Subcutaneous injection of small fish: radionuclide release from the injection site
A technique is described for the subcutaneous injection of small fish using the goldfish (Carassius auratus L.) as a model. Using radioactive tracers, (109Cd, 125I), maximum levels occur in the plasma at 4 and 16 min, with statistical plateaus evident by 2 and 4 min, respectively. The plateau level of injected material occurring in the plasma shortly after the injection exposes the tissues to a constant dose of the injected material for the duration of the plateau. In the case of 109Cd, this period is in excess of 4 h. Subcutaneous injection by this method provides low variability in delivery, rapid plasma – delivery site equilibration of the injected material, and a constant plasma level of the injected material for a significant time interval.