Structure of the lipopolysaccharide O-antigen of Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590)

1989 ◽  
Vol 67 (4) ◽  
pp. 703-707 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Hydrolytic Degradation ◽  
Resonance Spectroscopy ◽  
Two Dimensional ◽  
O Antigen ◽  
Structure Formula ◽  
Nuclear Magnetic

One- and two-dimensional high resolution 1H and 13C nuclear magnetic resonance spectroscopy at 500 MHz and 125 MHz together with methylation and partial hydrolytic degradation methods were used to determine the structure of the antigenic O-polysaccharide produced by Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590). The O-polysaccharide was shown to be a high molecular weight unbranched linear polymer of repeating pentasaccharide units having the structure:[Formula: see text].Keywords: Actinobacilluspleuropneumoniae, lipopolysaccharide, O-antigen, nuclear magnetic resonance.

The structure of the antigenic lipopolysaccharide O-chain produced by Escherichiahermannii ATCC 33650 and 33652

1990 ◽  
Vol 68 (8) ◽  
pp. 1456-1466 ◽  
Author(s):  
Linda M. Beynon ◽  
David R. Bundle ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Molecular Modelling ◽  
Partial Hydrolysis ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Structure Formula ◽  
Structural Methods ◽  
Nuclear Magnetic

High resolution two-dimensional 1H and 13C nuclear magnetic resonance at 500 MHz was used in combination with molecular modelling to solve the structures of the antigenic O-polysaccharides produced by Escherichiahermannii strains ATCC 33650 and 33652. Classical structural methods such as methylation analysis, selective and partial hydrolysis, and periodate oxidations confirmed that the O-polysaccharides had a branched tetrasaccharide repeating unit with the structure:[Formula: see text]Keywords: Escherichiahermannii, lipopolysaccharide, magnetic resonance, polysaccharide.

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