The effect of amino acids on the production of ochratoxin A in chemically defined media

1968 ◽  
Vol 34 (1) ◽  
pp. 433-440 ◽  
Author(s):  
N. P. Ferreira
Keyword(s):  
Amino Acids ◽  
Ochratoxin A ◽  
Chemically Defined Media ◽  
Defined Media

scholarly journals Development of Chemically Defined Media to Express Trp-Analog-Labeled Proteins in a Lactococcus lactis Trp Auxotroph

2016 ◽  
Vol 26 (4) ◽  
pp. 269-276
Author(s):  
Jinfeng Shao ◽  
Marcelo F.M. Marcondes ◽  
Vitor Oliveira ◽  
Jaap Broos
Keyword(s):  
Amino Acids ◽  
New Media ◽  
Lactococcus Lactis ◽  
Unnatural Amino Acids ◽  
Defined Medium ◽  
Trna Synthetase ◽  
Growth Media ◽  
Heterologous Proteins ◽  
Chemically Defined Media ◽  
Defined Media

Chemically defined media for growth of <i>Lactococcus lactis</i> strains contain about 50 components, making them laborious and expensive growth media. However, they are crucial for metabolism studies as well as for expression of heterologous proteins labeled with unnatural amino acids. In particular, the <i>L. lactis</i> Trp auxotroph PA1002, overexpressing the tryptophanyl tRNA synthetase enzyme of <i>L. lactis</i>, is very suitable for the biosynthetic incorporation of Trp analogs in proteins because of its most relaxed substrate specificity reported towards Trp analogs. Here we present two much simpler defined media for <i>L. lactis</i>, which consist of only 24 or 31 components, respectively, and with which the <i>L. lactis</i> Trp auxotroph shows similar growth characteristics as with a 50-component chemically defined medium. Importantly, the expression levels of two recombinant proteins used for evaluation were up to 2-3 times higher in these new media than in the 50-component medium, without affecting the Trp analog incorporation efficiency. Taken together, the simplest chemically defined media reported so far for <i>L. lactis</i> are presented. Since<i> L. lactis</i> also shows auxotrophy for Arg, His, Ile, Leu Val, and Met, our simplified media may also be useful for the biosynthetic incorporation of analogs of these five amino acids.

Production of amino acids by Rhizobium, Mesorhizobium and Sinorhizobium strains in chemically defined media

Amino Acids ◽  
2004 ◽  
Vol 27 (2) ◽  
pp. 169-174 ◽  
Author(s):  
V. Salmeron-Lopez ◽  
M. V. Martinez-Toledo ◽  
V. Salmeron-Miron ◽  
C. Pozo ◽  
J. Gonzalez-Lopez
Keyword(s):  
Amino Acids ◽  
Chemically Defined Media ◽  
Defined Media

scholarly journals Effect of methionine and cysteine deprivation on growth of different natural isolates of Lactobacillus spp. in chemically defined media

2008 ◽  
Vol 60 (4) ◽  
pp. 509-517 ◽  
Author(s):  
Jelena Lozo ◽  
Jelena Begovic ◽  
B. Jovcic ◽  
Natasa Golic ◽  
L. Topisirovic
Keyword(s):  
Amino Acids ◽  
Defined Medium ◽  
Ecological Niches ◽  
Chemically Defined Medium ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Natural Isolates ◽  
Lactobacillus Spp

The purpose of this study was to determine the ability of natural isolates of lactobacilli from different ecological niches to grow in a chemically defined medium in the presence or absence of sulphur-containing amino acids, methionine and/or cysteine. The obtained results indicate that cysteine is essential for growth of L. paracasei subsp. paracasei BGHN14 and BGSJ2-8, while methionine is essential for isolates BGHN40, BGCG31, and BGHV54T of the species L. plantarum. Methionine is also essential for growth of L. rhamnosus BGHV58T. Other analyzed strains, such as L. plantarum BGSJ3-18, BGZB19, BGHV52Ta, and BGHV43T, require the presence of both amino acids for their growth.

Growth of the flax rust Melampsora lini on chemically defined media

1973 ◽  
Vol 51 (1) ◽  
pp. 131-134 ◽  
Author(s):  
Franziska L. M. Turel
Keyword(s):  
Amino Acids ◽  
Trace Elements ◽  
Amino Acid ◽  
Mycelial Growth ◽  
Slow Growth ◽  
Essential Amino Acids ◽  
Chemically Defined Media ◽  
Melampsora Lini ◽  
Defined Media ◽  
Amino Acid Mixtures

Good saprobic growth of primary and established cultures of Melampsora lini (Ehrenb.) Lév., race 3, was obtained on chemically defined media containing modified Knop's minerals, various trace elements, 5% sucrose, and aspartic acid. Cysteine, cystine, and glutathione had to be added to the base medium singly or in combination. Alanine and methionine, added to minerals and sucrose, supported only sporadic and slow growth of primary flax rust cultures; and established cultures started on media containing more complex amino acid mixtures did not survive on the alanine–methionine medium. Types and concentrations of essential amino acids determined the rate of mycelial growth.

Chemically defined media for Pasteurella multocida and Pasteurella ureae, and a comparison of their thiamine requirements with those of Pasteurella haemolytica

1970 ◽  
Vol 16 (8) ◽  
pp. 751-757 ◽  
Author(s):  
G. E. Wessman ◽  
Geraldine Wessman
Keyword(s):  
Energy Source ◽  
Amino Acids ◽  
Pasteurella Multocida ◽  
Pasteurella Haemolytica ◽  
Phosphate Esters ◽  
Thiamine Pyrophosphate ◽  
Inorganic Salts ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Additional Amino Acid

Chemically defined media supported heavy cell yields of Pasteurella multocida and moderate yields of P. ureae (P. haemolytica var ureae). The medium for P. multocida consisted of 17 amino acids, plus inorganic salts, citrate, nicotinamide, pantothenate, thiamine or thiamine monophosphate, adenine, guanine, uracil, and energy source; the medium for P. ureae was the same except that it contained an additional amino acid. Six strains of P. multocida, five of P. ureae, and two of P. haemolytica grew well with normal amounts of thiamine; however, 9 of 11 strains of P. haemolytica could satisfy their thiamine requirement with free thiamine only if large amounts of the vitamin were provided. All strains used thiamine monophosphate or thiamine pyrophosphate efficiently. Pasteurella multocida grew well with a combination of the thiamine moieties, 4-methyl-5(β-hydroxyethyl)thiazole, and 2-methyl-4-amino-5-hydroxymethyl pyrimidine, or with their phosphate esters; P. haemolytica grew only if the phosphates were provided.

scholarly journals Enterococcus faecalis Grows on Ascorbic Acid

2013 ◽  
Vol 79 (15) ◽  
pp. 4756-4758 ◽  
Author(s):  
Ibrahim Mehmeti ◽  
Margrete Solheim ◽  
Ingolf F. Nes ◽  
Helge Holo
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Enterococcus Faecalis ◽  
Growth Yield ◽  
Content Type ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Fermentative Growth

ABSTRACTWe show thatEnterococcus faecaliscan utilize ascorbate for fermentative growth. In chemically defined media, growth yield was limited by the supply of amino acids, and the cells showed a much higher demand for amino acids than when they were grown on glucose.

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