Effects of exposure to a therapeutic level of methylene blue on antioxidant capacity, haemato‐immunological responses and resistance of goldfish, Carassius auratus to Aeromonas hydrophila

2021 ◽  
Author(s):  
Siyavash Soltanian ◽  
Amin Gholamhosseini ◽  
Mahdi Banaee
Keyword(s):  
Methylene Blue ◽  
Antioxidant Capacity ◽  
Aeromonas Hydrophila ◽  
Carassius Auratus ◽  
Therapeutic Level ◽  
Immunological Responses ◽  
Goldfish Carassius Auratus

Variations of physiological and innate immunological responses in goldfish (Carassius auratus) subjected to recurrent acute stress

2014 ◽  
Vol 37 (1) ◽  
pp. 147-153 ◽  
Author(s):  
Khalil Eslamloo ◽  
Sobhan R. Akhavan ◽  
Farzin Jamalzad Fallah ◽  
Morgane A. Henry
Keyword(s):  
Carassius Auratus ◽  
Acute Stress ◽  
Immunological Responses ◽  
Goldfish Carassius Auratus

scholarly journals Isolation of Tetrahymena pyriformis From Several Infected Pet Fish Species And a Regime For Its Treatment

2005 ◽  
Vol 29 (1) ◽  
pp. 190-195
Author(s):  
Omar Bassim Ahmed Al- Tayyar
Keyword(s):  
Methylene Blue ◽  
Water Temperature ◽  
Carassius Auratus ◽  
Poecilia Reticulata ◽  
Tetrahymena Pyriformis ◽  
Infected Fish ◽  
Mucus Hypersecretion ◽  
Goldfish Carassius Auratus ◽  
Poecilia Sphenops ◽  
Pterophyllum Scalare

Tetrahymena pyriformis has been firstly isolated from brood Goldfish(Carassius auratus) Common, Fantail, Ryukin, Veiltail and Moor, Black molly(Poecilia sphenops), Gourami (Trichogaster trichopterus), red Swordtail(Xiphophorus helleri), Guppy (Poecilia reticulata), deep Angelfish(Pterophyllum scalare) and brood Algae eater (Hypostomus plecostomus) inIraq. The infected fish suffered from mucus hypersecretion large hyperemicareas on the skin, and slight shedding of the scales. The incidence percentage ofinfection was 100 %. Rising water temperature was up to 29 – 30cْ for one timeand methylene blue was at a concentration of 5 ppm for 24 hours. Sodiumchloride at a concentration of 2 % for one minute and two minutes has noobvious effect on Tetrahymena pyriformis. Treatment with NaCl at theconcentration of 1% for 20 minutes for three consecutive days achieved the bestresults.

Phytotherapy of ulcerative dermatitis induced by Aeromonas hydrophila infection in goldfish ( Carassius auratus )

2010 ◽  
Vol 58 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Ramasamy Harikrishnan ◽  
Chellam Balasundaram ◽  
Young-Gun Moon ◽  
Man-Chul Kim ◽  
Ju-Sang Kim ◽  
...  
Keyword(s):  
Body Length ◽  
Aeromonas Hydrophila ◽  
Carassius Auratus ◽  
Curcuma Longa ◽  
Treated Group ◽  
The Body ◽  
Sublethal Dose ◽  
Ocimum Sanctum ◽  
Goldfish Carassius Auratus ◽  
Successful Recovery

Goldfish ( Carassius auratus ) weighing 13 ± 2 g were administered intramuscularly a sublethal dose (1.8 × 10 3 cfu/ml) of Aeromonas hydrophila to induce ulcerative dermatitis. On day 3 and day 6 after infection the fish were dip-treated (for 5 min/day) with a tri-herbal concoction of Curcuma longa, Ocimum sanctum and Azadirachta indica (1%). The LD 50 value was recorded at a concentration of 2.3 × 10 4 between 30 and 36 days after infection. In the infected untreated group the cumulative mortality was higher, while in the early-treated group (day 3) there was no mortality. In the late-treated group (6th day) the mortality increased to 23.3% on day 36. In the infected group the size of ulcers progressively increased from 43.3% of the body length on day 18 to 86.7% on day 36. In the early-treated fish the size of ulcers was 23.3% of the body length on day 18; by day 36 after treatment the ulcer had completely healed. In contrast, there was only a moderate recovery in the late-treated group. From the results it can be concluded that early tri-herbal treatment ensures successful recovery from ulcerative dermatitis induced by A. hydrophila .

scholarly journals Aeromonas hydrophila associated with mass mortality of adult Goldfish, Carassius auratus (Linnaeus 1758) in ornamental farms in India

2018 ◽  
Vol 65 (4) ◽  
Author(s):  
Arathi Dharmaratnam ◽  
T.Raja Swaminathan ◽  
Raj Kumar ◽  
V.S. Basheer
Keyword(s):  
Cell Line ◽  
Aeromonas Hydrophila ◽  
Carassius Auratus ◽  
Tissue Homogenate ◽  
Haemolytic Activity ◽  
Fish Farms ◽  
Koi Herpesvirus ◽  
Goldfish Carassius Auratus ◽  
Rapd Profile ◽  
Acinetobacter Spp

Aeromonas hydrophila was identified the causative agent of a disease outbreak in goldfish, Carassius auratus from four ornamental fish farms in Kerala, India. Seven bacterial isolates viz., Plesiomonas shigelloides (NPPS-1), Aeromonas hydrophila (NPAH-1, 2, 3 and 4), Citrobacter freundii (NPCF-1) and Acinetobacter spp. (NPA-1) were isolated from the affected fish. Further, all four A. hydrophila (NPAH-1, 2, 3 and 4) isolates were identified by amplification of gyrB and rpoD genes. The RAPD profile using 3 primers confirmed that all four A. hydrophila were genetically similar. No cytopathic effect was observed on goldfish fin (GFF) cell line after inoculation of the tissue homogenate from the affected fish and affected tissues were found negative for koi herpesvirus (KHV), cyprinid herpesvirus-2 (CyHV-2) and spring viraemia of carp virus (SVCV). Experimental challenge resulted in mortality of fish injected with A. hydrophila only. A. hydrophila was observed to be cytotoxic on GFF cell line and exhibited haemolytic activity on 5% sheep blood agar. A. hydrophila possessed multiple virulence genes viz., enterotoxins, haemolytic toxins and outer membrane protein as determined by PCR. A. hydrophila was sensitive to Cefixime, Chloramphenicol, Nitrofurantoin, Kanamycin, Ciprofloxacin, Furazolidone and Cefixime/Clavulanic acid. After treatment with the suggested antibiotics, the fish were recovered from the disease.

Obligate Anaerobic Bacteria in the Gastrointestinal Microflora of the Grass Carp (Ctenopharyngodon idella), Goldfish (Carassius auratus), and Rainbow Trout (Salmo gairdneri)

1979 ◽  
Vol 36 (10) ◽  
pp. 1174-1179 ◽  
Author(s):  
T. J. Trust ◽  
L. M. Bull ◽  
B. R. Currie ◽  
J. T. Buckley
Keyword(s):  
Rainbow Trout ◽  
Aeromonas Hydrophila ◽  
Grass Carp ◽  
Carassius Auratus ◽  
Anaerobic Bacteria ◽  
Ctenopharyngodon Idella ◽  
Salmo Gairdneri ◽  
Goldfish Carassius Auratus ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Gastrointestinal Microflora

Grass carp (Ctenopharyngodon idella), bred in Arkansas and maintained in British Columbia under defined culture conditions on pelleted diets and on aquatic weeds, were examined to determine their commensal gastrointestinal bacterial flora. Using anaerobic incubation, the total bacterial numbers cultured ranged from 6 × 104 to 4 × 108 (average 4 × 106) per g of alimentary tract plus contents. Obligately anaerobic bacteria were isolated and identified as species of Actinomyces, Bacteroides, Eubacterium, Fusobacterium, and Peptostreptococcus. This is the first description of the presence of these strictly anaerobic organisms in the gastrointestinal tract of fish. Indeed, many of the anaerobes isolated did not conform to previously described species. Members of the Genus Clostridium were also isolated by enrichment culture and appeared to be associated with a pond weed diet. A wide variety of facultative anaerobic bacteria were also isolated, with Aeromonas hydrophila predominating. The gastrointestinal tracts of goldfish (Carassius auratus) were also found to contain sizable numbers of obligate anaerobes, but the gastrointestinal tracts of hatchery-cultured rainbow trout (Salmo gairdneri) contained insignificant numbers. Key words: grass carp, anaerobic bacteria, gastrointestinal microflora, Aeromonas hydrophila, rainbow trout, goldfish

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