NUTRITIONAL STUDIES WITHPSEUDOSARCOPHAGA AFFINIS(FALL.), A DIPTEROUS PARASITE OF THE SPRUCE BUD-WORM,CHORISTONEURA FUMIFERANA(CLEM.): IV. EFFECTS OF RIBONUCLEIC ACID, GLUTATHIONE, DEXTROSE, A SALT MIXTURE, CHOLESTEROL, AND FATS

1954 ◽  
Vol 32 (5) ◽  
pp. 358-365 ◽  
Author(s):  
H. L. House
Keyword(s):  
Ribonucleic Acid ◽  
Choristoneura Fumiferana ◽  
Optimum Growth ◽  
Salt Mixture ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Experimental Media ◽  
Nutritional Studies

The dipterous parasite Pseudosarcophaga affinis (Fall.) reared aseptically on chemically defined media requires ribonucleic acid, dextrose, a salt mixture, cholesterol, and fats for optimum growth; the differences between the results obtained on the experimental media and on the controls were statistically significant. Glutathione is not required for growth, but possibly aids in metamorphosis.

NUTRITIONAL STUDIES WITH PSEUDOSARCOPHAGA AFFINIS (FALL), A DIPTEROUS PARASITE OF THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (CLEM.): II. EFFECTS OF ELEVEN VITAMINS ON GROWTH

1954 ◽  
Vol 32 (5) ◽  
pp. 342-350 ◽  
Author(s):  
H. L. House
Keyword(s):  
Folic Acid ◽  
Growth And Development ◽  
Choristoneura Fumiferana ◽  
Choline Chloride ◽  
Larval Growth ◽  
Vitamin B ◽  
Calcium Pantothenate ◽  
Aminobenzoic Acid ◽  
Defined Media ◽  
Nutritional Studies

When the parasitic dipteron Pseudosarcophaga affinis (Fall.) was reared aseptically on chemically defined media; thiamine, riboflavin, calcium pantothenate, nicotinic acid, choline chloride, and biotin were essential for larval growth and development, but not vitamin B12, pyridoxine, folic acid, para-aminobenzoic acid, or inositol. Vitamin B12 had a slight beneficial effect on pupation, whereas the omission of para-aminobenzoic acid had a slight stimulatory effect on growth and development. The responses of larvae to deficiencies of different vitamins reached a decisive point at different stages of larval development.

NUTRITIONAL STUDIES WITH PSEUDOSARCOPHAGA AFFINIS (FALL.), A DIPTEROUS PARASITE OF THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (CLEM.): V. EFFECTS OF VARIOUS CONCENTRATIONS OF THE AMINO ACID MIXTURE, DEXTROSE, POTASSIUM ION, THE SALT MIXTURE, AND LARD ON GROWTH AND DEVELOPMENT; AND A SUBSTITUTE FOR LARD

1956 ◽  
Vol 34 (3) ◽  
pp. 182-189 ◽  
Author(s):  
H. L. House ◽  
J. S. Barlow
Keyword(s):  
Amino Acids ◽  
Growth And Development ◽  
Choristoneura Fumiferana ◽  
Amino Acid Mixture ◽  
Optimum Concentration ◽  
Acid Mixture ◽  
Salt Mixture ◽  
The Third ◽  
Time Required ◽  
Nutritional Studies

An improved chemically defined diet has been developed for aseptic rearing of the parasitic dipteron Pseudosarcophaga affiuis (Fall.). The time required for 50% of the larvae to develop to the third instar was decreased from nine to five days, and the percentage of larvae that became adults was increased from 19 to 60. These improvements resulted from a reduction in the dextrose concentration to 0.5% of the diet and the inclusion of 0.4% of lard or of a mixture of fatty acids. Reducing the concentration of the salt mixture when the concentration of potassium was increased did not affect growth. The optimum concentration of amino acids was about 2.0% of the medium. Excessive concentrations of amino acids, dextrose, and the salt mixture were toxic.

NUTRITIONAL STUDIES WITH PSEUDOSARCOPHAGA AFFINIS (FALL.), A DIPTEROUS PARASITE OF THE SPRUCE BUD-WORM, CHORISTONEURA FUMIFERANA (CLEM.): III. EFFECTS OF NINETEEN AMINO ACIDS ON GROWTH

1954 ◽  
Vol 32 (5) ◽  
pp. 351-357 ◽  
Author(s):  
H. L. House
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Growth And Development ◽  
Choristoneura Fumiferana ◽  
Larval Growth ◽  
First Instar ◽  
Defined Media ◽  
Nutritional Studies ◽  
Rates Of Growth ◽  
Number Of Individuals

Feeding tests with the parasite Pseudosarcophaga affinis (Fall.) reared aseptically on chemically defined media showed that food lacking l-arginine, l-histidine, dl-isoleucine, l-leucine, l-lysine, dl-methionine, dl-phenylalanine, dl-threonine, dl-tryptophane, or dl-valine failed to support larval growth beyond the first instar. Omitting glycine from the diet lowered the rate of growth and permitted only a small number of individuals to develop beyond the first instar. There were statistically significant differences between the rates of growth on the control diets and on diets lacking dl-alanine, glycine, dl-serine, or l-tyrosine. When dl-aspartic acid, l-cysteine, l-glutamic acid, l-hydroxyproline, or l-proline was omitted from the food, larval growth and development were not affected.

Cultivation and properties of Neisseria sp. grown in chemically defined media

1972 ◽  
Vol 18 (7) ◽  
pp. 1087-1090 ◽  
Author(s):  
C. P. Kenny ◽  
B. B. Diena ◽  
R. Wallace ◽  
L. Greenberg
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Present Report ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Specific Antisera

Neisseria Chemically Defined Medium (NCDM) has been used routinely in our laboratory for a variety of purposes. The present report describes the development of NCDM agar, wherein the NCDM base is sterilized by filtration and defined supplements and agar are added. The medium is transparent and both meningococci and gonococci grow within 72 h. When grown on NCDM agar, Types 2 and 3 gonococcal colonies tend to revert to Type 1. The serological grouping of meningococci with specific antisera is not affected by growth on this medium.Parallel investigations on the growth of these species in liquid NCDM demonstrated that the yield of Neisseria gonorrhoeae is enhanced when the medium is sterilized by filtration.

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