CHROMOSOME ABERRATIONS IN IRRADIATED CELLS OF CHINESE HAMSTER GROWN IN TISSUE CULTURE

1960 ◽  
Vol 38 (1) ◽  
pp. 203-207 ◽  
Author(s):  
Resa Wakonig ◽  
D. K. Ford
Keyword(s):  
Tissue Culture ◽  
Chromosome Aberrations ◽  
Chromosome Complement ◽  
Chinese Hamster ◽  
Chromosome Length ◽  
Culture Technique ◽  
Tissue Cultures ◽  
Straight Line ◽  
Research Problems ◽  
Unusual Chromosome

Various types of chromosome aberrations were described and their incidence recorded from analyses of metaphases derived from irradiated tissue cultures of the Chinese hamster. The aberrations included: chromatid breaks, incomplete breaks, isolocus breaks, various types of chromatid interchanges, chromatid intrachanges, minutes, and rings. The chromosomes taking part in. various configurations could usually be identified, at least into certain groups. The aberrations encountered after irradiation were of the chromatid type. The lowest dose used was 15 rads and it caused abnormalities. The graph relating the incidence of breaks to the chromosome length was not a straight line but curved suggesting a nonproportionally large increase of breaks with the long chromosomes.The advantages of the tissue culture technique and the unusual chromosome complement of the Chinese hamster were found to be, as anticipated, of great value in this study and could be utilized in various research problems.

scholarly journals A Tissue Culture Study of Morphine Dependence on the Mammalian CNS

1969 ◽  
Vol 14 (6) ◽  
pp. 607-615 ◽  
Author(s):  
A. Ghadirian
Keyword(s):  
Tissue Culture ◽  
Nervous Tissue ◽  
Physical Dependence ◽  
Culture Technique ◽  
Morphine Sulfate ◽  
Morphine Dependence ◽  
Tissue Cultures ◽  
Culture Study ◽  
Low Concentrations

The addicting influence of morphine sulfate on the nervous tissue of new-born rabbits and puppies was studied by using the tissue culture technique. Certain low concentrations of morphine seemed to stimulate the growth of the cells as mitosis and proliferation increased. Nerve cells subjected to morphine sulfate developed increasing tolerance and physical dependence, which was tested through the processes of exposure to higher concentrations of morphine sulfate, withdrawal and reintroduction of this drug to the tissue cultures. Symbols in Figures MS: morphine sulfate DIV: days in vitro T.T.: number of test tubes RMS: reintroduction of morphine sulfate

A tissue culture technique for the assay of antibacterial immune sera

1971 ◽  
Vol 17 (1) ◽  
pp. 13-15 ◽  
Author(s):  
B. B. Diena ◽  
R. Wallace ◽  
C. P. Kenny ◽  
L. Greenberg
Keyword(s):  
Tissue Culture ◽  
Cell Cultures ◽  
Salmonella Typhi ◽  
Immune Serum ◽  
Culture Technique ◽  
Assay Method ◽  
Tissue Cultures ◽  
Significant Protection ◽  
Bacterial Challenge ◽  
Monkey Kidney Cell

A tissue culture assay method is described in which monkey kidney cell cultures were protected by antibacterial immune serum against infection by Salmonella typhi, Neisseria meningitidis, or Neisseria gonorrhoeae. The neutralization of the bacterial challenge was specific since neither heterologous nor normal sera gave significant protection to the tissue cultures.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay

1986 ◽  
Vol 81 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Marilda M. Siqueira ◽  
Vanja Ferreira ◽  
Jussara P. Nascimento
Keyword(s):  
Tissue Culture ◽  
Respiratory Syncytial Virus ◽  
Enzyme Immunoassay ◽  
Bacterial Contamination ◽  
Virus Isolation ◽  
Rapid Diagnosis ◽  
Tissue Cultures ◽  
Virus Diagnosis ◽  
Under Five ◽  
Syncytial Virus

Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been compared with virus isolaion in tissue culture for the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffering from acute respiratory iliness, during a period of six months from January to June 1982. Of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) were positive by immunofluorescence. The bacterial contamination of inoculated tissue cultures unfortunately prevented the isolation of virus from many samples. Specimens from 216 children were tested to compare enzyme immunoassay and immunofluorescence. Of these 60 (27%) were positive by EIA and 121 (56%) were positive by IFAT. Our results suggest that the EIA technique although highly specific is rather insensitive. This may be because by the time these tests were done the originl nasopharyngeal secretions were considerably diluted and contained more mucus fragments than the call suspension used for IFAT. Of the three techniques, IFAT gives the best results although EIA may be useful where IFAT is not possible.

scholarly journals Micro-operations on cells in tissue cultures

1931 ◽  
Vol 109 (763) ◽  
pp. 380-403 ◽  
Keyword(s):  
Tissue Culture ◽  
Continuous Control ◽  
Tissue Cultures ◽  
Special Paper ◽  
Long Time

The application of the micromanipulative technique to the study of cells in tissue culture has for a long time offered an interesting, though difficult, field of research. Thus far, comparatively little has been done, the most notable contributions being those of Levi and of Peterfi and co-workers (Levi, 1926 ; Peterfi and Olivo, 1925 ; Peterfi, 1927 ; Peterfi and Kapel, 1928). Peterfi has also written a special paper on the technique (1927). The main deterrent in this work has been the lack of sufficient ease in the accurate and continuous control of the microneedles under the conditions required.

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