Construction of oxygen and chemical concentration gradients in a single microfluidic device for studying tumor cell–drug interactions in a dynamic hypoxia microenvironment

Lab on a Chip ◽  
2013 ◽  
Vol 13 (4) ◽  
pp. 695-705 ◽  
Author(s):  
Lei Wang ◽  
Wenming Liu ◽  
Yaolei Wang ◽  
Jian-chun Wang ◽  
Qin Tu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Drug Interactions ◽  
Microfluidic Device ◽  
Chemical Concentration ◽  
Concentration Gradients

scholarly journals Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions

Micromachines ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 493
Author(s):  
Shaofei Shen ◽  
Fangjuan Zhang ◽  
Mengqi Gao ◽  
Yanbing Niu
Keyword(s):  
Tumor Cell ◽  
Drug Interactions ◽  
Cell Lines ◽  
Drug Concentration ◽  
Concentration Gradient ◽  
Breast Adenocarcinoma ◽  
Tumor Cell Lines ◽  
Concentration Gradients ◽  
Flow Rates ◽  
Wide Range

With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively shorten experimental time, reduce costs, improve efficiency, and achieve a large-scale, high-throughput and accurate analysis. However, most of these microfluidic technologies are established for narrow-range drug-concentration screening based on sensitive but limited flow rates. More simple, easy-to operate and wide-ranging concentration-gradient constructions for studying tumor cell–drug interactions in real-time have remained largely out of reach. Here, we proposed a simple and compact device that can quickly construct efficient and reliable drug-concentration gradients with a wide range of flow rates. The dynamic study of concentration-gradient formation based on successive spiral mixer regulations was investigated systematically and quantitatively. Accurate, stable, and controllable dual drug-concentration gradients were produced to evaluate simultaneously the efficacy of the anticancer drug against two tumor cell lines (human breast adenocarcinoma cells and human cervical carcinoma cells). Results showed that paclitaxel had dose-dependent effects on the two tumor cell lines under the same conditions, respectively. We expect this device to contribute to the development of microfluidic chips as a portable and economical product in terms of the potential of concentration gradient-related biochemical research.

scholarly journals A Microfluidic Device to Establish Concentration Gradients Using Reagent Density Differences

2010 ◽  
Vol 132 (12) ◽  
Author(s):  
Qingjun Kong ◽  
Richard A. Able ◽  
Veronica Dudu ◽  
Maribel Vazquez
Keyword(s):  
Numerical Simulations ◽  
Transport Properties ◽  
Microfluidic Device ◽  
Life Science ◽  
Chemical Concentration ◽  
Spatial Concentration ◽  
Concentration Gradients ◽  
Transport Models ◽  
Microfluidic Flow ◽  
Over Time

Microfabrication has become widely utilized to generate controlled microenvironments that establish chemical concentration gradients for a variety of engineering and life science applications. To establish microfluidic flow, the majority of existing devices rely upon additional facilities, equipment, and excessive reagent supplies, which together limit device portability as well as constrain device usage to individuals trained in technological disciplines. The current work presents our laboratory-developed bridged μLane system, which is a stand-alone device that runs via conventional pipette loading and can operate for several days without need of external machinery or additional reagent volumes. The bridged μLane is a two-layer polydimethylsiloxane microfluidic device that is able to establish controlled chemical concentration gradients over time by relying solely upon differences in reagent densities. Fluorescently labeled Dextran was used to validate the design and operation of the bridged μLane by evaluating experimentally measured transport properties within the microsystem in conjunction with numerical simulations and established mathematical transport models. Results demonstrate how the bridged μLane system was used to generate spatial concentration gradients that resulted in an experimentally measured Dextran diffusivity of (0.82±0.01)×10−6 cm2/s.

A Nitrocellulose-Based Microfluidic Device for Generation of Concentration Gradients and Study of Bacterial Chemotaxis

2013 ◽  
Vol 161 (2) ◽  
pp. B3064-B3070 ◽  
Author(s):  
Alireza Mahdavifar ◽  
Jie Xu ◽  
Mona Hovaizi ◽  
Peter Hesketh ◽  
Wayne Daley ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Bacterial Chemotaxis ◽  
Concentration Gradients

Microfluidic device for generating regionalized concentration gradients under a stable and uniform fluid microenvironment

2018 ◽  
Vol 29 (1) ◽  
pp. 015008 ◽  
Author(s):  
Juan Wei ◽  
Chong Liu ◽  
Yang Jiang ◽  
Chunzheng Duan ◽  
Li Chen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Concentration Gradients ◽  
Uniform Fluid

scholarly journals Electrolyte Metabolism in HeLa Cells

1963 ◽  
Vol 46 (6) ◽  
pp. 1303-1315 ◽  
Author(s):  
Margaret Wickson-ginzburg ◽  
A. K. Solomon
Keyword(s):  
Transport System ◽  
Hela Cells ◽  
Generation Time ◽  
Chemical Concentration ◽  
Concentration Gradients ◽  
K Uptake ◽  
Initial Value ◽  
Electrolyte Metabolism ◽  
K Cells ◽  
Low K

Methods have been developed to study cellular Na, K, and Cl concentrations in HeLa cells. Cell [Na] and [K] are functions of the age of the culture. As the culture grows [K], expressed in mmols/liter cell H2O, rises from an initial value of 121 to a peak of 206 at about 4 days, and thereafter falls until it has almost returned to the initial value by the 9th day. [Na] falls as [K] rises, but there is no fixed relationship between the cellular concentrations of the two cations. There is, however, a correlation between generation time and cellular [K]. Measurements of net K uptake and net Na extrusion were carried out during 1 hour incubation at 37°C of low K cells. Both net K uptake and net Na extrusion took place against chemical concentration gradients, so that at least one transport system must be active; if the Cl distribution is passive both net K uptake and net Na extrusion are active. Studies with inhibitors of respiration and glycolysis lead to the conclusion that respiration is not required for these net transports, which appear to derive their energy from glycolytic sources.

Three-dimensional chemical concentration maps in a microfluidic device using two-photon absorption fluorescence imaging

2007 ◽  
Vol 32 (17) ◽  
pp. 2568 ◽  
Author(s):  
Dawn Schafer ◽  
Emily A. Gibson ◽  
Wafa Amir ◽  
Rebecca Erikson ◽  
Jodi Lawrence ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Microfluidic Device ◽  
Three Dimensional ◽  
Photon Absorption ◽  
Chemical Concentration ◽  
Two Photon Absorption ◽  
Two Photon
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