scholarly journals Low-Cost Microfluidic Arrays for Protein-Based Cancer Diagnostics Using ECL Detection

2016 ◽  
Vol 25 (2) ◽  
pp. 47-51 ◽  
Author(s):  
J. F. Rusling
Keyword(s):  
Low Cost ◽  
Cancer Diagnostics ◽  
Microfluidic Arrays

scholarly journals Molecularly imprinted polymers in biological applications

BioTechniques ◽  
2020 ◽  
Vol 69 (6) ◽  
pp. 406-419
Author(s):  
Zahra El-Schich ◽  
Yuecheng Zhang ◽  
Marek Feith ◽  
Sarah Beyer ◽  
Louise Sternbæk ◽  
...  
Keyword(s):  
Amino Acids ◽  
Molecularly Imprinted Polymers ◽  
Low Cost ◽  
Cancer Diagnostics ◽  
Biological Applications ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Recognition Elements ◽  
Synthesis Procedure

Molecularly imprinted polymers (MIPs) are currently widely used and further developed for biological applications. The MIP synthesis procedure is a key process, and a wide variety of protocols exist. The templates that are used for imprinting vary from the smallest glycosylated glycan structures or even amino acids to whole proteins or bacteria. The low cost, quick preparation, stability and reproducibility have been highlighted as advantages of MIPs. The biological applications utilizing MIPs discussed here include enzyme-linked assays, sensors, in vivo applications, drug delivery, cancer diagnostics and more. Indeed, there are numerous examples of how MIPs can be used as recognition elements similar to natural antibodies.

scholarly journals 2D Materials in Development of Electrochemical Point-of-Care Cancer Screening Devices

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 662 ◽  
Author(s):  
Mohsen Mohammadniaei ◽  
Huynh Vu Nguyen ◽  
My Van Tieu ◽  
Min-Ho Lee
Keyword(s):  
Cancer Screening ◽  
2D Materials ◽  
Point Of Care ◽  
Low Cost ◽  
Clinical Analysis ◽  
Cancer Diagnostics ◽  
Electrochemical Sensing ◽  
Screening Tools ◽  
Electrochemical Biosensors ◽  
2D Material

Effective cancer treatment requires early detection and monitoring the development progress in a simple and affordable manner. Point-of care (POC) screening can provide a portable and inexpensive tool for the end-users to conveniently operate test and screen their health conditions without the necessity of special skills. Electrochemical methods hold great potential for clinical analysis of variety of chemicals and substances as well as cancer biomarkers due to their low cost, high sensitivity, multiplex detection ability, and miniaturization aptitude. Advances in two-dimensional (2D) material-based electrochemical biosensors/sensors are accelerating the performance of conventional devices toward more practical approaches. Here, recent trends in the development of 2D material-based electrochemical biosensors/sensors, as the next generation of POC cancer screening tools, are summarized. Three cancer biomarker categories, including proteins, nucleic acids, and some small molecules, will be considered. Various 2D materials will be introduced and their biomedical applications and electrochemical properties will be given. The role of 2D materials in improving the performance of electrochemical sensing mechanisms as well as the pros and cons of current sensors as the prospective devices for POC screening will be emphasized. Finally, the future scopes of implementing 2D materials in electrochemical POC cancer diagnostics for the clinical translation will be discussed.

scholarly journals Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 536-547 ◽  
Author(s):  
Chandra K. Dixit ◽  
Karteek Kadimisetty ◽  
Brunah A. Otieno ◽  
Chi Tang ◽  
Spundana Malla ◽  
...  
Keyword(s):  
Early Detection ◽  
Low Cost ◽  
High Sensitivity ◽  
Cancer Diagnostics ◽  
Cancer Therapies

Early detection and reliable diagnostics are keys to effectively design cancer therapies with better prognoses.

Towards low-cost bioanalytical tools for sarcosine assays for cancer diagnostics

2016 ◽  
Vol 8 (40) ◽  
pp. 7312-7318 ◽  
Author(s):  
Thiago Mazzu-Nascimento ◽  
Paulo Augusto Gomes Carneiro Leão ◽  
Jonatan Ricardo Catai ◽  
Giorgio Gianini Morbioli ◽  
Emanuel Carrilho
Keyword(s):  
Prostate Cancer ◽  
Amino Acid ◽  
Low Cost ◽  
Cancer Diagnostics

Sarcosine is an amino acid that has been listed as a new indicator for prostate cancer.

scholarly journals Cell Lysis Based on an Oscillating Microbubble Array

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 288
Author(s):  
Xiufang Liu ◽  
Jinyuan Li ◽  
Liangyu Zhang ◽  
Xiaowei Huang ◽  
Umar Farooq ◽  
...  
Keyword(s):  
Cell Biology ◽  
High Speed ◽  
High Efficiency ◽  
Low Cost ◽  
Cell Lysis ◽  
Input Voltage ◽  
Disease Diagnosis ◽  
Cancer Diagnostics ◽  
Significant Difference ◽  
The Individual

Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble array that enables the realization of multiple cell lysis induced by the shear stress resulting from the individual oscillating microbubbles. The oscillating microbubbles in the channel have similar vibration amplitudes, and the intracellular substances can be released from the individual cells efficiently. Moreover, the efficiency of cell lysis increases with increments of input voltage and sonication time. By means of DNA agarose-gel electrophoresis, a sufficient extraction amount of DNA released from the lysed cells can be detected, and there is no significant difference in lysis efficiency when compared to cell lysis achieved using commercial kits. With the advantages of the simple manufacturing process, low cost, high efficiency, and high speed, this device can serve as an efficient and versatile tool for the single-cell sequencing of cell biology research, disease diagnosis, and stem cell therapy.

scholarly journals Glycoprofiling of cancer biomarkers: Label-free electrochemical lectin-based biosensors

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Dominika Pihíková ◽  
Peter Kasák ◽  
Jan Tkac
Keyword(s):  
Electrochemical Impedance ◽  
Early Stage ◽  
Point Of Care ◽  
Low Cost ◽  
Warning Signal ◽  
Cancer Biomarkers ◽  
Cancer Diagnostics ◽  
Label Free ◽  
Glycosylation Pattern ◽  
Human Proteins

AbstractGlycosylation of biomolecules is one of the most prevalent post- and co-translational modification in a human body, with more than half of all human proteins being glycosylated. Malignant transformation of cells influences glycosylation machinery resulting in subtle changes of the glycosylation pattern within the cell populations as a result of cancer. Thus, an altered terminal glycan motif on glycoproteins could provide a warning signal about disease development and progression and could be applied as a reliable biomarker in cancer diagnostics. Among all highly effective glycoprofiling tools, label-free electrochemical impedance spectroscopy (EIS)-based biosensors have emerged as especially suitable tool for point-of-care early-stage cancer detection. Herein, we highlight the current challenges in glycoprofiling of various cancer biomarkers by ultrasensitive impedimetric-based biosensors with low sample consumption, low cost fabrication and simple miniaturization. Additionally, this review provides a short introduction to the field of glycomics and lectinomics and gives a brief overview of glycan alterations in different types of cancer.

scholarly journals 3D-Printed Immunosensor Arrays for Cancer Diagnostics

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4514 ◽  
Author(s):  
Mohamed Sharafeldin ◽  
Karteek Kadimisetty ◽  
Ketki S. Bhalerao ◽  
Tianqi Chen ◽  
James F. Rusling
Keyword(s):  
3D Printing ◽  
Early Stage ◽  
Point Of Care ◽  
Low Cost ◽  
Cancer Diagnostics ◽  
Diagnostic Tools ◽  
Protein Biomarkers ◽  
Protein Biomarker ◽  
Subtractive Manufacturing ◽  
3D Printed

Detecting cancer at an early stage of disease progression promises better treatment outcomes and longer lifespans for cancer survivors. Research has been directed towards the development of accessible and highly sensitive cancer diagnostic tools, many of which rely on protein biomarkers and biomarker panels which are overexpressed in body fluids and associated with different types of cancer. Protein biomarker detection for point-of-care (POC) use requires the development of sensitive, noninvasive liquid biopsy cancer diagnostics that overcome the limitations and low sensitivities associated with current dependence upon imaging and invasive biopsies. Among many endeavors to produce user-friendly, semi-automated, and sensitive protein biomarker sensors, 3D printing is rapidly becoming an important contemporary tool for achieving these goals. Supported by the widely available selection of affordable desktop 3D printers and diverse printing options, 3D printing is becoming a standard tool for developing low-cost immunosensors that can also be used to make final commercial products. In the last few years, 3D printing platforms have been used to produce complex sensor devices with high resolution, tailored towards researchers’ and clinicians’ needs and limited only by their imagination. Unlike traditional subtractive manufacturing, 3D printing, also known as additive manufacturing, has drastically reduced the time of sensor and sensor array development while offering excellent sensitivity at a fraction of the cost of conventional technologies such as photolithography. In this review, we offer a comprehensive description of 3D printing techniques commonly used to develop immunosensors, arrays, and microfluidic arrays. In addition, recent applications utilizing 3D printing in immunosensors integrated with different signal transduction strategies are described. These applications include electrochemical, chemiluminescent (CL), and electrochemiluminescent (ECL) 3D-printed immunosensors. Finally, we discuss current challenges and limitations associated with available 3D printing technology and future directions of this field.

scholarly journals Recent Research Trends and Future Prospects in Nanozymes

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Ho Yun Shin ◽  
Tae Jung Park ◽  
Moon Il Kim
Keyword(s):  
Small Molecules ◽  
Low Cost ◽  
Scale Up ◽  
Cancer Diagnostics ◽  
Research Trends ◽  
Environmental Engineering ◽  
Future Prospects ◽  
Treatment Methods ◽  
Natural Enzyme ◽  
Enzyme Mimetics

Recently, nanomaterial-based enzyme mimetics (nanozymes) have attracted enormous interest. They exhibit unique advantages such as excellent robustness, stability, and low-cost production with easy scale-up, which are critically needed as an alternative to natural enzymes. These nanozymes exhibit natural enzyme-like activity and have been applied to various kinds of detection and treatment methods for biomolecules such as DNA, proteins, cells, and small molecules including glucose. To highlight progress in the field of nanozymes, this review discusses recent nanozyme-based research results and their applications for the development of novel biosensor, immunoassay, cancer diagnostics, therapeutics, and environmental engineering technologies. Current challenges and future prospects of nanozymes for widespread use in biotechnology are also discussed.

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