scholarly journals Determination of organic structures by physical methods (Nachod, F. C.; Phillips, W. D.; eds.)

1962 ◽  
Vol 39 (9) ◽  
pp. 487
Author(s):  
Frederick G. Bordwell
Keyword(s):  
Physical Methods

Methods for determination of cell volume in tissue culture

1992 ◽  
Vol 70 (S1) ◽  
pp. S323-S333 ◽  
Author(s):  
H. K. Kimelberg ◽  
E. R. O'Connor ◽  
P. Sankar ◽  
C. Keese
Keyword(s):  
Tissue Culture ◽  
Cell Volume ◽  
Suspension Cultures ◽  
Coulter Counter ◽  
Dynamic Changes ◽  
Physical Methods ◽  
Monolayer Cultures ◽  
Primary Astrocyte

In this paper we present an overview of methods for determining cell volume in both suspension and monolayer cultures. Data from the use of selected methods such as the Coulter counter system for suspension cultures and radiolabelled intracellular markers for substratum-attached, monolayer cultures are presented. The advantages, limitations, and conditions under which the different methods can be used are discussed. It is pointed out that there is a need for more direct physical methods for measuring dynamic changes in the cell volume of monolayer cultures without removing the cells from the substratum. Data from a method applicable to such cultures that measures extracellular impedance are presented.Key words: cell volume, monolayer cultures, extracellular impedance, radiolabelled intracellular markers, primary astrocyte cultures.

scholarly journals THE SCHEMATIZATION FOR TIDAL COMPUTATIONS IN CASE OF VARIABLE BOTTOM SHAPE

1972 ◽  
Vol 1 (13) ◽  
pp. 132
Author(s):  
J.J. Dronkers
Keyword(s):  
Coefficient Of Friction ◽  
Coastal Waters ◽  
Physical Models ◽  
Practical Case ◽  
Two Dimensional ◽  
Physical Methods ◽  
Variable Bottom ◽  
The Coefficient Of Friction ◽  
Shallow Coastal Waters

Mathematical and physical methods can be applied for tidal studies. After general considerations on these methods, some practical aspects of tidal computations are discussed, in particular the schematization for tidal computations in case of variable bottom shape in shallow coastal waters. The relation with the coefficient of friction is dealt with. A combined one- and two dimensional tidal computation is considered. Also an example is given of the determination of the coefficient of friction in a very shallow region; the variations, which are found in this practical case are discussed. 1. General considerations on the application of mathematical and physical methods for tidal studies. Tidal problems may be solved by means of mathematical or physical models. Both kinds of models are approximations of the reality; in some respects in a different way.

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