alenza ◽  
G.R Rutteman ◽  
N.J Kuipers-Dijkshoorn ◽  
L Peña ◽  
A Montoya ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Flow Cytometry ◽  
Histological Features ◽  
Mammary Tumours ◽  
Relationship Of ◽  
The Relationship

scholarly journals DNA Aneuploidy by Flow Cytometry Is an Independent Prognostic Factor in Gastric Cancer

1998 ◽  
Vol 16 (4) ◽  
pp. 223-231 ◽  
Author(s):  
Mar Abad ◽  
Juana Ciudad ◽  
Manuel R. Rincon ◽  
Isabel Silva ◽  
José I. Paz-Bouza ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Prognostic Factors ◽  
Proliferative Activity ◽  
Dna Ploidy ◽  
Tumour Size ◽  
Clinical Stage ◽  
S Phase ◽  
Survival Prediction ◽  
Dna Index ◽  
Dna Aneuploidy

In the present study the prognostic value of both DNA ploidy and the proliferative activity of tomour cells were studied in a series of 76 consecutive patients suffering from gastric tumours. DNA ploidy and the proliferative index (as measured by the percentage of S-phase cells) were determined by flow cytometry using fresh tumour specimens.The presence of DNA aneuploid clones by flow cytometry was detected in 62% of the cases (mean DNA index of 1.63 ± 0.46; range 1.08–2.92), the mean proportion of S-phase cells being of 18.4 ± 11.5%. In comparison with diploid cases, aneuploid tumours showed a higher proliferative activity (cases with more than 15% S-phase cells: 18.4% versus 6.1%,p= 0.0001) as well as a higher incidence of node involvement (95% versus 68%,p= 0.001). By contrast, no significant differences were detected with respect to sex, age, histologic grade and type, clinical stage, tumour size and the incidence of extranodal involvement.Upon grouping the patients according to the proportion of S-phase cells no significant differences were observed for the clinical and biological parameters explored except for an association between a high percentage of S-phase cells and the presence of DNA aneuploidy (40% versus 96%,p= 0.0001). Regarding survival the presence of DNA aneuploidy was significantly associated with poor outcome as compared to the diploid cases (median of 15 versus 26 months,p= 0.005). By contrast, the proportion of S-phase cells did not predict patients’s outcome.Multivariate analysis of prognostic factors showed that the presence of DNA aneuploidy (p= 0.003) together with the histologic type (p= 0.03) and the existence of extranodal metastases (p= 0.05) were the best combination of prognostic factors for survival prediction.

Do Ki67, S-phase, S + G2M and DNA ploidy, evaluated by flow cytometry, reveal locoregional metastasis in oral cavity and oropharynx carcinomas?

2002 ◽  
Author(s):  
Giancarlo Tirelli ◽  
Laura Sidari ◽  
Vittorio Giacomarra ◽  
Ledia Papanikolla ◽  
Franco Sasso ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Oral Cavity ◽  
Dna Ploidy ◽  
S Phase

Silver-Stained Nucleolar Organizer Regions in Normal and Dysplastic Cervical Lesions: Correlation with DNA Ploidy and S-Phase Fraction by Flow Cytometry

Oncology ◽  
2006 ◽  
Vol 71 (5-6) ◽  
pp. 411-416 ◽  
Author(s):  
Madhulika Singh ◽  
Sahdeo Prasad ◽  
Neetu Kalra ◽  
Uma Singh ◽  
Yogeshwer Shukla
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
Nucleolar Organizer ◽  
S Phase ◽  
Phase Fraction ◽  
Nucleolar Organizer Regions ◽  
Cervical Lesions

scholarly journals Correlation of Grade of Urothelial Cell Carcinomas and DNA Histogram Features Assessed by Flow Cytometry and Automated Image Cytometry

2003 ◽  
Vol 25 (3) ◽  
pp. 147-153 ◽  
Author(s):  
Marco G. W. Bol ◽  
Jan P. A. Baak ◽  
Bianca v. Diermen ◽  
E. A. M. Janssen ◽  
Susanne B. K. Buhr-Wildhagen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Multivariate Analyses ◽  
Dna Ploidy ◽  
Image Cytometry ◽  
S Phase ◽  
Urothelial Cell ◽  
Strongly Correlated ◽  
Dna Index ◽  
Fully Automatic ◽  
Dna Histogram

Objective: To analyse how DNA ploidy and S-phase fraction (SPF) by flow cytometry (FCM) and an optimised fully automatic DNA image cytometer (ICM) correlate with grade in TaT1 urothelial cell carcinomas (UC) of the urinary bladder.Materials and methods: Two-hundred-and twenty-eight consensus cases were analysed. Single cell suspensions were stained (DAPI for FCM, Feulgen for ICM). There was enough material for both FCMand ICMin 202 of these cases. FCMand optimised ICM measurements were performed on the 202 UCs. To discriminate between different grades, single- and multivariate analyses was performed on DNA histogram features obtained with the MultiCycle program (using DNA index (DI) and SPF). Results: Overall measurement time of the adapted ICM method was 10.7 minutes per case (range 5.9–29.8 min.) and required little additional interactive object rejection (average 152 objects (84–298) on 3000 objects per case measured, which took 9.9 minutes on average, range 8.3–15.5 minutes). The ICM histograms looked much “cleaner” with less noise than the FCM graphs. The coefficient of variation (CV) of the diploid peak for ICM(5.4%) was significantly lower than for FCM(5.9%) (p< 0.0001). ICM features were more strongly correlated to grade than FCMfeatures. In multivariate analysis, the best discriminating set of features was DNA ploidy and SPF (both by ICM).Conclusions: The adapted fully automated DNA ICM works very well for UCs. Low CV DNA ICM histograms are obtained in a time comparable to FCM. The DNA ICM results have stronger discriminative power than DNA FCM for grade in TaT1 UCs. Colour figures can be viewed onhttp://www.esacp.org/acp/2003/25-3/bol.htm.

DNA ploidy and percent S-phase as prognostic factors in node-positive breast cancer: results from patients enrolled in two prospective randomized trials.

1993 ◽  
Vol 11 (2) ◽  
pp. 351-359 ◽  
Author(s):  
T E Witzig ◽  
J N Ingle ◽  
D J Schaid ◽  
L E Wold ◽  
J F Barlow ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Prognostic Factors ◽  
Adjuvant Chemotherapy ◽  
Dna Ploidy ◽  
Univariate Analysis ◽  
S Phase ◽  
Node Positive ◽  
Significant Difference ◽  
Positive Breast Cancer

PURPOSE AND METHODS To help clarify the clinical utility of flow-cytometric parameters, we performed flow cytometry on archival paraffin-embedded primary breast cancers from 502 patients treated on two adjuvant chemotherapy protocols performed by the North Central Cancer Treatment Group (NCCTG) and Mayo Clinic. DNA ploidy and percent S-phase (%S) were examined in univariate and Cox model multivariate analyses along with tumor size, menopausal and estrogen receptor status, Quetelet's index (QI), number of positive nodes and nodes examined, and Fisher and nuclear grades. RESULTS Ploidy analysis showed that 40% of tumors were DNA diploid and 60% were DNA nondiploid (12% tetraploid and 48% aneuploid). There was no difference in relapse-free survival (RFS) (P = .82) or overall survival (OS) (P = .78) between the ploidy groups. Tetraploid patients had the longest RFS and OS of any group, but this did not achieve statistical significance. The %S was computed in 98% of cases and the medians were 9.0% for all patients, 6.4% for diploid patients, and 11.7% for nondiploid patients (P < .0001). By use of a %S greater than 12.3 as a prognostic variable in a univariate analysis, there was a significant difference in the RFS (P = .02) and OS (P = .007) of patients with low- versus high-proliferative tumors. However, when the %S was adjusted for clinical characteristics in the multivariate analysis, it was not a significant factor for RFS (P = .23) or OS (P = .36). CONCLUSION These results indicate that DNA content and %S measurements by flow cytometry are not clinically useful independent prognostic factors in women with resected node-positive breast cancer administered adjuvant chemotherapy.

Prognostic value of flow cytometry and correlation to some conventional prognostic factors: a retrospective study of archival specimens of 134 astrocytomas

1996 ◽  
Vol 85 (1) ◽  
pp. 146-151 ◽  
Author(s):  
Ludek Vavruch ◽  
Bo Nordenskjöld ◽  
John Carstensen ◽  
Sverker Eneström
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Who Grade ◽  
Content Type ◽  
Grade Ii ◽  
Grade Of Malignancy ◽  
Dna Pattern ◽  
Fine Print

✓ The S-phase fraction and the DNA ploidy type in 134 astrocytomas (18 Grade II, 46 Grade III, and 70 Grade IV astrocytomas) were studied using flow cytometry in a retrospective study of archival tumor specimens. A high grade of malignancy was associated with both a high S-phase fraction (p < 0.0001) and an aneuploid DNA pattern (p < 0.0001). There was no aneuploid DNA pattern found in the fibrillary astrocytomas (World Health Organization (WHO) Grade II); whereas the aneuploid pattern was observed in 80% of all the glioblastomas multiforme (WHO Grade IV). The age and gender of the patients were not significantly related to the flow cytometry parameters. The survival of patients with Grade II or III astrocytomas was significantly longer when their tumors exhibited a tetraploid DNA pattern or had a low S-phase fraction. In patients with Grade IV tumors, there was no correlation between length of survival and either the DNA ploidy or the S-phase fraction. In a multivariate Cox regression analysis of data obtained in patients with Grade II and III astrocytomas, age, grade of malignancy, DNA ploidy, and S-phase fraction were independent prognostic factors.

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