Artificial maturation, fertilization, and early development of the American eel (Anguilla rostrata)

2010 ◽  
Vol 88 (11) ◽  
pp. 1121-1128 ◽  
Author(s):  
K. Oliveira ◽  
W. E. Hable
Keyword(s):  
Early Development ◽  
Anguilla Rostrata ◽  
Sargasso Sea ◽  
American Eel ◽  
Important Species ◽  
Vitro Fertilization ◽  
Commercially Important ◽  
Elusive Species ◽  
American Eels

Spawning for the American eel ( Anguilla rostrata (Le Sueur, 1817)) takes place in secretive locations within the Sargasso Sea, which has thus far prevented investigations of gametogenesis and early development in this ecologically and commercially important species. Attempts to induce maturation and reproduction in this species have been few and have produced limited results, with a single report of the production of gastrula-stage embryos. Here we report the successful maturation of female American eels. Maturation occurred within 13 weeks and ovulation was induced with a single injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP). Following in vitro fertilization, embryogenesis through hatching was observed and larvae were maintained for up to 6 days. We show that a crucial factor for successful fertilization is the stage of the oocyte at the time of induced ovulation. Oocytes that had not reached the migratory nucleus stage, or had passed this stage, were not successfully fertilized. These findings demonstrate that American eel can reproduce in the laboratory and previously untestable hypotheses pertaining to the developmental biology of this elusive species can now be explored.

Laboratory Experiments on the Orientation of the Adult American Eel, Anguilla rostrata

1968 ◽  
Vol 25 (10) ◽  
pp. 2143-2155 ◽  
Author(s):  
S. G. Miles
Keyword(s):  
Laboratory Experiments ◽  
Time Lapse ◽  
Physical Basis ◽  
Spawning Migration ◽  
Anguilla Rostrata ◽  
Sargasso Sea ◽  
Breeding Area ◽  
American Eel ◽  
American Eels

Adult American eels, Anguilla rostrata, caught on their spawning migration downriver, were filmed while in a circular tank, and the orientation of the eels was analysed by determining the direction of alignment of each eel in each frame of the time-lapse film. When allowed a view only of the sky, the eels oriented southward, in the direction of their supposed breeding area in the region of the Sargasso Sea. This southward orientation continued even if the eels were denied a view of the sky, provided they were subject to the diel rhythm of light–dark. Thus the eel seems capable of noncelestial orientation; the physical basis of this is not known.

Intracellular pH-temperature interactions of hepatocytes from American eels

1983 ◽  
Vol 245 (1) ◽  
pp. R32-R37
Author(s):  
P. J. Walsh ◽  
T. W. Moon
Keyword(s):  
Intracellular Ph ◽  
Acclimation Temperature ◽  
C Cells ◽  
Medium Ph ◽  
American Eel ◽  
Temperature Changes ◽  
American Eels ◽  
Temperature Interactions

The effects of acclimation temperature and acute temperature changes on the intracellular pH (pHi) of hepatocytes isolated from the American eel, Anguilla rostrata, were studied by the measurement of the distribution ratio of dimethyloxizolidinedione (DMO). Varying the concentration of DMO (10(-7) to 10(-4) M) did not affect estimates of pHi, indicating that DMO acts as an ideal pHi probe in eel hepatocytes. In vitro studies yielded values of liver cell pHi identical to those previously measured in vivo (in vitro pHi = 7.556 +/- 0.010; in vivo pHi = 7.570 +/- 0.049 at 20 degrees C); hepatocyte pHi varied inversely with acclimation temperature (5-20 degrees C) in a manner consistent with alphastat regulation (delta pH/delta T = -0.0182 +/- 0.021). During acute temperature increases (5-20 degrees C) and decreases (20-5 degrees C) hepatocytes regulated pHi to the appropriate (acclimated) value within 30-45 min posttransfer under conditions of constant medium pH (pHe). The effects of medium pH were also studied, and although patterns of pHi regulation differed between 5 and 20 degrees C cells, a pHi difference consistent with alphastat regulation was maintained between 5 and 20 degrees C cells over the pHe range 7.8-8.3.

scholarly journals Terrestrial Locomotion in American Eels (Anguilla rostrata): How Substrate and Incline Affect Movement Patterns

2020 ◽  
Vol 60 (1) ◽  
pp. 180-189
Author(s):  
Erica Redmann ◽  
Alina Sheikh ◽  
Areej Alqahtani ◽  
Mica McCarty-Glenn ◽  
Shazrah Syed ◽  
...  
Keyword(s):  
Wave Amplitude ◽  
Body Position ◽  
The Body ◽  
Complex Life Cycle ◽  
Anguilla Rostrata ◽  
American Eel ◽  
Distance Ratio ◽  
Terrestrial Environment ◽  
Terrestrial Locomotion ◽  
American Eels

Synopsis Fishes overcome a variety of challenges in order to invade the terrestrial environment. Terrestrial invasions by fish occur over a variety of environmental contexts. In order to advance their bodies on land, fishes capable of terrestrial excursions tend to use one of three different types of locomotor modes: axial-based, appendage-based, or axial-appendage-based. Elongate species with reduced appendages, such as the American eel, Anguilla rostrata, rely on axial based locomotion in water and on land. When eels move from water to land as part of their complex life cycle, they inevitably encounter a variety of substrates and must traverse variable degrees of incline. The aim of this study was to determine the effect of substrate and incline on the terrestrial locomotion of the American eel. In order to do this, eels were filmed from a dorsal view on three substrates and four inclines: sand, loose pebbles, and fixed (glued) pebbles at 0°, 5°, 10°, and 15°. We digitized 20 evenly spaced points along the body to examine the following characteristics of locomotion: velocity, distance ratio (DR), and wave parameters such as wave amplitude, frequency, and length and assessed whether substrate, incline, or body position affected these parameters. DR, our metric of movement efficiency, was highest on the flat sand condition and lowest on 15° pebble conditions. Efficiency also varied across the body. Velocity followed a similar pattern being highest on sand at 0° and lowest at the steepest inclines. Wave amplitude generally increased toward the tail but was similar across substrates and inclines. Wave frequency was relatively consistent across the body on both pebble substrates, but on sand, frequency was higher toward the head but decreased toward the tail. Wavelengths on sand were the longest at 0° near the head and shorter wavelengths were observed on steeper inclines. Both pebble substrates elicited lower wavelengths that were more similar across the body. Overall, A. rostrata were more effective in navigating compliant substrates but struggled at steeper inclines. Our findings provide insight into locomotor challenges that American eels may encounter as they move from and between bodies of water.

scholarly journals Early history of in vitro fertilization

Reproduction ◽  
2002 ◽  
pp. 181-196 ◽  
Author(s):  
BD Bavister
Keyword(s):  
In Vitro Fertilization ◽  
Early Development ◽  
Current Practice ◽  
Historical Review ◽  
Early History ◽  
Sperm Capacitation ◽  
Vitro Fertilization ◽  
History Of

Although in vitro fertilization (IVF) is used widely for a variety of purposes, it is often not appreciated how this technology was developed. A large number of experiments beginning in 1878 contributed to the first successful reports of IVF over 75 years later. The discovery of sperm capacitation in 1951 was central to the development of IVF technology, and it was rapidly followed by the first convincing reports of IVF in several species. The ability to fertilize oocytes in vitro has allowed major advances to be made into understanding the mechanisms involved in fertilization and early development, and IVF now supports reproductive biotechnology in animals and in humans. This article is a historical review of key experiments that helped to provide the basis for present day IVF procedures, placed into context with current practice.

The effect of propofol on parthenogenetic activation, in vitro fertilization and early development of mouse oocytes

1997 ◽  
Vol 67 (4) ◽  
pp. 769-774 ◽  
Author(s):  
Cécile Janssenswillen ◽  
Frank Christiaens ◽  
Frédéric Camu ◽  
André Van Steirteghem
Keyword(s):  
In Vitro Fertilization ◽  
Early Development ◽  
Vitro Fertilization ◽  
Parthenogenetic Activation ◽  
Mouse Oocytes

scholarly journals Effect of beta-mercaptoethanol during in vitro fertilization procedures on sperm penetration into porcine oocytes and the early development in vitro

Reproduction ◽  
2005 ◽  
Vol 130 (6) ◽  
pp. 889-898 ◽  
Author(s):  
Hiroaki Funahashi
Keyword(s):  
Early Development ◽  
Penetration Rate ◽  
Calcium Ionophore ◽  
Fluorescein Isothiocyanate ◽  
Cortical Granules ◽  
Pixel Intensity ◽  
Vitro Fertilization ◽  
Sperm Penetration ◽  
Development In Vitro

This study was carried out to determine the effects of beta-mercaptoethanol (bME) during a transient co-culture of gametes for 10 min, and/or the following culture until 6–9 h after insemination, on sperm penetration of porcine in vitro maturation (IVM) oocytes and the early development in vitro. When fresh spermatozoa were cultured in various concentrations of bME for 2 h, bME neutralized the stimulatory effect of caffeine-benzoate on sperm capacitation and the spontaneous acrosome reaction at 50–250 μmol/l. When 50 μmol/l bME were added during a transient co-culture of gametes for 10 min, the sperm penetration rate was reduced 9 h after insemination (70.5–82.0% vs 90.5–94.0% in the absence of bME), but the incidence of monospermic penetration was not affected. When 50 μmol/l bME were supplemented during culture after a transient co-culture, the sperm penetration rate was not affected, but the incidence of monospermy oocytes was increased (43.9–45.8% vs 31.7–34.3% in the absence of bME). The presence of bME following a transient co-culture minimized a decrease of oocyte glutathione content at 6 h after insemination (7.9 pmol/oocyte before in vitro fertilization (IVF), 6.7 pmol/oocyte in the presence of bME vs 5.5 pmol/oocyte in the absence of bME). When the distribution of cortical granules was evaluated 1 h after activation with calcium ionophore, mean pixel intensity of fluorescein isothiocyanate-labeled peanut agglutinin (FITC-PNA) at the cortex region was lower in the oocytes activated and cultured in the presence of 50 μmol/l bME. Although the presence of 50 μmol/l bME during a transient co-culture for 10 min and the following culture did not increased blastocyst formation (29.6–37.7%), 50 μmol/l bME during the following culture significantly increased the mean cell numbers per blastocyst (73.3–76.4 vs 51.2 in the presence and absence of bME respectively). These results demonstrate that supplementation with bME during IVF procedures, except during a transient co-culture period of gametes in the presence of caffeine, has a beneficial effect in maintaining the function of gametes, the incidence of normal fertilization and, consequently, the quality of IVF embryos.

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