Production of adventitious shoots and plantlets from the hypocotyl explants of Sesbania rostrata (Bremek & obrem)

2002 ◽  
Vol 38 (5) ◽  
pp. 430-434 ◽  
Author(s):  
Ajay Kumar Jha ◽  
Surya Prakash ◽  
Neeru Jain ◽  
Kanan Nanda ◽  
S. C. Gupta
Keyword(s):  
Adventitious Shoots ◽  
Sesbania Rostrata ◽  
Hypocotyl Explants

Callus formation and organogenesis by explants of six Lycopersicon species

1983 ◽  
Vol 61 (4) ◽  
pp. 1072-1079 ◽  
Author(s):  
R. D. Locy
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Callus Formation ◽  
Adventitious Shoots ◽  
Poor Response ◽  
Shoot Formation ◽  
Callus Growth ◽  
Stem Explants ◽  
Hypocotyl Explants ◽  
Lycopersicon Species

Stem and hypocotyl explants of six Lycopersicon species, L. esculentum var. cerasiformae, L. cheesmanii, L. hirsutum, L. pimpinellifolium, L. peruvianum, and L. glandulosum, were examined for ability to form callus, adventitious shoots, and adventitious roots on media containing varying levels of indole acetic acid (IAA) and kinetin. Callus growth of L. peruvianum and L. glandulosum explants was better than that of the other species tested. Stem explants tended to form more callus than hypocotyl explants. Shoot formation by hypocotyl explants was superior to stem explants for all species and at nearly all hormone levels. Hypocotyl explants of L. peruvianum and L. glandulosum showed the highest levels of shoot formation, and L. cheesmanii and L. esculentum var. cerasiformae hypocotyl explants showed good shoot formation on media containing 3 mg/L kinetin and 0.3 or 1 mg/L indole acetic acid. No shoot formation was observed by L. hirsutum explants, and L. pimpinellifolium explants gave only a poor response. Shoot formation by stem explants was obtained only with L.. esculentum var. cerasiformae and L. glandulosum. When callus of the six species, subcultured for 63 days on a medium optimal for callus growth, was subcultured on shoot-forming media, only callus of L. peruvianum and L. glandulosum subsequently formed shoots. Callus of L. glandulosum has retained its morphogenic potential for 1 year in culture and can be used to initiate liquid suspension cultures.

scholarly journals A Rapid and Efficient Protocol for Adventitious Shoot Regeneration and Genetic Transformation of Manchurian Ash (Fraxinus mandshurica Rupr.) using Hypocotyl Explants

2020 ◽  
Author(s):  
Lin Liu ◽  
Yang Cao ◽  
Yaguang Zhan ◽  
Fenghui Qi
Keyword(s):  
Genetic Transformation ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Potassium Citrate ◽  
Regeneration System ◽  
Fraxinus Mandshurica ◽  
Hypocotyl Explants ◽  
Shoot Buds ◽  
Shoot Bud ◽  
Manchurian Ash

Abstract Background: Manchurian ash (Fraxinus mandshurica Rupr.) is an endangered hardwood tree species, providing both economic and medicinal benefits. However, observations such as browning of adventitious shoot buds and high rate of somatic embryo abnormality, were presented in protocols of F. mandshurica regeneration. Therefore, a rapid and high-efficiency regeneration system is demanded for mass propagation and genetic transformation of F. mandshurica.Results: We have developed an efficient regeneration system through adventitious shoot organogenesis in F. mandshurica using hypocotyl explants, with which the adventitious shoots are able to elongate and were obtained in an affordable time. Hypocotyls excised from embryos were pre-cultured in the dark on woody plant medium (WPM) supplemented with 6 g L -1 potassium citrate, and then inoculated on WPM medium supplemented with different concentrations of plant growth regulators (PGRs) to induce adventitious shoot bud formation. The induction medium supplemented with a single PGR of 1.0 mg L -1 thidiazuron (TDZ) was the best treatment, showing 86.67% shoot bud induction with a 15-day initial dark culture, followed by culture under a low light condition. The survival rate of regenerated shoot buds reached 70.97% on WPM medium supplemented with 0.025 mg L -1 TDZ and 1.0 mg L -1 gibberellic acid (GA3). Based on this regeneration system, By using the sonication plus vacuum-infiltration method,a protocol for Agrobacterium tumefaciens -mediated transformation of hypocotyls was established,the transformation rate was determined to be 3.57%. Conclusions: Key factors, such as the potassium citrate pretreatment, wound treatment on explants, variable light conditions, and significant PGR interactions, were revealed to affect the induction and elongation of adventitious shoots from F. mandshurica hypocotyls in this study. The adventitious shoots, tissue culture plantlets, and rooted plantlets were obtained at 40, 80-100, and 160 days, respectively. This regeneration system shortens the period of traditional regeneration methods, which require months to induce callus from leaves or stems, and additional several months for organ differentiation. In addition, the Agrobacterium-mediated transformation protocol established on the basis of this regeneration system provides a foundation for breeding, genetic improvement and genomic studies of F. mandshurica.

scholarly journals Micropropagation of Plantago camtschatica Link

2011 ◽  
Vol 77 (4) ◽  
pp. 269-273 ◽  
Author(s):  
Emilia Andrzejewska-Golec ◽  
Joanna Makowczyńska
Keyword(s):  
Medicinal Plant ◽  
In Vitro Culture ◽  
Far East ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Adventitious Shoot Regeneration ◽  
Hypocotyl Explants ◽  
The Far East

The Far East medicinal plant - <em>Plantago camtschatica</em> was propagated in vitro from tips of shoots (obtained in vitro) and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots, first leaves. To our knowledge there is no information in literature about in vitro culture of this plantain. MS basal medium, supplemented with 0.6 pM IAA in combination with various cytokinins (BA, KIN, ZEA), was used. After 6 weeks of culture, micropropagation rate (MR) - mean number of buds and shoots per explant - was calculated. Our study proved that <em>P. camtschatica</em> species was amenable to propagation in vitro from different kinds of explants. However, multiplication by adventitious shoot regeneration from hypocotyl explants was found to be the most suitable method for the propagation of this plant. Adventitious shoots could root without stimulation what allows to omit the stage of rooting. The plants obtained as a result of micropropagation were not phenotypically changed.

scholarly journals Adventitious Shoot Formation on Hypocotyl Explants of Antirrhinum majus L.

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 477B-477
Author(s):  
Kenneth R. Schroeder ◽  
Dennis P. Stimart
Keyword(s):  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Antirrhinum Majus ◽  
Fluorescent Light ◽  
Adventitious Shoot Formation ◽  
Hypocotyl Explants ◽  
Shoot Production ◽  
Dark Regime

One-centimeter hypocotyl explants from 2-week-old Antirrhinum majus L. (snapdragon) seedlings germinated and grown in vitro under 12-h cool-white fluorescent light and 12 h dark or 24 h dark were placed on Murashige and Skoog (MS) medium containing 0, 0.44, 2.22, 4.44, 8.88, or 44.4 μM N6-benzyladenine (BA). Cultures were maintained under the light/dark regime at 25°C. After 2 weeks, adventitious shoots were counted. A shoot was considered adventitious and counted if a stem and leaf developed. Shoots developed along the entire length of the hypocotyl sections. Mean shoot production per hypocotyl explant ranged from 2.4 to 6.1 shoots when seedlings were germinated and grown in 24 h darkness and 2.2 to 10.9 shoots when started in the light/dark regime. Highest shoot counts were attained /from hypocotyl explants when seedlings were germinated and grown under the light/dark regime for 2 weeks and transferred to 2.22, 4.44, or 8.88 μM BA. Shoot development appeared normal at the 2.22 and 4.44 μM level, while at 8.88 μM BA, development was slightly abnormal along with slightly more callus production.

Invitro propagation of Fraser fir from embryonic explants

1991 ◽  
Vol 21 (3) ◽  
pp. 405-409 ◽  
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Butyric Acid ◽  
Adventitious Shoots ◽  
Adventitious Buds ◽  
Hypocotyl Explants ◽  
Fraser Fir ◽  
Growing Medium ◽  
Rooted Shoot

Cotyledons, hypocotyls, and cotyledon–hypocotyl explants were excised from seeds of Fraser fir (Abiesfraseri (Pursh) Poir.) subjected to H2O2 treatment for 3, 6, 9, or 12 days and cultured on media containing 0–30 mg/L N-(phenylmethyl)-1H-purine-6-amine. Adventitious buds developed on hypocotyls and hypocotyls of cotyledon–hypocotyl explants, but rarely on cotyledons. Excised hypocotyls produced more buds than hypocotyls of cotyledon–hypocotyl expiants (1.6 vs. 1.1). Hypocotyls excised from seeds treated with H2O2 for 6 or 9 days and placed on medium containing 10 mg/L N-(phenylmethyl)-1H-purine-6-amine yielded the greatest number of buds, with a mean of three buds per hypocotyl. Elongated adventitious shoots were placed on media containing 0–40 mg/L 1H-indole-3-butyric acid to induce rooting. Forty-eight percent of shoots treated with 40 mg/L 1H-indole-3-butyric acid rooted, and the greatest number of roots per rooted shoot (3.0) was also noted at this concentration. Plantlets were transferred to a growing medium, but acclimation was unsuccessful

Utilization of Different Seedling Explants for in Vitro Propagation of Hibiscus syriacus

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 478e-479
Author(s):  
M.M. Jenderek ◽  
A.J. Olney
Keyword(s):  
Callus Formation ◽  
Adventitious Bud ◽  
Root Explants ◽  
Petiole Explants ◽  
Bud Formation ◽  
Hypocotyl Explants ◽  
Leaf Petiole ◽  
Adventitious Bud Formation ◽  
Hibiscus Syriacus

Hibiscus syriacus is a difficult species in micropropagation due to its endogenous contamination and recalcitrant shoot formation; therefore, studies on using explants other than shoot tip or axillary buds of growing shrubs were initiated. Three different seedling fragments (root, hypocotyl, and leaf petiole) from aseptically germinated seedlings of hibiscus (var. Aphrodite) were evaluated for adventitious bud formation, shoot and leaf development. The explants were cultured on McCown's woody plant basal salt medium supplemented with KNO3 (800 mg/L), adenine sulfate (80 mg/L) and MS vitamins containing BA or 2iP or TDZ at 0.5, 1.0, 2.2, 4.4 and 10 mM. Adventitious buds were present on all of the three different explants grown on medium containing TDZ; however, the most abundant bud formation, with many small leaves originating from callus was observed on hypocotyl explants cultured on medium with 1 mM of TDZ. Petiole explants were the most frequent to develop short shoots (≈15 mm) and one to nine leaves without callus formation, where 70% of hypocotyl and the root explants formed leaves originating from callus. Callus was induced on all explant types regardless of the level or type of cytokinin used. However, the number of shoots produced by any explant type was low, petioles cultured on 0.5 and 1mM of TDZ were the most suitable material for non-callus shoot development in H. syriacus. Hypocotyl explants proved to be an excellent source for adventitious bud formation but their ability to develop shoots needs to be investigated.

Chalcone reductase-homologous transcripts accumulate during development of stem-borne nodules on the tropical legume Sesbania rostrata

Planta ◽  
1999 ◽  
Vol 209 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Sofie Goormachtig ◽  
Sam Lievens ◽  
Sylvia Herman ◽  
Marc Van Montagu ◽  
Marcelle Holsters
Keyword(s):  
Sesbania Rostrata ◽  
Tropical Legume ◽  
Chalcone Reductase

High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale

2009 ◽  
Vol 53 (4) ◽  
pp. 769-773 ◽  
Author(s):  
X. G. Dai ◽  
X. P. Shi ◽  
Y. M. Ye ◽  
Q. Fu ◽  
M. Z. Bao
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Hypocotyl Explants ◽  
Ornamental Kale
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