Potentiation of salivary fluid secretion in ixodid ticks: a new receptor system for γ-aminobutyric acid

1986 ◽  
Vol 64 (8) ◽  
pp. 1119-1126 ◽  
Author(s):  
Paul J. Lindsay ◽  
W. Reuben Kaufman
Keyword(s):  
Salivary Glands ◽  
Fluid Secretion ◽  
Recognition Site ◽  
Ixodid Ticks ◽  
Aminobutyric Acid ◽  
Intrinsic Activity ◽  
Receptor System ◽  
Gaba Antagonists ◽  
Tick Salivary Glands

γ-Aminobutyric acid (GABA), having minimal intrinsic activity, potentiates dopamine-induced fluid secretion in salivary glands of female ixodid ticks. Because the effect of GABA was similar to that of spiperone, we tested whether these two drugs act at a common recognition site. Potentiation was not augmented when salivary glands were exposed to supramaximal concentrations of spiperone (1 μM) plus GABA (100 μM). (±)-Sulpiride (100 μM), a spiperone antagonist in this system, also blocked GABA-induced potentiation. Picrotoxin (100 μM) and (−)-bicuculline (100 μM), two GABA antagonists, blocked GABA-induced and spiperone-induced potentiation. Inhibition of GABA by picrotoxin and (−)-bicuculline was noncompetitive. Muscimol (an agonist at GABAA receptors) also potentiated dopamine-induced secretion. Baclofen (an agonist at GABAB receptors) did not elicit potentiation. We suggest that GABA may function as a neuromodulator for dopamine-induced fluid secretion in tick salivary glands.

Fluid secretion by isolated tick salivary glands dependent on an intact cytoskeleton

1994 ◽  
Vol 24 (4) ◽  
pp. 563-567 ◽  
Author(s):  
William J. Lamoreaux ◽  
Glen R. Needham ◽  
Lewis B. Coons
Keyword(s):  
Salivary Glands ◽  
Fluid Secretion ◽  
Tick Salivary Glands

The influence of various factors on fluid secretion by in vitro salivary glands of ixodid Ticks

1976 ◽  
Vol 64 (3) ◽  
pp. 727-742
Author(s):  
W. Kaufman
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Culture Medium ◽  
Dry Weight ◽  
Fluid Secretion ◽  
Ixodid Tick ◽  
Ixodid Ticks ◽  
Mammalian Tissue ◽  
Stimulating Factor

1. Salivary glands of the female ixodid tick, Dermacentor andersoni, secrete fluid in vitro when bathed in a slightly modified version of the mammalian tissue culture medium ‘TC 199′. 2. Rate of salivation in vitro increases with progression of feeding, but there is no comparable increase in dry weight of the salivary glands during the early phase of engorgement. Engorged ticks secreted at only 25% the rate of 90–250 mg ticks, indicating that salivary gland degeneration has already begun in the very early post-engorgement stage. 3. A salivary gland stimulating factor can be detected in the nervous system but not in other tissues. 4. Male salivary glands secrete at only 1/20th the rate of female glands. Thus males probably do not use their salivary glands as osmoregulatory organs. 5. From the uniform lack of response to ACh and uniform response to DA in 7 ixodid tick species, it is suggested that the control of salivation is similar throughout the ixodid family.

The effect of avermectins on feeding, salivary fluid secretion, and fecundity in some ixodid ticks

1986 ◽  
Vol 2 (1) ◽  
pp. 1-18 ◽  
Author(s):  
W. Reuben Kaufman ◽  
Sandra G. Ungarian ◽  
Alexandra E. Noga
Keyword(s):  
Fluid Secretion ◽  
Ixodid Ticks

Salivary glands in ixodid ticks: control and mechanism of secretion

2000 ◽  
Vol 46 (7) ◽  
pp. 1069-1078 ◽  
Author(s):  
J.R Sauer ◽  
R.C Essenberg ◽  
A.S Bowman
Keyword(s):  
Salivary Glands ◽  
Ixodid Ticks

Studies on the mechanism of fluid secretion by isolated salivary glands of Calliphora

1976 ◽  
Vol 64 (2) ◽  
pp. 311-322
Author(s):  
M. J. Berridge ◽  
B. D. Lindley ◽  
W. T. Prince
Keyword(s):  
Salivary Glands ◽  
Apical Membrane ◽  
Potassium Concentration ◽  
Basal Membrane ◽  
Fluid Secretion ◽  
Sodium Permeability ◽  
Bathing Medium ◽  
Major Cation ◽  
Diuretic Agent ◽  
External Potassium

1. Potassium is the major cation in the secretion of the salivary glands of Calliphora and is necessary for full secretory rates. 2. Other ions (rubidium and sodium) can support secretion in the absence of potassium. 39. During stimulation with 5-HT a Nernst plot of the basal membrane potential has a slope of 53 mV for a tenfold change in external potassium concentration and the slope at rest deviates from this over the range I-20 mM external potassium. 4. Hyperpolarization of the basal membrane by 5-HT is abolished if the chloride in the bathing medium is replaced by isethionate. 5. The diuretic agent amiloride inhibits fluid secretion by a mechanism which may include a reduction in calcium entry in addition to its recognized effect on sodium permeability. 6. A model is proposed in which fluid secretion is driven by the active transport of potassium across the apical membrane with chloride following passively.

scholarly journals Salp25D, anIxodes scapularisAntioxidant, Is 1 of 14 Immunodominant Antigens in Engorged Tick Salivary Glands

2001 ◽  
Vol 184 (8) ◽  
pp. 1056-1064 ◽  
Author(s):  
Subrata Das ◽  
Gautam Banerjee ◽  
Kathleen DePonte ◽  
Nancy Marcantonio ◽  
Fred S. Kantor ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Immunodominant Antigens ◽  
Tick Salivary Glands
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