ZONE ELECTROPHORESIS OF TOBACCO MOSAIC VIRUS

1959 ◽  
Vol 37 (1) ◽  
pp. 1025-1031 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ribonuclease Activity ◽  
Agar Gel ◽  
Zone Electrophoresis

An apparatus is described for the preparative zone electrophoresis of tobacco mosaic virus in agar gel. The isolated virus does not have any detectable ribonuclease activity.

ZONE ELECTROPHORESIS OF TOBACCO MOSAIC VIRUS

1959 ◽  
Vol 37 (8) ◽  
pp. 1025-1031 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ribonuclease Activity ◽  
Agar Gel ◽  
Zone Electrophoresis

An apparatus is described for the preparative zone electrophoresis of tobacco mosaic virus in agar gel. The isolated virus does not have any detectable ribonuclease activity.

THE INCORPORATION OF p-FLUOROPHENYLALANINE INTO TOBACCO MOSAIC VIRUS

1962 ◽  
Vol 40 (8) ◽  
pp. 999-1004 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Amino Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Free Fraction ◽  
Natural Amino Acid ◽  
Agar Gel ◽  
Tobacco Leaf ◽  
Zone Electrophoresis ◽  
Reduced Rate

Disks of diseased tobacco leaf tissue were treated with phenylalanine and with the analogue p-fluorophenylalanine. The expressed sap was separated into its components by agar gel zone electrophoresis. p-Fluorophenylalanine was shown to be incorporated into TMV at a reduced rate as compared with the natural amino acid phenylalanine. The analogue inhibited incorporation of phosphorus, a reversal probably due to the increase in phenylalanine observed in the soluble amino-acid-free fraction.

THE ELECTROPHORETIC ANALYSIS OF PLANT VIRUS PREPARATIONS

1959 ◽  
Vol 37 (1) ◽  
pp. 119-126 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Extracts ◽  
Plant Virus ◽  
Potato Virus X ◽  
Electrophoretic Analysis ◽  
Agar Gel ◽  
Zone Electrophoresis ◽  
Staining Methods ◽  
Protein Staining

Zone electrophoresis in agar gel has been used for separating tobacco mosaic virus and potato virus X from crude plant extracts and reaction mixtures. The viruses were located within the gel by autoradiographic, serological, and protein-staining methods.

THE INCORPORATION OF p-FLUOROPHENYLALANINE INTO TOBACCO MOSAIC VIRUS

1962 ◽  
Vol 40 (1) ◽  
pp. 999-1004
Author(s):  
P. M. Townsley
Keyword(s):  
Amino Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Free Fraction ◽  
Natural Amino Acid ◽  
Agar Gel ◽  
Tobacco Leaf ◽  
Zone Electrophoresis ◽  
Reduced Rate

Disks of diseased tobacco leaf tissue were treated with phenylalanine and with the analogue p-fluorophenylalanine. The expressed sap was separated into its components by agar gel zone electrophoresis. p-Fluorophenylalanine was shown to be incorporated into TMV at a reduced rate as compared with the natural amino acid phenylalanine. The analogue inhibited incorporation of phosphorus, a reversal probably due to the increase in phenylalanine observed in the soluble amino-acid-free fraction.

THE ELECTROPHORETIC ANALYSIS OF PLANT VIRUS PREPARATIONS

1959 ◽  
Vol 37 (1) ◽  
pp. 119-126 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Extracts ◽  
Plant Virus ◽  
Potato Virus X ◽  
Electrophoretic Analysis ◽  
Agar Gel ◽  
Zone Electrophoresis ◽  
Staining Methods ◽  
Protein Staining

Zone electrophoresis in agar gel has been used for separating tobacco mosaic virus and potato virus X from crude plant extracts and reaction mixtures. The viruses were located within the gel by autoradiographic, serological, and protein-staining methods.

On the ribonuclease activity associated with tobacco mosaic virus preparations

Virology ◽  
1963 ◽  
Vol 21 (2) ◽  
pp. 156-161 ◽  
Author(s):  
Paul R. Whitfeld ◽  
Shirley Williams
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ribonuclease Activity

Improved Methods for Infection of Plant Protoplasts with Viral Ribonucleic Acid

1978 ◽  
Vol 33 (7-8) ◽  
pp. 548-551 ◽  
Author(s):  
J. R. O. Dawson ◽  
P. E. Dickerson ◽  
Janet M. King ◽  
F. Sakai ◽  
A. R. H. Trim ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ribonucleic Acid ◽  
Ribonuclease Activity ◽  
Viral Rna ◽  
Plant Protoplasts ◽  
Tobacco Protoplasts ◽  
Improved Methods

Abstract Infection of tobacco protoplasts with RNA of tobacco mosaic virus and the bromoviruses has been studied. The quality of the RNA was very important. Ribonuclease activity was controlled by incorporating 5 mᴍ ZnCl2 in the inoculum. Up to 60% infection has been observed with bromovirus RNA but infection with TMV RNA was much less efficient. A procedure for inoculating in 40% polyethylene glycol was developed. High levels of infection were obtained with all the viruses and viral RNA examined.

Quantitative Measurements on the Ion Etching of TMV

1973 ◽  
Vol 31 ◽  
pp. 336-337
Author(s):  
Irwin Bendet ◽  
Nabil Rizk
Keyword(s):  
Electron Microscope ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ion Current ◽  
Beam Diameter ◽  
Ion Etching ◽  
Preliminary Results ◽  
Quantitative Measurements ◽  
Monitoring Devices

Preliminary results reported last year on the ion etching of tobacco mosaic virus indicated that the diameter of the virus decreased more rapidly at 10KV than at 5KV, perhaps reaching a constant value before disappearing completely.In order to follow the effects of ion etching on TMV more quantitatively we have designed and built a second apparatus (Fig. 1), which incorporates monitoring devices for measuring ion current and vacuum as well as accelerating voltage. In addition, the beam diameter has been increased to approximately 1 cm., so that ten electron microscope grids can be exposed to the beam simultaneously.

Sign in / Sign up

Export Citation Format

Share Document