THE INCORPORATION OF p-FLUOROPHENYLALANINE INTO TOBACCO MOSAIC VIRUS

1962 ◽  
Vol 40 (1) ◽  
pp. 999-1004
Author(s):  
P. M. Townsley
Keyword(s):  
Amino Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Free Fraction ◽  
Natural Amino Acid ◽  
Agar Gel ◽  
Tobacco Leaf ◽  
Zone Electrophoresis ◽  
Reduced Rate

Disks of diseased tobacco leaf tissue were treated with phenylalanine and with the analogue p-fluorophenylalanine. The expressed sap was separated into its components by agar gel zone electrophoresis. p-Fluorophenylalanine was shown to be incorporated into TMV at a reduced rate as compared with the natural amino acid phenylalanine. The analogue inhibited incorporation of phosphorus, a reversal probably due to the increase in phenylalanine observed in the soluble amino-acid-free fraction.

THE INCORPORATION OF p-FLUOROPHENYLALANINE INTO TOBACCO MOSAIC VIRUS

1962 ◽  
Vol 40 (8) ◽  
pp. 999-1004 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Amino Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Free Fraction ◽  
Natural Amino Acid ◽  
Agar Gel ◽  
Tobacco Leaf ◽  
Zone Electrophoresis ◽  
Reduced Rate

Disks of diseased tobacco leaf tissue were treated with phenylalanine and with the analogue p-fluorophenylalanine. The expressed sap was separated into its components by agar gel zone electrophoresis. p-Fluorophenylalanine was shown to be incorporated into TMV at a reduced rate as compared with the natural amino acid phenylalanine. The analogue inhibited incorporation of phosphorus, a reversal probably due to the increase in phenylalanine observed in the soluble amino-acid-free fraction.

THE ELECTROPHORETIC ANALYSIS OF PLANT VIRUS PREPARATIONS

1959 ◽  
Vol 37 (1) ◽  
pp. 119-126 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Extracts ◽  
Plant Virus ◽  
Potato Virus X ◽  
Electrophoretic Analysis ◽  
Agar Gel ◽  
Zone Electrophoresis ◽  
Staining Methods ◽  
Protein Staining

Zone electrophoresis in agar gel has been used for separating tobacco mosaic virus and potato virus X from crude plant extracts and reaction mixtures. The viruses were located within the gel by autoradiographic, serological, and protein-staining methods.

THE ELECTROPHORETIC ANALYSIS OF PLANT VIRUS PREPARATIONS

1959 ◽  
Vol 37 (1) ◽  
pp. 119-126 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Extracts ◽  
Plant Virus ◽  
Potato Virus X ◽  
Electrophoretic Analysis ◽  
Agar Gel ◽  
Zone Electrophoresis ◽  
Staining Methods ◽  
Protein Staining

Zone electrophoresis in agar gel has been used for separating tobacco mosaic virus and potato virus X from crude plant extracts and reaction mixtures. The viruses were located within the gel by autoradiographic, serological, and protein-staining methods.

scholarly journals RELATIONSHIPS BETWEEN TOBACCO MOSAIC VIRUS AND THE NON-VIRUS PROTEINS

1955 ◽  
Vol 38 (4) ◽  
pp. 475-492 ◽  
Author(s):  
Barry Commoner ◽  
Sidney D. Rodenberg
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Experimental Error ◽  
Leaf Tissue ◽  
Infected Leaf ◽  
Tobacco Leaf ◽  
Biochemical Processes ◽  
Cross Reactions ◽  
Immunochemical Techniques ◽  
Virus Proteins

1. The non-virus proteins, A4, B3, and B6, characteristically found in tobacco leaf infected with TMV exhibit specific immunochemical cross-reactions with serum prepared against the virus. The close immunochemical relations which occur among these proteins do not extend to any normal tobacco leaf proteins. 2. The rate of appearance of the non-virus proteins in newly infected cultured leaf tissue at various times after inoculation has been determined by immunochemical techniques and by direct isolation of the proteins. Both methods give comparable results. The non-virus proteins appear abruptly at about 220 hours after inoculation, when the TMV content is about one-third its final value. The amount of A4 rises rapidly and then levels off. The B6 content rises rapidly and continuously over the course of the experiment. B3 appears last, and increases in amount considerably more slowly than A4 and B6. 3. The isotope contents of TMV, B3, and B6 which appear in given intervals after inoculation in newly infected leaf cultured in nutrient containing N15H4 have been compared. The isotope levels of concurrent TMV, B3, and B6 are identical within the experimental error. The isotope conditions employed in this experiment lead to the conclusion that this coincidence of N15 levels means that the virus and non-virus proteins are probably synthesized at about the same time from the same non-protein source of nitrogen. 4. Possible interpretations of the available data on the non-virus proteins are discussed. It is likely that one or more of these proteins represents small protein units which occur in the TMV nucleoprotein.As they exist in the infected leaf, the non-virus proteins are probably no longer available to the biochemical processes which lead to TMV synthesis. They are probably not precursors of TMV protein in a temporal sense.

ZONE ELECTROPHORESIS OF TOBACCO MOSAIC VIRUS

1959 ◽  
Vol 37 (8) ◽  
pp. 1025-1031 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ribonuclease Activity ◽  
Agar Gel ◽  
Zone Electrophoresis

An apparatus is described for the preparative zone electrophoresis of tobacco mosaic virus in agar gel. The isolated virus does not have any detectable ribonuclease activity.

ZONE ELECTROPHORESIS OF TOBACCO MOSAIC VIRUS

1959 ◽  
Vol 37 (1) ◽  
pp. 1025-1031 ◽  
Author(s):  
P. M. Townsley
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ribonuclease Activity ◽  
Agar Gel ◽  
Zone Electrophoresis

An apparatus is described for the preparative zone electrophoresis of tobacco mosaic virus in agar gel. The isolated virus does not have any detectable ribonuclease activity.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

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