Induction of superovulation in mature rats with gonadotrophins

1970 ◽  
Vol 48 (3) ◽  
pp. 196-199 ◽  
Author(s):  
S. M. Husain ◽  
R. Saucier
Keyword(s):  
Mature Female ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
High Doses ◽  
Pregnant Mare Serum Gonadotrophin

Ovulation was induced in mature female rats by appropriately timed injections of pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG). Various combinations of the gonadotrophins were used. Maximal ovulatory response was consistently obtained with 50 I.U. PMSG and any combination of HCG between 50 I.U. and 5000 I.U. On the basis of the results obtained it is recommended that 50 I.U. PMSG and 50 I.U. HCG be injected to obtain a maximal ovulatory response in mature rats. High doses of PMSG (but not HCG) suppress ovulation markedly.

Internalization of receptor-bound human chorionic gonadotrophin in preovulatory rat granulosa cells in vivo

1983 ◽  
Vol 103 (3) ◽  
pp. 406-412 ◽  
Author(s):  
Kalle Jääkeläinen ◽  
Seppo Markkanen ◽  
Hannu Rajaniemi
Keyword(s):  
Plasma Membrane ◽  
Granulosa Cells ◽  
Subcellular Distribution ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Electron Microscope Autoradiography ◽  
Silver Grains ◽  
Pregnant Mare Serum Gonadotrophin

Abstract. The subcellular distribution of 125I-labelled human chorionic gonadotrophin (hCG) in preovulatory rat granulosa cells was studied in vivo. Pregnant mare serum gonadotrophin-pretreated immature female rats received an iv injection of [125I]hCG a few hours before the endogenous preovulatory gonadotrophin surge. The animals were killed at 2 or 6 h after the [125I]hCG injections. Light microscope autoradiographs showed that the mural granulosa cells of large follicles were the most highly labelled cells in the ovaries. Electron microscope autoradiography was used to study the subcellular distribution of radioactivity in the mural granulosa cells. At 2 h 45% of the counted silver grains were associated with the plasma membrane and 10% with the lysosomes, at 6 h the values were 51% and 9%, respectively. The distribution of the observed silver grains was compared with the generated expected source to grain pairs by computerized linear multiple regression analysis. The magnitudes of the regression coefficients revealed that the plasma membrane and the lysosomes were the only specifically 125I-labelled organelles, that a few radioactive molecules were located diffusely over the cytoplasm at 2 h and that the 125I-radioactivity of the nuclei was negligible. The present results suggest that preovulatory rat granulosa cells are in vivo able to internalize into lysosomes [125I]hCG initially bound to LH/hCG receptors of the plasma membrane.

DIE BEDEUTUNG VON GONADEN UND HYPOPHYSE FÜR DIE WIRKUNG GONADOTROPER HORMONE AUF DIE SCHILDDRÜSENFUNKTION

1960 ◽  
Vol XXXIV (II) ◽  
pp. 176-188 ◽  
Author(s):  
A. Hasselblatt ◽  
Ch. Ratabongs
Keyword(s):  
Thyroid Gland ◽  
Pituitary Gland ◽  
Thyroid Function ◽  
Functional Relationship ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Male Rats ◽  
Hormonal Secretion ◽  
Pregnant Mare Serum Gonadotrophin

ABSTRACT The effect of pregnant mare serum gonadotrophin (PMS) and human chorionic gonadotrophin (HCG) on the thyroid gland of normal, of gonadectomized and of hypophysectomized infantile rats has been studied. Gonadotrophin treatment stimulated the thyroid of normal and hypophysectomized female rats. A corresponding effect was not observed in gonadectomized female or in normal and gonadectomized male rats. These results show that the gonadotrophic hormones stimulate thyroid function indirectly by increasing the hormonal secretion of the ovaries. An intimate functional relationship between the ovaries and the thyroid gland was thus demonstrated. As the stimulating effect of gonadotrophin treatment was also present in hypophysectomized female rats, it was concluded that the oestrogens act directly on the thyroid gland. Their thyrotrophic action is not mediated by the pituitary gland.

THE EFFECT OF CHLORMADINONE ON THE RESPONSE OF THE OVARIES AND UTERUS OF THE IMMATURE RAT TO GONADOTROPHIC STIMULATION

1964 ◽  
Vol 30 (2) ◽  
pp. 235-245 ◽  
Author(s):  
M. J. K. HARPER
Keyword(s):  
Ovarian Response ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Corpora Lutea ◽  
Immature Female ◽  
Immature Rat ◽  
Orally Active ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Intact Rats

SUMMARY The effects of chlormadinone (6-chloro-Δ6-17α-acetoxyprogesterone), an orally active progestational agent without significant oestrogenic activity, on the response of the ovaries of intact and hypophysectomized immature female rats to exogenous gonadotrophin have been examined. Administration of the steroid whether starting on the same day as, or 4 days before treatment with gonadotrophin, did not depress the ovarian response in intact rats. In hypophysectomized animals, pretreated with the progestagen, the ovarian response to gonadotrophin was depressed. In intact rats, treatment with the steroid and pregnant mare serum gonadotrophin (PMSG) resulted in ovulation, whereas in similar animals given PMSG alone no corpora lutea were found. Corpora lutea were seen in all groups given PMSG and human chorionic gonadotrophin (HCG) but ovulation occurred earlier when, in addition, treatment with the steroid was included. In only one experiment with intact rats did administration of the steroid alone cause a significant increase in uterine weight compared with controls. In neither experiment on hypophysectomized animals did such an increase occur, and significant decreases were recorded.

scholarly journals Kondisi Neonatus Anak Babi yang Dilahirkan oleh Induk yang Disuntik Gonadotropin Sebelum Pengawinan (THE NEONATE CONDITIONS OF PIGLETS BORN FROM SOW INJECTED WITH GONADOTROPIN PRIOR TO MATING)

2018 ◽  
Vol 19 (1) ◽  
pp. 76
Author(s):  
Friska Mery Montolalu ◽  
Anita Esfandiari ◽  
Damiana Rita Ekastuti ◽  
Wasmen Manalu
Keyword(s):  
Mature Female ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Nacl Solution ◽  
Birth Process ◽  
Prenatal Growth ◽  
Management Condition ◽  
Uterine Environment ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Experimental Pigs

Injections of sows with pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) prior to mating have been shown to improve endogenous secretions of pregnant hormones that improve and optimize the uterine environment and condition during pregnancy that eventually lead to give birth to superior piglets. This experiment was designed to study the effects of PMSG and hCG injections of sows prior to mating on prenatal growth as indicated by the conditions of the the neonate piglets. Thirty mature female Landrace pigs were divided into 2 groups i.e., 15 pigs were injected with PMSG and hCG prior to mating, and the other 15 pigs were injected with physiological NaCl solution as a control. Before mating, the estrus cycles of the experimental sows were synchronized by injecting prostaglandin two times with 14 days interval. The experimental pigs were injected with PMSG and hCG or 0.90% NaCl solution at the same time with the second prostaglandin injection and were further mated naturally. During pregnancy, the experimental sows were maintained with the normal management condition. The results showed that piglest born to sows injected with PMSG and hCG prior to mating had shorter and faster birth process with higher activities and survivals as compared to those born to control sows. Piglets born by sow that were injected with PMSG and hCG before mating had a more optimum birth weight accompanied by greater body length, limb height, and higher rear limb height. Piglest born to by sows that were injected with PMSG and hCG prior to mating showed superior conditions that would contribute to optimum and superior preweaning and postweaning growths until maturity.

OVULATION INDUCED BY PREGNANT MARE SERUM GONADOTROPHIN IN THE IMMATURE RAT TREATED NEONATALLY WITH A LOW OR A HIGH DOSE OF ANDROGEN

1972 ◽  
Vol 55 (3) ◽  
pp. 533-541 ◽  
Author(s):  
J. Th. J. UILENBROEK ◽  
J. J. van der WERFF ten BOSCH
Keyword(s):  
Testosterone Propionate ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
High Dose ◽  
Immature Female ◽  
Immature Rat ◽  
Progesterone Treatment ◽  
Combined Administration ◽  
Pregnant Mare Serum Gonadotrophin

SUMMARY Ovulation-inducing effects of pregnant mare serum gonadotrophin (PMSG) were studied in immature female rats treated on day 5 (day 1 = day of birth) with oil or with 5 or 1250 μg testosterone propionate (TP). The response of rats treated with 1250 μg TP was negligible regardless of the age of the animals and of the dose of PMSG. The response of rats treated with 5 μg TP to PMSG alone was low (36% of rats, with 2·6 ova/ovulating rat), but could be improved by progesterone administration 2 days after PMSG injection (91% of rats, with 14·5 ova/ovulating rat). At every age and dose of PMSG tested the response of animals treated with 5 μg TP to combined PMSG and progesterone treatment was less than that of control animals. It is concluded that neonatal TP treatment diminishes the release of endogenous ovulating hormone subsequent to PMSG injection. This effect is dependent on the dose of TP used, but already demonstrable in animals treated with 5 μg TP on day 5, which would have been cyclic and fertile after puberty. Only for the animals treated with 1250 μg TP could a decreased sensitivity of the ovaries to combined administration of PMSG and human chorionic gonadotrophin be demonstrated.

Oestrus and ovulation following administration of placental gonadotrophins to Merino ewes

1962 ◽  
Vol 13 (4) ◽  
pp. 707 ◽  
Author(s):  
DR Lamond
Keyword(s):  
Ovarian Function ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Follicular Growth ◽  
Dual Effect ◽  
Final Injection ◽  
High Doses ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Ovarian Cycles

Following progesterone suppression of ovarian cycles, Merino ewes were given a combination of pregnant mare serum gonadotrophin (PMS) and human chorionic gonadotrophin (HCG) 1 or 2 days after the final injection of progesterone. The incidence of oestrus was recorded and the ewes were mated with entire rams. Laparotomy was carried out 2–3 days after oestrus to observe the number of ovulations and the follicular changes. The ewes were killed 5–6 weeks after treatment and conceptions noted. In non-pregnant ewes the incidence of cystic hyperplasia of the endometrium was recorded. The results may be summarized as follows:(a) Oestrus was inhibited in a high proportion of ewes receiving doses of 540 or 1620 i.u. of HCG. This was independent of the dose of PMS. (b) The number of large follicles was greatest at high doses of HCG. (c) In ewes receiving high doses of HCG, oestrus, if it occurred, was associated with large follicles. (d) Less than 10% of ewes conceived. (e) Endometrial cysts were present in a high proportion of ewes. It is suggested that HCG has a dual effect on ovarian function, depending on dose and the time of injection in relation to stage of follicular growth.

AUGMENTATION BY CHLORMADINONE OF THE UTERINE WEIGHT RESPONSE TO HUMAN CHORIONIC GONADOTROPHIN IN INTACT IMMATURE RATS

1965 ◽  
Vol 33 (3) ◽  
pp. 447-454
Author(s):  
M. J. K. HARPER
Keyword(s):  
Single Injection ◽  
Direct Action ◽  
Follicular Development ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Uterine Weight ◽  
Control Value ◽  
Orally Active ◽  
Stimulation Of

SUMMARY Administration of chlormadinone, an orally active progestational agent without significant oestrogenic activity, to intact immature female rats did not affect either ovarian or uterine weight significantly compared with controls. A single injection of human chorionic gonadotrophin (HCG) caused a 73 % increase in uterine weight in 24 hr. over the control value. This dose significantly increased ovarian weight and although it caused some stimulation of follicular development, ovulation during this time did not occur. When animals were treated with chlormadinone for 8 days, and received HCG on the 8th day, uterine weight was 170% greater than in the controls and 56% greater than with HCG alone. The uterine weight produced was similar to that found in animals treated with mestranol, a potent oestrogen, and HCG. In ovariectomized animals HCG did not affect uterine weight, while the small increase produced by chlormadinone was unaltered when HCG also was given. Mechanisms are discussed by which this augmentation of the uterine response to HCG might be produced. It seems most likely that chlormadinone administration causes storage of endogenous gonadotrophin in the pituitary, and that the exogenous gonadotrophin acts as the 'trigger' for the release of stored hormone, probably by a direct action on the hypothalamus.

Effects of thymulin on spontaneous puberty and gonadotrophin-induced ovulation in prepubertal normal and hypothymic mice

1999 ◽  
Vol 163 (2) ◽  
pp. 255-260 ◽  
Author(s):  
L Hinojosa ◽  
R Chavira ◽  
R Dominguez ◽  
P Rosas
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Vaginal Opening ◽  
Serum Progesterone ◽  
Thymic Hormone ◽  
Induced Ovulation ◽  
Gonadotrophin Secretion ◽  
Age Dependent ◽  
Neuroendocrine Mechanisms ◽  
Pregnant Mare Serum Gonadotrophin

The effects of thymulin administration beginning on days 19 or 24 of age on spontaneous puberty and gonadotrophin-induced ovulation were analysed in female normal and hypothymic mice. In normal and hypothymic mice, the daily administration of thymulin at 24 days of age resulted in a delay in the age of vaginal opening, with an increase in serum progesterone levels. Normal mice treated with 200 ng thymulin beginning on day 19 of age and injected with pregnant mare serum gonadotrophin (PMSG) 24 h later had an increase in ovulation rate, number of ova shed and weight of the ovaries. None of the hypothymic mice treated with thymulin on day 19 and PMSG on day 20 ovulated. PMSG treatment on day 25 induced ovulation in hypothymic mice. When these animals were injected previously with 200 ng thymulin, the number of ova shed by ovulating animals was lower than in PMSG-treated animals. Administration of thymulin and sequential injection of PMSG and human chorionic gonadotrophin 54 h later resulted in an increase in ovulatory response in comparison with those receiving only PMSG. The results suggest that thymulin plays a role in the regulation of spontaneous puberty through its effects on adrenal and ovarian endocrine functions. The increase in the ovarian PMSG response-treated animals, previously given thymulin, showed that this thymic hormone participates in the regulation of gonadotrophin secretion mechanisms and seems to be dose- and age-dependent. In hypothymic mice, neuroendocrine mechanisms regulating puberty are different from those of normal mice.

STIMULATION BY β2-ADRENERGIC RECEPTORS OF THE PRODUCTION OF CYCLIC AMP AND PROGESTERONE IN RAT OVARIAN TISSUE

1980 ◽  
Vol 87 (1) ◽  
pp. 123-129 ◽  
Author(s):  
ALBERT RATNER ◽  
G. K. WEISS ◽  
CAROLYN R. SANBORN
Keyword(s):  
Cyclic Amp ◽  
Adrenergic Receptors ◽  
Ovarian Tissue ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Adrenergic Stimulation ◽  
Immature Rats ◽  
Luteal Tissue ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulation Of

Ovarian tissue from immature rats treated with pregnant mare serum gonadotrophin (PMSG) or PMSG and human chorionic gonadotrophin was incubated in Medium 199. Stimulation of the formation of cyclic AMP in follicular and luteal tissue by terbutaline (10−5 mol/l), a selective β2-agonist, was blocked by butoxamine (10−5 mol/l), a selective β2-antagonist, whereas practolol (10−5 mol/l), a selective β1-antagonist, was ineffective. Propranolol (10−5 mol/l), a non-selective β-antagonist, butoxamine nor practolol affected the increase in cyclic AMP promoted by the addition of 1 μg LH. Stimulation of the production of progesterone in both follicular and luteal tissue by terbutaline was blocked by butoxamine, but not by practolol. These findings indicated that β-adrenergic stimulation of ovarian cyclic AMP and progesterone is mediated by β2-adrenergic receptors.

Control of the time of ovulation in sheep

1960 ◽  
Vol 11 (3) ◽  
pp. 389 ◽  
Author(s):  
AWH Braden ◽  
DR Lamond ◽  
HM Radford
Keyword(s):  
Optimal Control ◽  
Breeding Season ◽  
Artificial Insemination ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Treatment Schedule ◽  
Pregnant Mare Serum Gonadotrophin

Synchronization of ovulation in ewes during the breeding season was achieved by a 14 day course of progesterone injections followed by injections of pregnant mare serum gonadotrophin (PMS) and human chorionic gonadotrophin (HCG). Optimal control was attained when the PMS was given at the time of the last progesterone injection and the HCG 24 hr later. With this treatment most ovulations occurred between 20 and 28 hr after the HCG injection. However, none of the ewes exhibited oestrus at this time. Greater variability in the time of ovulation was found when the progesterone-PMS and PMS-HCG intervals were 0 hr and 48 hr, or 24 hr and 24 hr, and with these treatments about 80 per cent. of the ewes exhibited oestrus just before ovulation. On the 0-48 hr treatment schedule with artificial insemination 3-7½ hr after the HCG injection, 13 of 22 ewes had fertilized eggs or embryos when killed 3 or 35 days later.

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