THE ULTRAVIOLET SPECTRA OF RIBONUCLEIC ACIDS IN SOLID STATE AND IN SOLUTION

1965 ◽  
Vol 43 (12) ◽  
pp. 3151-3159 ◽  
Author(s):  
Michael Falk
Keyword(s):  
Aqueous Solution ◽  
Solid State ◽  
Secondary Structure ◽  
Relative Humidity ◽  
Ribonucleic Acid ◽  
Room Temperature ◽  
Deoxyribonucleic Acid ◽  
Ribonucleic Acids ◽  
Solid Films ◽  
Spectral Changes

The ultraviolet absorption spectra of nine samples of soluble, ribosomal, and viral ribonucleic acid (RNA) were studied over the range 3 500 to 1 830 Å. The spectra and the spectral changes which occur upon denaturation were the same for all types of RNA examined, which suggests that their secondary structure is comparable. Dehydration of solid films of RNA at room temperature caused spectral changes resembling those which occur when RNA is heated in aqueous solution. This indicates that the dehydration of RNA, like the dehydration of deoxyribonucleic acid (DNA), causes some loss of the regular stacking and pairing of the nucleotide bases in the helical regions. In contrast to DNA, the rehydration of RNA was not always complete at 98% relative humidity. The decrease of absorbance in the 2 900 Å region upon denaturation, previously reported by Rich and Kasha, was not confirmed with any of the RNA samples studied.

Molecular Dynamics Study on an Adipokinetic Hormone Peptide in Aqueous Solution

2000 ◽  
Vol 55 (1-2) ◽  
pp. 125-128 ◽  
Author(s):  
Igor Z. Zubrzycki
Keyword(s):  
Molecular Dynamics ◽  
Aqueous Solution ◽  
Molecular Dynamics Simulation ◽  
Secondary Structure ◽  
Room Temperature ◽  
Dynamics Simulation ◽  
Red Pigment ◽  
Adipokinetic Hormone ◽  
Extended Conformation ◽  
Flying Insects

Lom-AKH-I is a member of the adipokinetic hormone/ red pigment concentrating hormone (AKH / RPCH) family of peptides found in flying insects. A molecular dynamics simulation at room temperature (293 K) in water has been performed to survey the folding path of the Lom-AKH-I peptide in water and to establish the secondary structure of Lom-AKH-I. The obtained results indicate the presence of an undefined extended conformation.

High performance room temperature all-solid-state Na-Se S battery with Na3SbS4–coated cathode via aqueous solution

2020 ◽  
Vol 48 ◽  
pp. 250-258 ◽  
Author(s):  
Ziqi Zhang ◽  
Haonan Cao ◽  
Meng Yang ◽  
Xinlin Yan ◽  
Chuang Yu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Solid State ◽  
High Performance ◽  
Room Temperature

A GRAVIMETRIC STUDY OF HYDRATION OF POLYNUCLEOTIDES

1966 ◽  
Vol 44 (10) ◽  
pp. 1107-1111 ◽  
Author(s):  
Michael Falk
Keyword(s):  
Relative Humidity ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Smooth Functions ◽  
A Value ◽  
General Similarity ◽  
Phosphate Groups

The hydration of soluble ribonucleic acid, of two forms of polyriboadenylic acid, and of nucleohistone was determined gravimetrically as a function of relative humidity (r.h.). The curves are smooth functions of r.h., similar to those obtained earlier for deoxyribonucleic acid. The general similarity of the hydration curves of the polynucleotides so far investigated is in agreement with the suggestion that the ionic phosphate groups are the primary hydration sites in polynucleotides. The hydration data fit the Brunauer–Emmett–Teller equation up to about 75% r.h. and the Harkins–Jura equation at higher values. Both equations yield a value of about 2.0 molecules of H2O per nucleotide for the "monolayer content" for all the polynucleotides investigated.

Radiation Chemistry of Carbohydrates, X* γ-Radiolysis of Crystalline D-Glucose and D-Fructose

1977 ◽  
Vol 32 (2) ◽  
pp. 213-224 ◽  
Author(s):  
M. Dizdaroglu ◽  
D. Henneberg ◽  
K. Neuwald ◽  
G. Schomburg ◽  
C. Von Sonntag
Keyword(s):  
Aqueous Solution ◽  
Solid State ◽  
Dose Rate ◽  
Gluconic Acid ◽  
Room Temperature ◽  
Radiation Chemistry ◽  
Chain Reaction ◽  
A Chain ◽  
Reaction Schemes

α-D-Glucose and β-D-fructose were γ-irratiated in the solid (polycrystalline) state at room temperature at doses of 3.5 · 1020-4.2 · 1021 eV g-1 (dose rate 1.16 · 1018 eV g-1 min-1). Carbohydrate products containing ≤ 6 carbon atoms were identified and their G-values (in parentheses) measured.Glucose: Dihydroxyacetone (1) (0.05), 3-deoxy-tetrose (2) (0.015), 1,4-dideoxy-2-pentulose (3) (0.05), 2,4-dideoxy-pentose (4) (0.085), 2,4-dideoxy-pentonic acid (5), 2,3-dideoxypentos-4-ulose (6) (together 0.035), threose (7), erythrulose (8), erythrose (9), erythronic acid (10) (together 0.04), 1-deoxy-2-pentulose (11) (0.005), 2-deoxy-ribose (12) (0.25), 3-deoxy-pentosulose (18) (0.02), 3,5-dideoxy-hexonic acid (14) (0.02), 2,3-dideoxy-hexonic acid (15) (0.01), arabinose (16) (0.25), ribose (17), ribonic acid (18) (together 0.02), 2-deoxy-2-C-hydroxymethyl-pentonic acid (19) (0.06), 5-deoxy-gluconic acid (20), 2deoxy-5-keto-glucose (21), 2-deoxy-gluconic acid (22), 2-deoxy-3-keto-glucose (28), 3-deoxy-glucosone (24), 3-deoxy-gluconic acid (25), 3-deoxy-4-keto-glucose (26), 3-deoxymannonic acid (27) (together 0.4). Identified but nor measured quantitatively were glucosone (28), 3-keto-glucose (29), 4-keto-glucose (30), 5-keto-glucose (31) and gluconic acid (82). G(H2) = 5.75; G(CO2) = 0.7.Fructose: 7-9 (together 0.65), 3-deoxy-pentonic acids (37), 3-deoxy-pentosulose (88) (together 0.3), arabonic acid (89) (0.1), 18 (0.05), 6-deoxy-2,5-hexodiulose (40) (40). Identified but not measured quantitatively were glyceraldehyde (34), butanone-(3)-diol-(1,2) (35) and 2- and 3-deoxy-hexodiuloses. G(H2) = 4.75, G(CO2) = 0.05.Reaction schemes are proposed to account for the formation of the products. The scission of the hemiacetal bond and of the C-Η and C-C bonds next to it appears to be typical for solid state irradiations. The formation of deoxy-compounds is observed both in the solid state and in aqueous solution. The formation of dideoxy-compounds is only prominent in the solid state. In polycrystalline fructose a chain reaction is induced leading to 6-deoxy-2,5-hexodiulose (40).

Humidity Sensitive Properties of Graphene Oxide Investigated by Quartz Crystal Microbalance

2014 ◽  
Vol 1051 ◽  
pp. 85-89 ◽  
Author(s):  
Hui Yao Yu ◽  
Ying Long Yao ◽  
Xiao Hua Wang
Keyword(s):  
Thin Film ◽  
Aqueous Solution ◽  
Graphene Oxide ◽  
Relative Humidity ◽  
Quartz Crystal Microbalance ◽  
Room Temperature ◽  
Quartz Crystal ◽  
Oxide Thin Film ◽  
Sensing Material ◽  
Maximum Humidity

Graphene oxide has been studied as sensing material for the humidity detection in this paper. At room temperature, graphene oxide was dissolved in water to prepare graphene oxide aqueous solution. This aqueous solution was distributed on the electrode surface of quartz crystal microbalance to form a thin film for humidity detection. The results of the experiment showed that the quartz crystal microbalance sensors with graphene oxide film have good response to the change of humidity. The maximum humidity sensitivity, during the humidity ranging from 10% to 90%RH (relative humidity), has achieved ~54Hz/%RH (relative humidity). The quartz crystal microbalance sensors with graphene oxide thin film have good stability and reproducibility properties. All results implied that the graphene oxide was a potential humidity sensing material for practical use.

scholarly journals Säurehydrolyse von Ribonucleinsäure und Desoxyribonucleinsäure

1961 ◽  
Vol 16 (10) ◽  
pp. 673-678 ◽  
Author(s):  
W. Pollmann ◽  
G. Schramm
Keyword(s):  
Enzymatic Hydrolysis ◽  
Nucleic Acids ◽  
Potentiometric Titration ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Potentiometric Method ◽  
Ribonucleic Acids ◽  
Rate Of Hydrolysis ◽  
Phosphate Groups

A method for the potentiometric titration of secondary phosphate groups in nucleic acids is described. Ribonucleic acids of yeast and of microsomes contain 5—6% secondary phosphate groups which cannot be removed by dialysis. The potentiometric method was applied to study several enzymatic hydrolyses and the non-enzymatic hydrolysis between pH 2.4 —1.8. The rate of hydrolysis for the purines and for the phosphate groups is approximately proportional to the H®-concentration. The constants of hydrolysis for ribonucleic acid and for deoxyribonucleic acid were determined. In DNA the depurinisation is 650 times faster than in RNA.

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