Effect of nickel chloride on streptozotocin-induced diabetes in rats

1988 ◽  
Vol 66 (5) ◽  
pp. 663-665 ◽  
Author(s):  
Ethel L. B. Novelli ◽  
Ney L. Rodrigues ◽  
Bartolomé O. Ribas
Keyword(s):  
Superoxide Dismutase ◽  
Body Weight ◽  
Single Dose ◽  
Protective Effect ◽  
Plasma Insulin ◽  
Nickel Chloride ◽  
Superoxide Dismutase Activity ◽  
Streptozotocin Induced Diabetes

The potential of nickel chloride to prevent streptozotocin-induced hyperglycemia was tested in rats in vivo. To induce diabetes, streptozotocin (100 mg/kg body weight) was injected as a single dose. Streptozotocin treatment resulted in a significant decrease in plasma insulin and ceruloplasmin, and pancreatic Cu, protein, and Cu–Zn superoxide dismutase activity. In rats treated with nickel chloride (10 mg/kg body weight) and streptozotocin, these values were comparable with those observed in control rats. The results indicate that nickel chloride injected before streptozotocin prevented streptozotocin-induced hyperglycemia, and suggest that the protective effect was related to Cu–Zn superoxide dismutase activity, mediated by copper.

scholarly journals Two New Compounds From the Heartwood of Dalbergia melanoxylon and Their Protective Effect on Hypoxia/Reoxygenation Injury in H9c2

2021 ◽  
Vol 16 (1) ◽  
pp. 1934578X2098777
Author(s):  
Yang Liu ◽  
Ni Zhang ◽  
Jun-wei He ◽  
Lan-ying Chen ◽  
Li Yang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Lactate Dehydrogenase ◽  
Protective Effect ◽  
Superoxide Dismutase Activity ◽  
Spectral Evidence ◽  
Reoxygenation Injury ◽  
Dalbergia Melanoxylon ◽  
New Compounds ◽  
Hypoxia Reoxygenation

A new neoflavonoid, named as (7 R)-(-)-3′,5-dihydroxy-4′,2,4-trimethoxy-dalbergiquinol (1) and a new phenanthrenedione, named as 3′,7-dihydroxy-3,6- dimethoxy-9-phenyl-1,4-phenanthrenedione (2), together with 4 known compounds, 5- O-methyldalbergiphenol (3), 3′,7-dihydroxy-4′,3,6-trimethoxy-9-phenyl-1,4-phenanthrenedione (4), (+)-obtusafuran (5), and melanoxin (6) were isolated from the heartwood of Dalbergia melanoxylon. Their structures were elucidated on the basis of chemical and spectral evidence, as well as by comparison with literature data. Moreover, compound 1 showed a protective effect on hypoxia/reoxygenation injury in H9c2 at 10.0 μM by decreasing lactate dehydrogenase and malondialdehyde activity and enhancing superoxide dismutase activity.

In Vivo Effect of Heparin on Acid and Alkaline Ribonuclease Activities of Mouse Liver

1956 ◽  
Vol 184 (2) ◽  
pp. 415-417 ◽  
Author(s):  
Gaston De Lamirande ◽  
George Weber ◽  
Antonio Cantero
Keyword(s):  
Tissue Culture ◽  
Body Weight ◽  
Single Dose ◽  
Ribonucleic Acid ◽  
Mouse Liver ◽  
Single Injection ◽  
Coagulation Time ◽  
Alkaline Ribonuclease ◽  
Blood Coagulability

A single dose of 30 µg/gm body weight of depo-heparin was injected subcutaneously into white Swiss mice. At 1, 3, 6 and 12 hours after the injection, the blood coagulation time was measured and the activity of acid and alkaline ribonuclease of liver was determined. This single injection of depo-heparin significantly inhibited the acid and alkaline ribonucleases of liver 1 hour after injection. The enzymatic activities significantly increased after the blood coagulability was restored. The in vivo inhibition of acid and alkaline ribonuclease activity supports the explanation that the accumulation of ribonucleic acid in cells of tissue culture in the presence of heparin might be due to the inhibition of ribonuclease.

scholarly journals Activity of Tigecycline (GAR-936), a Novel Glycylcycline, against Enterococci in the Mouse Peritonitis Model

2003 ◽  
Vol 47 (2) ◽  
pp. 529-532 ◽  
Author(s):  
Esteban C. Nannini ◽  
Suresh R. Pai ◽  
Kavindra V. Singh ◽  
Barbara E. Murray
Keyword(s):  
Body Weight ◽  
Mouse Model ◽  
Protective Effect ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Tetracycline Resistance ◽  
Resistance Determinant ◽  
Subcutaneous Dose ◽  
Peritonitis Model

ABSTRACT A novel glycylcycline agent, tigecycline (GAR-936), was evaluated in vivo in the mouse model of peritonitis against three Enterococcus faecalis and four Enterococcus faecium isolates with different susceptibilities to vancomycin and tetracyclines, all of which were inhibited by ≤0.125 μg of tigecycline/ml. Using a single subcutaneous dose, tigecycline displayed a protective effect (50% protective dose, ≤5.7 mg/kg of body weight) against all strains tested, including two with Tn925 (from the Tn916 family), which contains the Tet(M) tetracycline resistance determinant, as well as VanA and VanB strains. As expected, tetracycline and minocycline were ineffective against the isolates carrying Tn925.

Differential Contribution of Superoxide Dismutase Activity by Prion Protein in Vivo

2000 ◽  
Vol 273 (1) ◽  
pp. 136-139 ◽  
Author(s):  
Boon-Seng Wong ◽  
Tao Pan ◽  
Tong Liu ◽  
Ruliang Li ◽  
Pierluigi Gambetti ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Prion Protein ◽  
Superoxide Dismutase Activity ◽  
Differential Contribution

scholarly journals In VivoAntioxidant Activity of Deacetylasperulosidic Acid in Noni

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
De-Lu Ma ◽  
Mai Chen ◽  
Chen X. Su ◽  
Brett J. West
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Superoxide Dismutase Activity ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dose Dependent

Deacetylasperulosidic acid (DAA) is a major phytochemical constituent ofMorinda citrifolia(noni) fruit. Noni juice has demonstrated antioxidant activityin vivoand in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group), 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activityin vivobut additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

scholarly journals Effect of Quercetin in the 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-Induced Mouse Model of Parkinson's Disease

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Chuanfeng Lv ◽  
Tie Hong ◽  
Zhen Yang ◽  
Yu Zhang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Superoxide Dismutase ◽  
Body Weight ◽  
Glutathione Peroxidase ◽  
Mouse Model ◽  
Protective Effect ◽  
Mechanisms Of Action ◽  
Quercetin Treatment

In this paper, the protective effect of the bioflavonoid quercetin on behaviors, antioxidases, and neurotransmitters in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-(MPTP-) induced Parkinson's disease (PD) was investigated. Quercetin treatment (50 mg/kg, 100 mg/kg and 200 mg/kg body weight) was orally administered for 14 consecutive days. The results show that quercetin treatment markedly improves the motor balance and coordination of MPTP-treated mice. Significant increases were observed in the activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), and Na+, K+-ATPase, AchE, the content of dopamine (DA) in the quercetin plus MPTP groups compared to those in the MPTP group. Significant reduction the 4-hydroxy-2-nonenal (4-HNE) immunoreactivity in striatum of brains was observed in the quercetin plus MPTP groups in comparison to the MPTP group. Taken together, we propose that quercetin has shown antiparkinsonian properties in our studies. More work is needed to explore detailed mechanisms of action.

In-vitro and in-vivo responses to chicken LHRH-I and chicken LHRH-II in male turkeys (Meleagris gallopavo)

1992 ◽  
Vol 132 (3) ◽  
pp. 387-393 ◽  
Author(s):  
D. Guémené ◽  
J. B. Williams
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Meleagris Gallopavo ◽  
Initial Increase ◽  
Prolonged Action ◽  
The Difference ◽  
High Doses ◽  
Stimulation Of

ABSTRACT Stimulation of male turkey hypophyses in vitro with chicken (c)LHRH-I, cLHRH-II or porcine (p)LHRH (0·1 μmol/l) using a perifusion technique caused an increase in the release of LH. In this system, cLHRH-II was approximately 2·5-fold more potent than cLHRH-I and pLHRH which were equipotent. The difference was due to a greater amplitude of the response but not to a prolonged action. Hypophyseal desensitization to a subsequent stimulation was induced when the interval between stimulations was 30 min, but did not occur when lengthened to 60 or 120 min. Injection of a single dose of cLHRH-I or -II in vivo at doses of 10 and 0·1 nmol/kg body weight stimulated increases in the plasma concentration of LH and testosterone initiated within 1 or 10 min after injection respectively. As in vitro, cLHRH-II induced greater responses, which were dose-related, than did cLHRH-I. However, this difference could be attributed to a greater potency of cLHRH-II and to a more prolonged action. At the 10 nmol/kg dose, the shape of the LH response to cLHRH-II changed; it consisted of an initial increase during 10 min after injection, followed by a more sustained phase during which LH levels were still increasing between 20 and 60 min after injection. In contrast, after an injection of cLHRH-I at doses of 10 or 0·1 nmol/kg or cLHRH-II at a dose of 0·1 nmol/kg, LH levels were at a peak within 5 min and thereafter declined gradually. This decrease in LH may therefore simply be related to the disappearance of the LHRH from the circulation or to other unknown actions of cLHRH-II, when high doses are used. Journal of Endocrinology (1992) 132, 387–393

Chronic UVB irradiation induces superoxide dismutase activity in human epidermis in vivo

1995 ◽  
Vol 30 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Kari Punnonen ◽  
Kirsi Lehtola ◽  
Pekka Autio ◽  
Urpo Kiistala ◽  
Markku Ahotupa
Keyword(s):  
Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
Human Epidermis ◽  
Uvb Irradiation

scholarly journals Efficacies of Vesicular and Free Sodium Stibogluconate Formulations against Clinical Isolates ofLeishmania donovani

2001 ◽  
Vol 45 (12) ◽  
pp. 3555-3559 ◽  
Author(s):  
K. C. Carter ◽  
A. B. Mullen ◽  
S. Sundar ◽  
R. T. Kenney
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Single Dose ◽  
Leishmania Donovani ◽  
Laboratory Strain ◽  
Free Drug ◽  
Clinical Isolates ◽  
Sodium Stibogluconate

ABSTRACT In this study, the in vitro and in vivo efficacies of free sodium stibogluconate (SSG) and a nonionic surfactant vesicular formulation of SSG (SSG-NIV) against a laboratory strain ofLeishmania donovani (MHOM/ET/67:LV82) and different clinical isolates of L. donovani were determined. Treatment with SSG-NIV was more effective against intramacrophage amastigotes than treatment with SSG. In vivo murine studies showed that there was interstrain variability in the infectivity of the different L. donovani strains, with two of the strains (20001 and 20003) giving low parasite burdens. In addition, interstrain variability in the antileishmanial efficacy of SSG in a single dose containing 300 mg of Sb(V)/kg of body weight was observed. This dose of free drug either caused a >97% reduction in liver parasite burdens or had no significant effect on parasite burdens compared with the result with the respective control. In some instances, treatment with this free SSG dose also caused a significant reduction in spleen (strain 20006) or bone marrow (strains 20001 and 20009) parasite burdens. Treatment with SSG-NIV was more effective than that with SSG against all of the strains tested. In SSG-responsive strains, the reduction in liver parasite burdens by SSG-NIV treatment was similar to that caused by free SSG. In SSG-nonresponsive strains, SSG-NIV treatment caused at least a 95% reduction in liver parasite burdens. Overall, these results indicate that the use of a vesicular formulation of SSG is likely to increase its clinical efficacy against visceral leishmaniasis.

In vivo oral efficacy of levofloxacin for treatment of systemic Pseudomonas aeruginosa infections in a murine model of septicemia.

1996 ◽  
Vol 40 (12) ◽  
pp. 2894-2897 ◽  
Author(s):  
S K Yagel ◽  
J F Barrett ◽  
D J Amaratunga ◽  
M B Frosco
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Body Weight ◽  
Single Dose ◽  
Divided Dose ◽  
Murine Model ◽  
Total Daily Dose ◽  
Dosing Regimen ◽  
Daily Dose ◽  
Effective Doses

The in vivo efficacies of levofloxacin and ciprofloxacin in lethal, systemic Pseudomonas aeruginosa infections in mice were compared. MICs of levofloxacin and ciprofloxacin ranged from 0.5 to 2.0 micrograms/ml and from 0.12 to 1.0 microgram/ml respectively. Infecting doses ranged from 5.0 x 10(1) to 3.2 x 10(3) CFU per mouse, depending on the isolate. Test fluoroquinolones were administered orally at 1 h (single dose) or at 1 and 3 h (divided dose) postinfection, with 10 infected mice used for each of six concentrations of each fluoroquinolone tested (1 to 40 mg/kg of body weight) in each dosing regimen. Whether given in a single or a divided dose, the total daily dose was the same for each fluoroquinolone. For mice treated 1 h postinfection with levofloxacin and ciprofloxacin, the effective doses for 50% of the infected mice ranged from 2.09 to 13.80 mg/kg and from 2.34 to 11.22 mg/kg, respectively, and for those treated 1 and 3 h postinfection, the effective doses for 50% of the infected mice ranged from 3.71 to 16.98 mg/kg and from 2.95 to 13.18 mg/kg, respectively. Although the potency varied for both levofloxacin and ciprofloxacin among all strains of P. aeruginosa tested, there were small differences within the same strain for levofloxacin and ciprofloxacin when given in the same dosing regimen. Levofloxacin proved nearly as effective as ciprofloxacin against a systemic P. aeruginosa infection in mice.

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