A Nitrocellulose-Based Microfluidic Device for Generation of Concentration Gradients and Study of Bacterial Chemotaxis

2013 ◽  
Vol 161 (2) ◽  
pp. B3064-B3070 ◽  
Author(s):  
Alireza Mahdavifar ◽  
Jie Xu ◽  
Mona Hovaizi ◽  
Peter Hesketh ◽  
Wayne Daley ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Bacterial Chemotaxis ◽  
Concentration Gradients

Microfluidic device for generating regionalized concentration gradients under a stable and uniform fluid microenvironment

2018 ◽  
Vol 29 (1) ◽  
pp. 015008 ◽  
Author(s):  
Juan Wei ◽  
Chong Liu ◽  
Yang Jiang ◽  
Chunzheng Duan ◽  
Li Chen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Concentration Gradients ◽  
Uniform Fluid

A microfluidic device mimicking acinar concentration gradients across the liver acinus

2013 ◽  
Vol 15 (5) ◽  
pp. 767-780 ◽  
Author(s):  
Ming-Cheng Shih ◽  
Shih-Heng Tseng ◽  
Yu-Shih Weng ◽  
I-Ming Chu ◽  
Cheng-Hsien Liu
Keyword(s):  
Microfluidic Device ◽  
Concentration Gradients ◽  
Liver Acinus

Quantifying orientational regeneration of injured neurons by natural product concentration gradients in a 3D microfluidic device

Lab on a Chip ◽  
2018 ◽  
Vol 18 (6) ◽  
pp. 971-978 ◽  
Author(s):  
Yun Tang ◽  
Quan-Fa Qiu ◽  
Fu-Li Zhang ◽  
Min Xie ◽  
Wei-Hua Huang
Keyword(s):  
Central Nervous System ◽  
Extracellular Matrix ◽  
Nervous System ◽  
Small Molecules ◽  
Natural Product ◽  
Microfluidic Device ◽  
Concentration Gradients ◽  
Product Concentration

We developed a microfluidic device which can provide multiple adjustable gradients in a 3D extracellular matrix to investigate regeneration of injured central nervous system neurons in response to natural small molecules.

1P1-L09 Microfluidic Device to Observe Behavior of Cancer Cells under Concentration Gradients of Glucose and Oxygen

2015 ◽  
Vol 2015 (0) ◽  
pp. _1P1-L09_1-_1P1-L09_3
Author(s):  
Tadashi ISHIDA ◽  
Nobuya OZAKI ◽  
Takahiro KUCHIMARU ◽  
Shinae KONDOH ◽  
Toru OMATA
Keyword(s):  
Cancer Cells ◽  
Microfluidic Device ◽  
Concentration Gradients

scholarly journals A Microfluidic Device to Establish Concentration Gradients Using Reagent Density Differences

2010 ◽  
Vol 132 (12) ◽  
Author(s):  
Qingjun Kong ◽  
Richard A. Able ◽  
Veronica Dudu ◽  
Maribel Vazquez
Keyword(s):  
Numerical Simulations ◽  
Transport Properties ◽  
Microfluidic Device ◽  
Life Science ◽  
Chemical Concentration ◽  
Spatial Concentration ◽  
Concentration Gradients ◽  
Transport Models ◽  
Microfluidic Flow ◽  
Over Time

Microfabrication has become widely utilized to generate controlled microenvironments that establish chemical concentration gradients for a variety of engineering and life science applications. To establish microfluidic flow, the majority of existing devices rely upon additional facilities, equipment, and excessive reagent supplies, which together limit device portability as well as constrain device usage to individuals trained in technological disciplines. The current work presents our laboratory-developed bridged μLane system, which is a stand-alone device that runs via conventional pipette loading and can operate for several days without need of external machinery or additional reagent volumes. The bridged μLane is a two-layer polydimethylsiloxane microfluidic device that is able to establish controlled chemical concentration gradients over time by relying solely upon differences in reagent densities. Fluorescently labeled Dextran was used to validate the design and operation of the bridged μLane by evaluating experimentally measured transport properties within the microsystem in conjunction with numerical simulations and established mathematical transport models. Results demonstrate how the bridged μLane system was used to generate spatial concentration gradients that resulted in an experimentally measured Dextran diffusivity of (0.82±0.01)×10−6 cm2/s.

scholarly journals Flow-Based Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable, Competing Gradients

2009 ◽  
Vol 75 (13) ◽  
pp. 4557-4564 ◽  
Author(s):  
Derek L. Englert ◽  
Michael D. Manson ◽  
Arul Jayaraman
Keyword(s):  
Microfluidic Device ◽  
Fluorescent Protein ◽  
Bacterial Colonization ◽  
Bacterial Chemotaxis ◽  
E Coli ◽  
Autoinducer 2 ◽  
Phase Signal ◽  
Green Fluorescent ◽  
Diffusive Mixing ◽  
Migration Of Cells

ABSTRACT Chemotaxis is the migration of cells in gradients of chemoeffector molecules. Although multiple, competing gradients must often coexist in nature, conventional approaches for investigating bacterial chemotaxis are suboptimal for quantifying migration in response to gradients of multiple signals. In this work, we developed a microfluidic device for generating precise and stable gradients of signaling molecules. We used the device to investigate the effects of individual and combined chemoeffector gradients on Escherichia coli chemotaxis. Laminar flow-based diffusive mixing was used to generate gradients, and the chemotactic responses of cells expressing green fluorescent protein were determined using fluorescence microscopy. Quantification of the migration profiles indicated that E. coli was attracted to the quorum-sensing molecule autoinducer-2 (AI-2) but was repelled from the stationary-phase signal indole. Cells also migrated toward higher concentrations of isatin (indole-2,3-dione), an oxidized derivative of indole. Attraction to AI-2 overcame repulsion by indole in equal, competing gradients. Our data suggest that concentration-dependent interactions between attractant and repellent signals may be important determinants of bacterial colonization of the gut.

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