Inflammatory cells in rat skeletal muscle are elevated after electrically stimulated contractions

2003 ◽  
Vol 94 (3) ◽  
pp. 876-882 ◽  
Author(s):  
Thomas J. McLoughlin ◽  
Eleni Mylona ◽  
Troy A. Hornberger ◽  
Karyn A. Esser ◽  
Francis X. Pizza
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Sciatic Nerve ◽  
High Frequency ◽  
Tibialis Anterior ◽  
Inflammatory Cells ◽  
Muscle Contractions ◽  
Rat Skeletal Muscle ◽  
Concentric Contractions ◽  
Time Point

We determined the effect of muscle contractions resulting from high-frequency electrical stimulation (HFES) on inflammatory cells in rat tibialis anterior (TA), plantaris (Pln), and soleus (Sol) muscles at 6, 24, and 72 h post-HFES. A minimum of four and a maximum of seven rats were analyzed at each time point. HFES, applied to the sciatic nerve, caused the Sol and Pln to contract concentrically and the TA to contract eccentrically. Neutrophils were higher ( P < 0.05) at 6 and 24 h after HFES in the Sol, Pln, and TA muscles relative to control muscles. ED1+ macrophages in the Pln were elevated at 6 and 24 h after HFES and were also elevated in the Sol and TA after HFES relative to controls. ED2+ macrophages in the Sol and TA were elevated at 24 and 72 h after HFES, respectively, and were also elevated in the Pln after HFES relative to controls. In contrast to the TA muscles, the Pln and Sol muscles showed no gross histological abnormalities. Collectively, these data indicate that both eccentric and concentric contractions can increase inflammatory cells in muscle, regardless of whether overt histological signs of injury are apparent.

scholarly journals The effect of repeated bouts of electrical stimulation‐induced muscle contractions on proteolytic signaling in rat skeletal muscle

2021 ◽  
Vol 9 (9) ◽  
Author(s):  
Takaya Kotani ◽  
Junya Takegaki ◽  
Yuki Tamura ◽  
Karina Kouzaki ◽  
Koichi Nakazato ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Contractions ◽  
Rat Skeletal Muscle

Eccentric muscle contractions induce greater oxidative stress than concentric contractions in skeletal muscle

2007 ◽  
Vol 32 (2) ◽  
pp. 273-281 ◽  
Author(s):  
Michihiro Kon ◽  
Kai Tanabe ◽  
Hoseong Lee ◽  
Fuminori Kimura ◽  
Takayuki Akimoto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Tibialis Anterior ◽  
Mononuclear Cells ◽  
Tibialis Anterior Muscle ◽  
Inflammatory Cells ◽  
Muscle Contractions ◽  
Eccentric Muscle Contraction ◽  
Contraction Group

The purpose of this study was to examine oxidative stress in skeletal muscle after eccentric and concentric muscle contractions. Eight-week-old Institute of Cancer Research (ICR) mice (n = 90) were divided into 3 groups: eccentric muscle contraction group (ECC, n = 42), concentric muscle contraction group (CON, n = 42), and control group (pre, n = 6). The tibialis anterior muscle was stimulated via the peroneal nerve to contract either eccentrically or concentrically. The tibialis anterior muscle was isolated before and 0, 6, 12, 18, 24, 72, and 168 h after muscle contraction. Immediately after muscle contractions, thiobarbituric acid reactive substances (TBARS) in skeletal muscle significantly increased (p < 0.05) in both ECC and CON conditions. However, in the ECC group alone, the TBARS level peaked at 12 and 72 h after the contractions. There was greater migration of mononuclear cells in ECC than in CON muscle. In addition, there was a correlation between TBARS in skeletal muscle and migration of mononuclear cells in ECC muscle (r = 0.773, p < 0.01), but this correlation was not apparent in CON muscle (r = 0.324, p = 0.12). The increased mononuclear cells may reflect inflammatory cells. These data suggest that eccentric muscle contraction induces greater oxidative stress in skeletal muscle, which may in turn be due to enhanced generation of reactive oxygen species (ROS) by migrating inflammatory cells.

scholarly journals Eccentric contraction-induced myofiber growth in tumor-bearing mice

2016 ◽  
Vol 120 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Justin P. Hardee ◽  
Joshua E. Mangum ◽  
Song Gao ◽  
Shuichi Sato ◽  
Kimbell L. Hetzler ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Mass ◽  
High Frequency ◽  
Tibialis Anterior ◽  
Cancer Cachexia ◽  
Eccentric Contraction ◽  
Muscle Contractions ◽  
Sectional Area ◽  
Amp Kinase ◽  
Cross Sectional

Cancer cachexia is characterized by the progressive loss of skeletal muscle mass. While mouse skeletal muscle's response to an acute bout of stimulated low-frequency concentric muscle contractions is disrupted by cachexia, gaps remain in our understanding of cachexia's effects on eccentric contraction-induced muscle growth. The purpose of this study was to determine whether repeated bouts of stimulated high-frequency eccentric muscle contractions [high-frequency electrical muscle stimulation (HFES)] could stimulate myofiber growth during cancer cachexia progression, and whether this training disrupted muscle signaling associated with wasting. Male Apc Min/+ mice initiating cachexia ( N = 9) performed seven bouts of HFES-induced eccentric contractions of the left tibialis anterior muscle over 2 wk. The right tibialis anterior served as the control, and mice were killed 48 h after the last stimulation. Age-matched C57BL/6 mice ( N = 9) served as wild-type controls. Apc Min/+ mice lost body weight, muscle mass, and type IIA, IIX, and IIB myofiber cross-sectional area. HFES increased myofiber cross-sectional area of all fiber types, regardless of cachexia. Cachexia increased muscle noncontractile tissue, which was attenuated by HFES. Cachexia decreased the percentage of high succinate dehydrogenase activity myofibers, which was increased by HFES, regardless of cachexia. While cachexia activated AMP kinase, STAT3, and ERK1/2 signaling, HFES decreased AMP kinase phosphorylation, independent of the suppression of STAT3. These results demonstrate that cachectic skeletal muscle can initiate a growth response to repeated eccentric muscle contractions, despite the presence of a systemic cachectic environment.

Effect of reinnervation on collagen synthesis in rat skeletal muscle

1992 ◽  
Vol 72 (6) ◽  
pp. 2069-2074 ◽  
Author(s):  
P. Virtanen ◽  
U. Tolonen ◽  
J. Savolainen ◽  
T. E. Takala
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Tibialis Anterior ◽  
Collagen Synthesis ◽  
Tibialis Anterior Muscle ◽  
Control Level ◽  
Synthesis Rate ◽  
Collagen Biosynthesis ◽  
Denervation Atrophy ◽  
Rat Skeletal Muscle

The effect of reinnervation on the activities of prolyl 4-hydroxylase (PH) and galactosylhydroxylysyl glucosyltransferase (GGT), both enzymes of collagen biosynthesis, and on the concentration of hydroxyproline (Hyp) was studied in gastrocnemius, soleus, and tibialis anterior muscles of rat 19, 26, 40, and 61 days after crush denervation of the sciatic nerve. The GGT activity was elevated in denervated gastrocnemius and soleus muscles and the PH activity in gastrocnemius. Muscular Hyp concentration was increased in denervated tibialis anterior muscle. Both the PH and GGT activities and the Hyp concentration returned to the control level during the reinnervation period (19–61 days from the start of denervation). It seems that denervation atrophy of skeletal muscle is associated with an increased rate of muscular collagen biosynthesis and that during reinnervation collagen synthesis rate decreases despite accelerated muscular growth. The results thus suggest that innervation is a powerful suppressive regulator of muscular collagen biosynthesis.

mRNA levels for α-subunit of prolyl 4-hydroxylase and fibrillar collagens in immobilized rat skeletal muscle

1999 ◽  
Vol 87 (1) ◽  
pp. 90-96 ◽  
Author(s):  
Xiao-Yan Han ◽  
Wei Wang ◽  
Raili Myllylä ◽  
Paula Virtanen ◽  
Jarmo Karpakka ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tibialis Anterior ◽  
Collagen Synthesis ◽  
Plantar Flexion ◽  
Mrna Level ◽  
Type I ◽  
Mrna Levels ◽  
Rat Skeletal Muscle ◽  
Α Subunit ◽  
Fibrillar Collagens

There is evidence that immobilization causes a decrease in total collagen synthesis in skeletal muscle within a few days. In this study, early immobilization effects on the expression of prolyl 4-hydroxylase (PH) and the main fibrillar collagens at mRNA and protein levels were investigated in rat skeletal muscle. The right hindlimb was immobilized in full plantar flexion for 1, 3, and 7 days. Steady-state mRNAs for α- and β-subunits of PH and type I and III procollagen, PH activity, and collagen content were measured in gastrocnemius and plantaris muscles. Type I and III procollagen mRNAs were also measured in soleus and tibialis anterior muscles. The mRNA level for the PH α-subunit decreased by 49 and 55% ( P < 0.01) in gastrocnemius muscle and by 41 and 39% ( P < 0.05) in plantaris muscle after immobilization for 1 and 3 days, respectively. PH activity was decreased ( P < 0.05–0.01) in both muscles at days 3 and 7. The mRNA levels for type I and III procollagen were decreased by 26–56% ( P < 0.05–0.001) in soleus, tibialis anterior, and plantaris muscles at day 3. The present results thus suggest that pretranslational downregulation plays a key role in fibrillar collagen synthesis in the early phase of immobilization-induced muscle atrophy.

AMP-activated protein kinase activity and glucose uptake in rat skeletal muscle

2001 ◽  
Vol 280 (5) ◽  
pp. E677-E684 ◽  
Author(s):  
Nicolas Musi ◽  
Tatsuya Hayashi ◽  
Nobuharu Fujii ◽  
Michael F. Hirshman ◽  
Lee A. Witters ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Kinase ◽  
Glucose Uptake ◽  
Muscle Contractions ◽  
Treadmill Running ◽  
Dependent Manner ◽  
Rat Skeletal Muscle ◽  
Amp Activated Protein Kinase ◽  
Dose Dependent

The AMP-activated protein kinase (AMPK) has been hypothesized to mediate contraction and 5-aminoimidazole-4-carboxamide 1-β-d-ribonucleoside (AICAR)-induced increases in glucose uptake in skeletal muscle. The purpose of the current study was to determine whether treadmill exercise and isolated muscle contractions in rat skeletal muscle increase the activity of the AMPKα1 and AMPKα2 catalytic subunits in a dose-dependent manner and to evaluate the effects of the putative AMPK inhibitors adenine 9-β-d-arabinofuranoside (ara-A), 8-bromo-AMP, and iodotubercidin on AMPK activity and 3- O-methyl-d-glucose (3-MG) uptake. There were dose-dependent increases in AMPKα2 activity and 3-MG uptake in rat epitrochlearis muscles with treadmill running exercise but no effect of exercise on AMPKα1 activity. Tetanic contractions of isolated epitrochlearis muscles in vitro significantly increased the activity of both AMPK isoforms in a dose-dependent manner and at a similar rate compared with increases in 3-MG uptake. In isolated muscles, the putative AMPK inhibitors ara-A, 8-bromo-AMP, and iodotubercidin fully inhibited AICAR-stimulated AMPKα2 activity and 3-MG uptake but had little effect on AMPKα1 activity. In contrast, these compounds had absent or minimal effects on contraction-stimulated AMPKα1 and -α2 activity and 3-MG uptake. Although the AMPKα1 and -α2 isoforms are activated during tetanic muscle contractions in vitro, in fast-glycolytic fibers, the activation of AMPKα2-containing complexes may be more important in regulating exercise-mediated skeletal muscle metabolism in vivo. Development of new compounds will be required to study contraction regulation of AMPK by pharmacological inhibition.

scholarly journals Classical and adaptive control of ex vivo skeletal muscle contractions using Functional Electrical Stimulation (FES)

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0172761
Author(s):  
Paola Jaramillo Cienfuegos ◽  
Adam Shoemaker ◽  
Robert W. Grange ◽  
Nicole Abaid ◽  
Alexander Leonessa
Keyword(s):  
Skeletal Muscle ◽  
Adaptive Control ◽  
Electrical Stimulation ◽  
Functional Electrical Stimulation ◽  
Ex Vivo ◽  
Muscle Contractions

scholarly journals Diabetic Zucker rat Tibialis anterior muscle high-frequency electrical stimulation (HFES) data: Regulation of MAPKs associated proteins

Data in Brief ◽  
2018 ◽  
Vol 16 ◽  
pp. 346-353 ◽  
Author(s):  
Gautam K. Ginjupalli ◽  
Kevin M. Rice ◽  
Anjaiah Katta ◽  
Nandini D.P.K. Manne ◽  
Ravikumar Arvapalli ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
High Frequency ◽  
Tibialis Anterior ◽  
Tibialis Anterior Muscle ◽  
Zucker Rat ◽  
Associated Proteins
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