Subcutaneous injection of small fish: radionuclide release from the injection site

1979 ◽  
Vol 57 (3) ◽  
pp. 696-698
Author(s):  
Greg P. Busacker ◽  
Walter Chavin

A technique is described for the subcutaneous injection of small fish using the goldfish (Carassius auratus L.) as a model. Using radioactive tracers, (109Cd, 125I), maximum levels occur in the plasma at 4 and 16 min, with statistical plateaus evident by 2 and 4 min, respectively. The plateau level of injected material occurring in the plasma shortly after the injection exposes the tissues to a constant dose of the injected material for the duration of the plateau. In the case of 109Cd, this period is in excess of 4 h. Subcutaneous injection by this method provides low variability in delivery, rapid plasma – delivery site equilibration of the injected material, and a constant plasma level of the injected material for a significant time interval.

1992 ◽  
Vol 70 (8) ◽  
pp. 1585-1589 ◽  
Author(s):  
Michael J. Richardson ◽  
Frederick G. Whoriskey

Experiments were conducted to determine the effect of body size, population size and structure, and temperature upon the level of turbidity generation by goldfish foraging in laboratory pools. Water temperature correlated with turbidity generation, which peaked at 25 °C but declined at 30 °C. Goldfish exhibited diurnal activity at all temperatures except 25 °C, where nocturnal behaviour predominated. Both body length and population size positively influenced turbidity production. Groups of a few large fish produced significantly more turbidity than groups of many small fish of equal biomass. Biomass was therefore a poor predictor of the level of turbidity, making it difficult to predict the impact of goldfish upon multispecific pond communities following successful colonization.


1975 ◽  
Vol 36 (3) ◽  
pp. 979-985
Author(s):  
Allen H. Wolach ◽  
Maureen A. McHale ◽  
Clara S. Fitzpatrick ◽  
Reed Dunlap

Goldfish in Experiment 1 were placed on terminal deprivation when they reached 2, 3, 4, or 16 mo. Fish that were 4-mo.-old showed the greatest median survival time (2 mo.). Large fish lost a smaller percentage of their weight before death than small fish. Fish, unlike mammals, did not die when they lost a fixed percentage of their predeprivation weight. A second experiment was performed to assess recovery from deprivation. Feeding was reinstated for some of the fish after different numbers of days of deprivation (2, 28, 44, or 64 days). Fish that survived until feeding was reinstated recovered from deprivation.


Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.


2001 ◽  
Vol 36 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
I Paschos ◽  
L Natsis ◽  
C Nathanailides ◽  
I Kagalou ◽  
E Kolettas

2021 ◽  
Vol 66 ◽  
pp. 126758
Author(s):  
Javad Seyedi ◽  
Mohammad Reza Kalbassi ◽  
Milad Esmaeilbeigi ◽  
Mohammad Behzadi Tayemeh ◽  
Jamshid Amiri Moghadam

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