Methods for determination of cell volume in tissue culture

1992 ◽  
Vol 70 (S1) ◽  
pp. S323-S333 ◽  
Author(s):  
H. K. Kimelberg ◽  
E. R. O'Connor ◽  
P. Sankar ◽  
C. Keese
Keyword(s):  
Tissue Culture ◽  
Cell Volume ◽  
Suspension Cultures ◽  
Coulter Counter ◽  
Dynamic Changes ◽  
Physical Methods ◽  
Monolayer Cultures ◽  
Primary Astrocyte

In this paper we present an overview of methods for determining cell volume in both suspension and monolayer cultures. Data from the use of selected methods such as the Coulter counter system for suspension cultures and radiolabelled intracellular markers for substratum-attached, monolayer cultures are presented. The advantages, limitations, and conditions under which the different methods can be used are discussed. It is pointed out that there is a need for more direct physical methods for measuring dynamic changes in the cell volume of monolayer cultures without removing the cells from the substratum. Data from a method applicable to such cultures that measures extracellular impedance are presented.Key words: cell volume, monolayer cultures, extracellular impedance, radiolabelled intracellular markers, primary astrocyte cultures.

scholarly journals Determination of bacterial cell volume with the Coulter Counter.

1986 ◽  
Vol 168 (3) ◽  
pp. 1466-1467 ◽  
Author(s):  
H E Kubitschek ◽  
J A Friske
Keyword(s):  
Cell Volume ◽  
Bacterial Cell ◽  
Coulter Counter

Electrical resistance method for measuring volume changes in monolayer cultures applied to primary astrocyte cultures

1993 ◽  
Vol 264 (2) ◽  
pp. C471-C478 ◽  
Author(s):  
E. R. O'Connor ◽  
H. K. Kimelberg ◽  
C. R. Keese ◽  
I. Giaever
Keyword(s):  
Cell Volume ◽  
Electrical Resistance ◽  
Cell Monolayer ◽  
Regulatory Volume Decrease ◽  
Neonatal Rat ◽  
Volume Changes ◽  
Resistance Method ◽  
Continuous Measurements ◽  
Monolayer Cultures ◽  
Primary Astrocyte

An electrical resistance method was developed to measure volume changes in substratum-attached monolayer cultures. Astrocytes in primary monolayer cultures prepared from neonatal rat cerebral cortex were placed in a confined channel containing a balanced salt solution, and the electrical resistance of the channel was measured using an applied alternating current. If the volume of the cells increases, then the volume of the solution within the channel available for current flow decreases by the same amount, resulting in an increase in the measured resistance through the channel. If the volume of the cells decreases, a decrease in resistance would be recorded. This method allows continuous measurements of volume changes in real time. When primary astrocyte monolayers were exposed to hyposmotic solutions (93-193 mosmol/kgH2O), they showed a rapid initial swelling and, in the continued presence of hyposmotic media, a characteristic regulatory volume decrease (RVD) in which there was a return to normal cell volume within approximately 20 min. Astrocytes exposed to hyperosmotic media (343-493 mosmol/kgH2O) gave a decrease in electrical resistance, indicating shrinkage. Putative endogenous effectors of astrocytic swelling, such as high extracellular K+ and glutamate, resulted in a much slower onset of swelling and no sign of RVD. This system can reliably measure the average change in cell monolayer volume to 1-2% and thus provides a sensitive means of continuous measurements of changes in cell volume in monolayer cultures.

Determination of circulating red blood cell volume with radioactive phosphorus

1949 ◽  
Vol 7 (2) ◽  
pp. 259
Author(s):  
R.T. Nieset ◽  
Blanche Porter ◽  
W.S. Trautman ◽  
Ralph M. Bell ◽  
William Parson ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Radioactive Phosphorus

scholarly journals Erythrocyte Antioxidative Enzymes in Experimentally Induced Osteopenia in Rats

2012 ◽  
Vol 56 (4) ◽  
pp. 669-675 ◽  
Author(s):  
Radosław P. Radzki ◽  
Marek Bieńko ◽  
Edyta Albera ◽  
Marta Kankofer
Keyword(s):  
Mineral Content ◽  
Mineral Density ◽  
Initial Body Weight ◽  
Dynamic Changes ◽  
Bone Parameters ◽  
Dynamic Relationship ◽  
Healthy Female ◽  
Female Wistar Rats ◽  
Determination Of Activity

Abstract The study aimed at the determination of dynamic relationship between mineralisation processes and antioxidative/oxidative status during the development of osteopenia. One hundred and two healthy female Wistar rats at the age of 2 months and initial body weight of 200 g were used in the experiment. The rats were divided into control (CON, n=6), sham operated (SHO, n=48), and ovariectomised (OVX, n=48) groups. Animals from SHO (n=6) and OVX (n=6) groups were sacrificed every week during 8 weeks of the experiment in order to detect dynamic changes in examined parameters. The samples were collected weekly from day 7 to day 56. The femora were examined with the use of DXA (bone mineral density) and pQCT (area, mineral content, volumetric density of trabecular and cortical part of distal femora). The pQCT scans were performed 5 mm from distal end of the tibia. The determination of activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in haemolysates of erythrocytes were performed spectrophotometrically. Obtained data showed wave like changes in both enzyme activities and bone parameters and indicated the importance of the 2nd-3rd and 5th-6th week after surgery as a key moment for bone metabolism and activity of enzymatic antioxidative defence during the development of osteopenia induced by bilateral ovariectomy. The obtained results proved that alterations in activity of GSH-Px and SOD, and pQCT ahead the changes registered by DXA by 7 d.

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

Sign in / Sign up

Export Citation Format

Share Document