Inheritance and linkage relationships of some isozymes of black spruce in New Brunswick

1985 ◽  
Vol 15 (5) ◽  
pp. 992-996 ◽  
Author(s):  
T. J. B. Boyle ◽  
E. K. Morgenstern
Keyword(s):  
Linkage Analysis ◽  
New Brunswick ◽  
Gel Electrophoresis ◽  
Black Spruce ◽  
Segregation Ratio ◽  
Pairwise Comparisons ◽  
Enzyme Systems ◽  
Independent Segregation ◽  
Haploid Megagametophyte ◽  
Linkage Relationships

Thirteen polymorphic loci from eleven enzyme systems in black spruce (Piceamariana (Mill.) B.S.P.) were studied by gel electrophoresis using embryos and haploid megagametophyte tissue. Two loci (Mdh-1 and Sdh) showed deviations from the expected 1:1 segregation ratio of megagametophytes from heterozygous maternal plants that were significant at the 1 % level and a third (Aat) at the 5% level. Of the 78 possible pairwise comparisons for the linkage analysis, 58 could be tested for jointly independent segregation. Linkage could be detected between three pairs of loci: Aat–Pgi, Gdh–Idh, and Fum–Pgm.

Inheritance and linkage of isozymes in white spruce (Picea glauca)

1983 ◽  
Vol 25 (5) ◽  
pp. 430-436 ◽  
Author(s):  
John N. King ◽  
Bruce P. Dancik
Keyword(s):  
Picea Glauca ◽  
White Spruce ◽  
Pairwise Comparisons ◽  
Mendelian Segregation ◽  
Polymorphic Loci ◽  
Linkage Analyses ◽  
Enzyme Systems ◽  
Variation Patterns ◽  
Joint Segregation ◽  
Haploid Megagametophyte

Variation patterns for 26 loci of white spruce (Picea glauca (Moench) Voss) from 16 enzyme systems were studied using haploid megagametophyte tissue. Inheritance models for 17 polymorphic loci were formulated and tested for deviations from Mendelian segregation. Linkage analyses were conducted for 12 of the polymorphic loci in pairwise comparisons. Significant joint segregation was found for three pairs of loci: Pgi-2 with Aat-1, Gdh with Idh, and one of three families tested with Pgi-2 and 6pg-2,3.

Inheritance and linkage of allozymes in seed tissue of whitebark pine

1986 ◽  
Vol 28 (4) ◽  
pp. 601-604 ◽  
Author(s):  
Glenn R. Furnier ◽  
Peggy Knowles ◽  
Mary A. Aleksiuk ◽  
Bruce P. Dancik
Keyword(s):  
Segregation Ratio ◽  
Pinus Albicaulis ◽  
Whitebark Pine ◽  
Enzyme Systems ◽  
Linkage Information ◽  
Haploid Megagametophyte ◽  
Significant Linkage

Allozyme variants were examined for 12 enzyme systems in haploid megagametophyte tissue of whitebark pine (Pinus albicaulis Engelm.). Inheritance and linkage information is reported for 11 loci (Aat-3, Aco, Adh, Fle-2, Idh, Mdh-2, Mdh-3, Mdh-4, Mdr, Pgi-2, and Pgm-2). Deviations from a 1:1 segregation ratio were observed at Fle-2, Pgi-2, and between two of the three alleles at Mdh-4. Significant linkage was observed for two pairs of loci (Aco–Fle-2 and Adh–Pgi-2), but estimates of recombination frequencies were relatively high.Key words: Pinus albicaulis, isozymes.

Structure et variabilité génétique de populations d'épinette noire (Piceamariana (Mill.) B.S.P.) dans la zone hémiarctique du Nouveau-Québec

1987 ◽  
Vol 17 (9) ◽  
pp. 1006-1012 ◽  
Author(s):  
Mireille Desponts ◽  
Jean-Pierre Simon
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Gel Electrophoresis ◽  
Black Spruce ◽  
Geographic Isolation ◽  
Marginal Populations ◽  
Isolated Populations ◽  
Subarctic Region ◽  
Enzyme Systems

The genetic structure of five populations of black spruce located at l'Eau Claire Lake, in the subarctic region of Nouveau-Québec, were analysed by acrylamide gel electrophoresis of seven enzyme systems. The analysis of 25 loci disclosed an average polymorphism of 0.80 and a level of heterozygosity of 0.351. Results indicate that there is no loss of genetic variability in these marginal populations that could be attributed to genetic drift. Data based on Wright's statistics indicate an excess of heterozygotes for most loci. Populations share the same alleles at most loci and are only slightly differentiated from each other (Fst = 0.06). Results suggest that gene flow between these isolated populations remains sufficiently high to override the effect of geographic isolation.

scholarly journals Inheritance and linkage relationships of allozyme variants of ilex paraguariensis St. Hil

2009 ◽  
Vol 52 (6) ◽  
pp. 1443-1451
Author(s):  
Simone Neumann Wendt ◽  
Valderês Aparecida de Sousa ◽  
Alexandre Magno Sebbenn ◽  
José Alfredo Sturion ◽  
Felipe Luíz ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Segregation Distortion ◽  
Gel Electrophoresis ◽  
Ilex Paraguariensis ◽  
Starch Gel Electrophoresis ◽  
Polymorphic Loci ◽  
Gene Segregation ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Linkage Relationships

Eighteen enzyme systems were examined in Ilex paraguariensis St. Hil. using starch gel electrophoresis. Seven out of 12 active isozyme systems revealed one or more polymorphic loci (PGI, GOT, MR, G-6PDH, MDH, NDH, and 6-PGDH). However, the segregation and linkage analyses were performed only for PGI, GOT, G-6PDH and 6-PGDH systems. Gene segregation at these loci was regular, except for a few trees that showed segregation distortion. Weak linkage disequilibrium between loci was detected, but it was not enough to influence the multilocus estimate.

Genetics of isozyme variants and linkage relationships among allozyme loci in 35 eastern white pine clones

1981 ◽  
Vol 11 (3) ◽  
pp. 573-579 ◽  
Author(s):  
R. T. Eckert ◽  
R. J. Joly ◽  
D. B. Neale
Keyword(s):  
Gel Electrophoresis ◽  
Direct Evidence ◽  
Evidence Based ◽  
Starch Gel Electrophoresis ◽  
White Pine ◽  
Chi Square ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Allozyme Loci ◽  
Linkage Relationships

Megagametophyte tissue from seeds of 35 clones of Pinusstrobus L. were subjected to horizontal starch gel electrophoresis. The resulting gels were tested for activity of 10 enzyme systems. Isozymes observed were under control of at least 17 loci. Direct evidence, based on segregation of allozymes in megagametophytes of heterozygous clones, is presented for nine loci in five systems (LAP, GPI, GOT, PGM, and AP). Three polymorphic loci were detected for LAP. No genetic variation was detected for IDH, ADH, GLY-3-PD, GDH, or 6 PDG.Five pairs of linked loci were detected by chi-square analyses of joint segregation. Twenty-five of 36 possible two-locus combinations among 18 clones were evaluated. Recombination estimates suggest relatively strong linkage between LAP1-LAP2 and GPI2-GOT1; weaker linkage between LAP2-GPI2 (based on one clone only); and weak linkage between LAP2-AP1 and GOT3-AP1.

Evidence for a new form of dominant negative imprinting at the ovine callipyge locus

1996 ◽  
Vol 1996 ◽  
pp. 51-51
Author(s):  
N.E. Cockett ◽  
S.P. Jackson ◽  
T.L. Shay ◽  
F. Farnir ◽  
S. Berghmans ◽  
...  
Keyword(s):  
Strong Evidence ◽  
Distal Part ◽  
Segregation Ratio ◽  
Dominant Negative ◽  
Chromosome 18 ◽  
Dominant Mutation ◽  
Independent Segregation ◽  
Parental Imprinting ◽  
New Form ◽  
Unusual Phenotype

In 1983, the occurrence of a ram exhibiting an exceptional muscular development was reported in a flock of Dorset sheep. This unusual phenotype was transmitted to some of the offspring, and subsequent matings involving hypermuscled male descendents of the founder ram and normal ewes clearly demonstrated a 50%-50%, sex-independent segregation ratio of the trait. It was therefore postulated that a dominant mutation (CLPG versus the normal clpg allele) at the autosomal “callipyge“ locus was responsible for this hypertrophy. Using the same family material, the callipyge locus was unambiguously mapped to the distal part of ovine chromosome 18 (Cockett et al., 1994).In this paper, we present very strong evidence that the callipyge locus is subjected to a previously undescribed form of parental imprinting. It is indeed demonstrated that animals only express the callipyge phenotype if they have inherited the CLPG mutation from their sire, not if it is transmitted by the dam.

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

1988 ◽  
Vol 66 (3) ◽  
pp. 588-594 ◽  
Author(s):  
Hong Zhu ◽  
Kenneth O. Higginbotham ◽  
Bruce P. Dancik ◽  
Stan Navratil
Keyword(s):  
Genetic Variability ◽  
Host Selection ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Ectomycorrhizal Fungus ◽  
Habitat Isolation ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Forest Regions ◽  
Starch Gel

Mycelial extracts of 43 isolates of Suillus tomentosus (Kauffm.) Singer, Snell & Dick collected from four boreal forest regions in Alberta were subjected to starch gel electrophoresis. A total of 21 bands was resolved from eight different enzyme systems presumably representing 13 loci. Six loci were polymorphic among these isolates. Cluster and principal components analyses demonstrated that intraspecific genetic variability of this fungus existed among and within forest regions. Polymorphic loci of acid phosphatase and alkaline phosphatase exhibited the greatest genetic similarity among the isolates within forest regions. Habitat isolation and host selection could be the major sources of genetic variation among forest regions.

Isozyme Variation in Broom Snakeweed (Gutierrezia sarothrae)

Weed Science ◽  
1995 ◽  
Vol 43 (1) ◽  
pp. 156-162 ◽  
Author(s):  
Yanglin Hou ◽  
Tracy M. Sterling
Keyword(s):  
Cluster Analysis ◽  
New Mexico ◽  
Genetic Variability ◽  
Gel Electrophoresis ◽  
Isozyme Analysis ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Morphological Differences ◽  
Gutierrezia Sarothrae

Broom snakeweed, a perennial rangeland shrub, is highly variable morphologically and can grow under a broad range of environmental conditions. In this study, isozyme analysis using starch gel electrophoresis was used to quantify genetic variability within and among New Mexico populations of broom snakeweed. Eight separate populations of broom snakeweed and one population of threadleaf snakeweed as a comparison were investigated. of the 10 enzyme systems examined, 16 loci were identified in eight populations and two species. Eleven loci were monomorphic in eight populations and two species and five loci were polymorphic in at least one population or species. Genetic variability was large in broom and threadleaf snakeweed populations as determined by isozyme analysis. Genetic variability among broom snakeweed populations was greater than that within populations for the five polymorphic loci. Cluster analysis of genetic distance and identity for the eight populations and two species characterized two major groups. Within broom snakeweed, cluster analysis characterized five groups. The two species shared most common alleles. The genetic variation identified in this research may account for the morphological differences and broad geographical distribution of broom snakeweed.

Individual height–diameter models for young black spruce (Picea mariana) and jack pine (Pinus banksiana) plantations in New Brunswick, Canada

2009 ◽  
Vol 85 (1) ◽  
pp. 43-56 ◽  
Author(s):  
Xiangdong Lei ◽  
Changhui Peng ◽  
Haiyan Wang ◽  
Xiaolu Zhou
Keyword(s):  
New Brunswick ◽  
Picea Mariana ◽  
Pinus Banksiana ◽  
Black Spruce ◽  
Jack Pine ◽  
Tree Level ◽  
Forest Stands ◽  
Tolerance Intervals ◽  
Group I ◽  
Group Ii

Historically, height–diameter models have mainly been developed for mature trees; consequently, few height–diameter models have been calibrated for young forest stands. In order to develop equations predicting the height of trees with small diameters, 46 individual height–diameter models were fitted and tested in young black spruce (Picea mariana) and jack pine (Pinus banksiana) plantations between the ages of 4 to 8 years, measured from 182 plots in New Brunswick, Canada. The models were divided into 2 groups: a diameter group and a second group applying both diameter and additional stand- or tree-level variables (composite models). There was little difference in predicting tree height among the former models (Group I) while the latter models (Group II) generally provided better prediction. Based on goodness of fit (R2and MSE), prediction ability (the bias and its associated prediction and tolerance intervals in absolute and relative terms), and ease of application, 2 Group II models were recommended for predicting individual tree heights within young black spruce and jack pine forest stands. Mean stand height was required for application of these models. The resultant tolerance intervals indicated that most errors (95%) associated with height predictions would be within the following limits (a 95% confidence level): [-0.54 m, 0.54 m] or [-14.7%, 15.9%] for black spruce and [-0.77 m, 0.77 m] or [-17.1%, 18.6%] for jack pine. The recommended models are statistically reliable for growth and yield applications, regeneration assessment and management planning. Key words: composite model, linear model, model calibration, model validation, prediction interval, tolerance interval

Isolation of fungal endophytes from black spruce (Picea mariana) dormant buds and needles from New Brunswick, Canada

1992 ◽  
Vol 70 (9) ◽  
pp. 1754-1757 ◽  
Author(s):  
J. A. Johnson ◽  
N. J. Whitney
Keyword(s):  
New Brunswick ◽  
Key Words ◽  
Endophytic Fungi ◽  
Picea Mariana ◽  
Black Spruce ◽  
Fungal Endophytes ◽  
The Other ◽  
Age Classes ◽  
Needle Age

Endophytic fungi were isolated from black spruce (Picea mariana (Mill.) BSP) dormant buds and needles of four age-classes. Only one isolate was obtained from 400 buds. A total of 914 isolates were obtained from the needles. With increasing needle age the rate of colonization increased between current-year and 3-year-old needles from 4 to 90%, respectively. The needle segment attached to the twig was colonized more often (p < 0.05) than other segments overall and for 6 of the 11 taxa isolated. The first endophyte from current-year needles was isolated on July 14, 1988, but endophytes were obtained from needles of the other age-classes (1, 2, and 3 years) on all sampling dates between June 10 and September 16, 1988. Key words: endophyte, Picea mariana, dormand buds, needles.

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