Invitro propagation of Fraser fir from embryonic explants

1991 ◽  
Vol 21 (3) ◽  
pp. 405-409 ◽  
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Butyric Acid ◽  
Adventitious Shoots ◽  
Adventitious Buds ◽  
Hypocotyl Explants ◽  
Fraser Fir ◽  
Growing Medium ◽  
Rooted Shoot

Cotyledons, hypocotyls, and cotyledon–hypocotyl explants were excised from seeds of Fraser fir (Abiesfraseri (Pursh) Poir.) subjected to H2O2 treatment for 3, 6, 9, or 12 days and cultured on media containing 0–30 mg/L N-(phenylmethyl)-1H-purine-6-amine. Adventitious buds developed on hypocotyls and hypocotyls of cotyledon–hypocotyl explants, but rarely on cotyledons. Excised hypocotyls produced more buds than hypocotyls of cotyledon–hypocotyl expiants (1.6 vs. 1.1). Hypocotyls excised from seeds treated with H2O2 for 6 or 9 days and placed on medium containing 10 mg/L N-(phenylmethyl)-1H-purine-6-amine yielded the greatest number of buds, with a mean of three buds per hypocotyl. Elongated adventitious shoots were placed on media containing 0–40 mg/L 1H-indole-3-butyric acid to induce rooting. Forty-eight percent of shoots treated with 40 mg/L 1H-indole-3-butyric acid rooted, and the greatest number of roots per rooted shoot (3.0) was also noted at this concentration. Plantlets were transferred to a growing medium, but acclimation was unsuccessful

scholarly journals In Vitro Propagation of Virginia Pine from Cotyledons

1991 ◽  
Vol 116 (2) ◽  
pp. 362-365 ◽  
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Butyric Acid ◽  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Adventitious Shoots ◽  
Growing Medium

Adventitious shoots developed on cotyledons of Virginia pine (Pinus virginiana Mill.) excised from seeds subjected to H2O2 treatment for 3, 6, or 9 days and cultured on media containing 0.5 to 10 mg BA/liter. Shoot regeneration was greatest (42 shoots per embryo) on cotyledons from seeds treated with H2O2 for 6 days and placed on medium containing BA at 10 mg·liter-1. Excised shoots elongated on medium lacking BA. Following elongation, shoots were placed on media containing IBA at 0 to 40 mg·liter-1 for 14 days followed by transfer to the same medium lacking auxin. Without IBA treatment, rooting was 3%, and increased to 50% for 5 to 40 mg·liter-1. Rooted shoots averaged 2.0 roots per shoot without auxin incorporation, 3.3 roots when treated with 5 mg IBA/liter, and the number of roots increased linearly with increased IBA concentration up to 40 mg·liter-1 (4.5 roots). Plantlets were transferred to growing medium and acclimated successfully to greenhouse conditions. Chemical names used: N- (phenylmethyl)-1 H- purine-6-amine (BA), 1 H- indole-3-butyric acid CBA).

scholarly journals Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata)

2018 ◽  
Vol 9 (3) ◽  
pp. 475-480
Author(s):  
Paulo Tarso Barbosa Sampaio ◽  
Lyana Silva Jardim ◽  
Ariel Dotto Blind ◽  
Flavio Mauro Souza Bruno
Keyword(s):  
Native Species ◽  
Callus Formation ◽  
Adventitious Shoots ◽  
Ms Medium ◽  
Adventitious Buds ◽  
Clonal Multiplication ◽  
Benefit Ratio ◽  
Dipteryx Odorata ◽  
Hypocotyl Segments

Somatic embryogenesis from callus induced in epicotyl and hypocotyl segments can be viable native species in order to better -benefit ratio costs, and rates of clonal multiplication. In this sense, two trials were established to induce callus and adventitious buds on hypocotyl and epicotyl segments of cumaru bean seedlings germinated in vitro in different concentrations and combinations of growth regulators. At first, we used the MS medium supplementwith ANA (0.0, 1.5 mg.L-1) and TDZ (0.0, 4.0 and 8.0 mg.L-1) distributed in factorial 2 x 3 x 2 (x auxin cytokinin x explant) with eight replications. In the second, it was used the WPM medium supplemented with BAP (2.0 mg L-1) and plus 2,4-D (2.0 and 4.0 mg L-1) in a factorial 2 x 2 (auxin x explant) with 15 repetitions each. They were evaluating callus formation and the average number of adventitious shoots during the period of 90 days. The results indicated that the highest average for callus formation was observed when the explants were subjected to concentrations of 8.0 mg L-1 TDZ combined with 1.5 mg L-1 ANA in MS medium. For the formation of buds, the WPM medium plus 2.0 mg L-1 2,4-D in the second experiment, induced higher number of shoots, being significant the use of auxin, and its interaction with the type of explant.

In vitro studies of adventitious shoot formation in Pinus contorta

1981 ◽  
Vol 59 (5) ◽  
pp. 870-874 ◽  
Author(s):  
Sara Von Arnold ◽  
Tage Eriksson
Keyword(s):  
Pinus Contorta ◽  
Stem Elongation ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Buds ◽  
Adventitious Shoot Formation ◽  
Bud Induction ◽  
Further Development

Isolated embryos of Pinus contorta Dougl. ex Loud, were induced to form adventitious buds on a cytokinin-supplemented medium. Further development of the buds required transfer to a cytokininless medium. Both bud induction and development were stimulated by a dilution of the basal culture medium and best growth was obtained if the buds were isolated from the original tissue when stem elongation had started. The growth of these isolated adventitious shoots was further stimulated by adding activated charcoal to the diluted medium. A small percentage of the shoots have been rooted. The capacity for bud formation varied among seeds collected from different regions of British Columbia. This method for induction of adventitious buds on embryos was also applicable to explants of young seedlings.

Production of adventitious shoots and plantlets from the hypocotyl explants of Sesbania rostrata (Bremek & obrem)

2002 ◽  
Vol 38 (5) ◽  
pp. 430-434 ◽  
Author(s):  
Ajay Kumar Jha ◽  
Surya Prakash ◽  
Neeru Jain ◽  
Kanan Nanda ◽  
S. C. Gupta
Keyword(s):  
Adventitious Shoots ◽  
Sesbania Rostrata ◽  
Hypocotyl Explants

scholarly journals IN VITRO PROPAGATION OF VIRGINIA PINE FROM COTYLEDONS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1101H-1102
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Adventitious Shoots ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Auxin Treatment ◽  
Growing Medium ◽  
Pinus Virginiana Mill

Adventitious shoots developed on cotyledons of Virginia pine (Pinus virginiana Mill.) excised from seeds germinated for 3, 6, or 9 days and cultured on media containing 0.5 to 10 mg/liter benzyladenine (BA). Shoot regeneration was greatest (46 shoots per embryo) on cotyledons from seeds germinated for 6 days and placed on medium containing 10 mg/liter BA. Shoots were excised and elongated on medium lacking BA. Following elongation, shoots were placed on media containing 0 to 40 mg/liter indolebutyric acid (IBA) for 14 days followed by transfer to the same medium lacking auxin. Without IBA treatment, percent rooting was 3% and increased to 50% for concentrations of 5 to 40 mg/liter. Rooted shoots averaged 2.0 roots per shoot without auxin treatment, 3.3 roots when treated with 5 mg/liter IBA and root number increased linearly with increased IBA concentration up to 40 mg/liter (4.5 roots). Plant lets were transferred to growing medium and acclimated successfully to greenhouse conditions.

scholarly journals In Vitro Rooting of Hypocotyl Cuttings of Fraser Fir

HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1650-1651 ◽  
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Butyric Acid ◽  
In Vitro Rooting ◽  
Rooted Cutting ◽  
Abies Fraseri ◽  
Fraser Fir ◽  
Hypocotyl Cuttings

Hypocotyl cuttings were prepared from Ii-week-old aseptically grown seedlings of Fraser fir [Abies fraseri (Pursh) Poir.] and cultured 18 days on media containing 0 to 40 mg IBA/liter followed by transfer to the same medium without auxin. Greatest rooting (66%) occurred after treatment with 20 mg IBA/liter, whereas the greatest number of roots per rooted cutting (7.4) was noted following treatment with 40 mg·liter-1. Chemical name used: 1H-indole-3-butyric acid (IBA).

Callus formation and organogenesis by explants of six Lycopersicon species

1983 ◽  
Vol 61 (4) ◽  
pp. 1072-1079 ◽  
Author(s):  
R. D. Locy
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Callus Formation ◽  
Adventitious Shoots ◽  
Poor Response ◽  
Shoot Formation ◽  
Callus Growth ◽  
Stem Explants ◽  
Hypocotyl Explants ◽  
Lycopersicon Species

Stem and hypocotyl explants of six Lycopersicon species, L. esculentum var. cerasiformae, L. cheesmanii, L. hirsutum, L. pimpinellifolium, L. peruvianum, and L. glandulosum, were examined for ability to form callus, adventitious shoots, and adventitious roots on media containing varying levels of indole acetic acid (IAA) and kinetin. Callus growth of L. peruvianum and L. glandulosum explants was better than that of the other species tested. Stem explants tended to form more callus than hypocotyl explants. Shoot formation by hypocotyl explants was superior to stem explants for all species and at nearly all hormone levels. Hypocotyl explants of L. peruvianum and L. glandulosum showed the highest levels of shoot formation, and L. cheesmanii and L. esculentum var. cerasiformae hypocotyl explants showed good shoot formation on media containing 3 mg/L kinetin and 0.3 or 1 mg/L indole acetic acid. No shoot formation was observed by L. hirsutum explants, and L. pimpinellifolium explants gave only a poor response. Shoot formation by stem explants was obtained only with L.. esculentum var. cerasiformae and L. glandulosum. When callus of the six species, subcultured for 63 days on a medium optimal for callus growth, was subcultured on shoot-forming media, only callus of L. peruvianum and L. glandulosum subsequently formed shoots. Callus of L. glandulosum has retained its morphogenic potential for 1 year in culture and can be used to initiate liquid suspension cultures.

scholarly journals (291) Adventitious Shoot Production and Transformation of Euonymus alata

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1081C-1081
Author(s):  
Alan G. Smith ◽  
Elizabeth S. Zimmermann
Keyword(s):  
Butyric Acid ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Kanamycin Resistance ◽  
Naphthaleneacetic Acid ◽  
Landscape Plant ◽  
Shoot Production ◽  
Four Levels ◽  
Number Of Shoots

Euonymus alata is an attractive landscape plant that has been reported to be an invasive species. Genetic modification through transformation is a method of reducing its invasiveness by producing sterile cultivars having limited or no seed production. A critical step in Agrobacterium-mediated gene transfer is the production of adventitious shoots. E. alata internodes and leaves from in vitro cultures were tested for adventitious shoot production on 16 plant growth regulator combinations: four levels of 6-benzylamino purine (BA) and three auxin treatments [0.5 or 0.25 mg·L-1 indole-3-butyric acid and 0.1 mg·L-1 naphthaleneacetic acid (NAA)], as well as no auxin. The optimal BA levels were found to be 0.5 or 1.0 mg·L-1 for maximizing the number of explants forming shoots and for producing the greatest number of shoots per explant. Culturing on NAA gave the greatest number of shoots per explant with both 0.5 and 1.0 mg·L-1 BA. Shoot production from internode segments was markedly superior to leaves. An initial dark treatment of 10 days did not influence shoot production. Using 1.0 mg BA with 0.1 mg·L-1 NAA, E. alata internodes were transformed with A. tumefaciens EHA105 carrying Kanamycin resistance and β-glucuronidase genes. Transformed shoots were selected on 30 mg·L-1 Kanamycin. Of the 36 shoots produced, 16 were confirmed to be transformed by β-glucuronidase histochemistry. Treatment with rooting powder containing indole-3-butyric acid did not aid rooting of shoots, but after 3 months in soil in high humidity, 21 of 24 E. alata shoots from tissue culture were rooted and acclimated.

scholarly journals In vitro organogenesis in watermelon cotyledons

2005 ◽  
Vol 40 (9) ◽  
pp. 861-865 ◽  
Author(s):  
Maria Graziela Zagatto Krug ◽  
Liliane Cristina Libório Stipp ◽  
Adriana Pinheiro Martinelli Rodriguez ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Apical Meristem ◽  
Callus Formation ◽  
Citrullus Lanatus ◽  
Histological Study ◽  
Adventitious Shoots ◽  
Proximal Region ◽  
Leaf Primordia ◽  
Adventitious Buds ◽  
In Vitro Organogenesis

The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-1) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants. Adventitious shoots were characterized by the development of shoot apical meristem and leaf primordia. The formation of protuberances, that do not develop into adventitious buds, was also observed.

scholarly journals Plant Regeneration from Anther-derived Embryos of Apple and Pear

HortScience ◽  
2002 ◽  
Vol 37 (6) ◽  
pp. 962-965 ◽  
Author(s):  
Masanori Kadota ◽  
Dong-Sheng Han ◽  
Yoshiji Niimi
Keyword(s):  
Plant Regeneration ◽  
Butyric Acid ◽  
Callus Formation ◽  
Adventitious Shoots ◽  
Pyrus Pyrifolia ◽  
Chromosome Counts ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Apple Cultivars

Anthers of six apple [Malus ×domestica (L.) Borkh.], three Japanese pear (Pyrus pyrifolia N.) and two European pear (Pyrus communis L.) scion cultivars were cultured. Callus formation occurred from anthers of all cultivars and androgenic embryogenesis was observed from all except P. pyrifolia `Kosui' and P. communis `La France'. Regeneration of adventitious shoots from anther-derived embryos was shown from all apple cultivars and P. pyrifolia `Shinko'. Many of these shoots did not grow or died on half-strength Murashige and Skoog medium (1962) with 4.4 μm BA and 0.5 μm IBA, whereas several shoots of apple `Starking Delicious' grew to plantlets. Chromosome counts of shoot apical cells of four clones derived from embryos of `Starking Delicious' showed that three clones were diploids and one clone comprised diploid and haploid shoots, suggesting that at least one clone originated from a microspore. Chemical names used: 3-indolyl-butyric acid (IBA); N6-benzyladenine (BA).

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