The structure of the antigenic lipopolysaccharide O-chain produced by Escherichiahermannii ATCC 33650 and 33652

1990 ◽  
Vol 68 (8) ◽  
pp. 1456-1466 ◽  
Author(s):  
Linda M. Beynon ◽  
David R. Bundle ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Molecular Modelling ◽  
Partial Hydrolysis ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Structure Formula ◽  
Structural Methods ◽  
Nuclear Magnetic

High resolution two-dimensional 1H and 13C nuclear magnetic resonance at 500 MHz was used in combination with molecular modelling to solve the structures of the antigenic O-polysaccharides produced by Escherichiahermannii strains ATCC 33650 and 33652. Classical structural methods such as methylation analysis, selective and partial hydrolysis, and periodate oxidations confirmed that the O-polysaccharides had a branched tetrasaccharide repeating unit with the structure:[Formula: see text]Keywords: Escherichiahermannii, lipopolysaccharide, magnetic resonance, polysaccharide.

Structure of the lipopolysaccharide O-antigen of Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590)

1989 ◽  
Vol 67 (4) ◽  
pp. 703-707 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Hydrolytic Degradation ◽  
Resonance Spectroscopy ◽  
Two Dimensional ◽  
O Antigen ◽  
Structure Formula ◽  
Nuclear Magnetic

One- and two-dimensional high resolution 1H and 13C nuclear magnetic resonance spectroscopy at 500 MHz and 125 MHz together with methylation and partial hydrolytic degradation methods were used to determine the structure of the antigenic O-polysaccharide produced by Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590). The O-polysaccharide was shown to be a high molecular weight unbranched linear polymer of repeating pentasaccharide units having the structure:[Formula: see text].Keywords: Actinobacilluspleuropneumoniae, lipopolysaccharide, O-antigen, nuclear magnetic resonance.

Structure of the lipopolysaccharide antigenic O-chain produced by Salmonella livingstone (O:6,7)

1989 ◽  
Vol 67 (6) ◽  
pp. 278-280 ◽  
Author(s):  
Jose Luis Di Fabio ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Key Words ◽  
Methylation Analysis ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The lipopolysaccharide produced by Salmonella livingstone (O:6,7) was composed of an antigenic O-polysaccharide which was shown by composition, methylation analysis and high resolution nuclear magnetic resonance studies to be a high molecular weight polymer containing D-glucose, 2-acetamido-2-deoxy-D-glucose and D-mannose residues (1:1:4) composed in a repeating hexasaccharide unit having the structure:[Formula: see text]Key words: Salmonella livingstone, lipopolysaccharide, O-polysaccharide.

Characterisation of the Salmonella O:8 antigen in the O-chain of the lipopolysaccharide produced by Salmonella Virginia O:8

1991 ◽  
Vol 69 (12) ◽  
pp. 852-858 ◽  
Author(s):  
Leann L. MacLean ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Acid Hydrolysis ◽  
Two Dimensional ◽  
Partial Acid Hydrolysis ◽  
Salmonella Endotoxin ◽  
Antigenic Factor ◽  
Structure Formula ◽  
Nuclear Magnetic

The antigenic O-polysaccharide of the lipopolysaccharide produced by Salmonella Virginia (O:8), analyzed by methylation, partial acid hydrolysis, and one- and two-dimensional nuclear magnetic resonance methods, was found to be a polymer of a repeating pentasaccharide unit composed of D-mannose, D-galactose, L-rhamnose, D-abequose, and O-acetyl (2:1:1:1:1.3) and having the following structure:[Formula: see text]The disaccharide structure α-D-Abep-(1 → 3)-L-Rhap was identified as the Salmonella O:8 antigenic factor epitope, since the removal of α-D-Abep residues from the O-polysaccharide left a residual tetrasaccharide repeating unit backbone that did not show reaction with Salmonella type O:8 factor antiserum.Key words: Salmonella, endotoxin, lipopolysaccharide, polysaccharide, nuclear magnetic resonance.

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