scholarly journals Epstein-Barr Virus, Human Papillomavirus, and Flow Cytometric Cell Cycle Kinetics in Nasopharyngeal Carcinoma and Inverted Papilloma among Egyptian Patients

1998 ◽  
Vol 14 (2) ◽  
pp. 113-120 ◽  
Author(s):  
S. K. Kassim ◽  
S. A. Ibrahim ◽  
S. Eissa ◽  
S. S. A. Zaki ◽  
M. A. El-Begermy ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Human Papillomavirus ◽  
Nasopharyngeal Carcinoma ◽  
Tumor Recurrence ◽  
Epstein Barr Virus ◽  
Inverted Papilloma ◽  
Hpv 16 ◽  
Primary Tumors ◽  
Barr Virus ◽  
Epstein Barr

It is widely accepted that the Epstein-Barr virus is etiologically associated with the development of nasopharyngeal carcinoma. The human papillomavirus is also associated with inverted papilloma. We used the polymerase chain reaction technique to detect both viruses in both types of tumors. Flow cytometry was also used to study the DNA pattern and proliferative behavior of the tumors in relation to the viruses. EBV was detected in 13/20 (65%) of NPC specimens, and in none of IP (n= 10) or control specimens (n= 10). This indicates the contribution of EBV as an etiologic factor in NPC. Five cases of NPC (25%) were positive for HPV 16, two of them were EBV positive. Four HPV 16 positive cases were found among cases with inverted papilloma, but none among the control cases. Flow cytometry revealed that all NPC, IP, and control samples were diploid except one aneuploid NPC sample. Proliferative capacity (PC) of primary tumors was predictive of tumor recurrence in NPC. Using 13.6% as a cut-off point for PC, we were able to discriminate between high risk and low risk groups with 100% sensitivity and 86% specificity. PC can be used as a baseline prognostic parameter in NPC, making it possible to modify courses of treatment in an attempt to inhibit tumor recurrence.

scholarly journals Epstein‐Barr virus and human papillomavirus serum antibodies define the viral status of nasopharyngeal carcinoma in a low endemic country

2020 ◽  
Vol 147 (2) ◽  
pp. 461-471 ◽  
Author(s):  
Julia Simon ◽  
Lea Schroeder ◽  
Kate Ingarfield ◽  
Stefan Diehl ◽  
Jill Werner ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Endemic Country ◽  
Serum Antibodies ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals 18F-FDG PET/CTvs. human papillomavirus, p16 and Epstein-Barr virus detection in cervical metastatic lymph nodes for identifying primary tumors

2017 ◽  
Vol 140 (6) ◽  
pp. 1405-1412 ◽  
Author(s):  
Gi Cheol Park ◽  
Jong-Lyel Roh ◽  
Kyung-Ja Cho ◽  
Jae Seung Kim ◽  
Mi Hyeon Jin ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Lymph Nodes ◽  
Fdg Pet ◽  
Epstein Barr Virus ◽  
Virus Detection ◽  
Primary Tumors ◽  
Metastatic Lymph Nodes ◽  
Barr Virus ◽  
18F Fdg ◽  
Epstein Barr

scholarly journals Human papillomavirus and Epstein-Barr virus in nasopharyngeal carcinoma in a non-endemic eastern european population

Neoplasma ◽  
2016 ◽  
Vol 63 (01) ◽  
pp. 107-114 ◽  
Author(s):  
M. SVAJDLER ◽  
J. KASPIRKOVA ◽  
R. MEZENCEV ◽  
J. LACO ◽  
T. TORDAY ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
European Population ◽  
Eastern European ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Comprehensive Profiling of Epstein-Barr Virus MicroRNAs in Nasopharyngeal Carcinoma

2008 ◽  
Vol 83 (5) ◽  
pp. 2357-2367 ◽  
Author(s):  
Katherine Cosmopoulos ◽  
Michiel Pegtel ◽  
Jared Hawkins ◽  
Howell Moffett ◽  
Carl Novina ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Pcr Assay ◽  
Primary Tumors ◽  
Barr Virus ◽  
Reverse Transcription Pcr ◽  
Copy Numbers ◽  
Wide Range ◽  
Epstein Barr

ABSTRACT Epstein-Barr Virus (EBV) establishes a long-term latent infection and is associated with a number of human malignancies that are thought to arise from deregulation of different stages of the viral life cycle. Recently, a large number of microRNAs (miRNAs) have been described for EBV, and it has been suggested that their expression may vary between the different latency states found in normal and malignant tissue. To date, however, no technique has been utilized to comprehensively and quantitatively test this idea by profiling expression of the EBV miRNAs in primary infected tissues. We describe here a multiplex reverse transcription-PCR assay that allows the profiling of 39 of the 40 known mature EBV miRNAs from as little as 250 ng of RNA. With this approach, we present a comprehensive profile of EBV miRNAs in primary nasopharyngeal carcinoma (NPC) tumors including estimates of miRNA copy number per tumor cell. This is the first comprehensive profiling of EBV miRNAs in any EBV-associated tumor. In contrast to previous suggestions, we show that the BART-derived miRNAs are present in a wide range of copy numbers from ≤103 per cell in both primary tumors and the widely used NPC-derived C666-1 cell line. However, we confirm the hypothesis that the BHRF1 miRNAs are not expressed in NPC. Lastly, we demonstrate that EBV miRNA expression in the widely used NPC line C666-1 is, with some caveats, broadly representative of primary NPC tumors.

Combined Epstein-Barr Virus and Human Papillomavirus Infection in Nasopharyngeal Carcinoma

1995 ◽  
Vol 113 (2) ◽  
pp. P76-P76
Author(s):  
Christopher H. Rassekh ◽  
Peter Rady ◽  
Stephen Tyring ◽  
Istvan Arany ◽  
Sharen Knudsen ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Human Papillomavirus Infection ◽  
Papillomavirus Infection ◽  
Epstein Barr
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