A GRAVIMETRIC STUDY OF HYDRATION OF POLYNUCLEOTIDES

1966 ◽  
Vol 44 (10) ◽  
pp. 1107-1111 ◽  
Author(s):  
Michael Falk
Keyword(s):  
Relative Humidity ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Smooth Functions ◽  
A Value ◽  
General Similarity ◽  
Phosphate Groups

The hydration of soluble ribonucleic acid, of two forms of polyriboadenylic acid, and of nucleohistone was determined gravimetrically as a function of relative humidity (r.h.). The curves are smooth functions of r.h., similar to those obtained earlier for deoxyribonucleic acid. The general similarity of the hydration curves of the polynucleotides so far investigated is in agreement with the suggestion that the ionic phosphate groups are the primary hydration sites in polynucleotides. The hydration data fit the Brunauer–Emmett–Teller equation up to about 75% r.h. and the Harkins–Jura equation at higher values. Both equations yield a value of about 2.0 molecules of H2O per nucleotide for the "monolayer content" for all the polynucleotides investigated.

THERMAL ANALYSIS OF POLYANION METACHROMASY: EFFECT OF TEMPERATURE ON METACHROMATIC SOLUTIONS OF NUCLEIC ACIDS AND ACID MUCOPOLYSACCHARIDES

1969 ◽  
Vol 17 (10) ◽  
pp. 651-657 ◽  
Author(s):  
JOHN W. KELLY ◽  
LOUIS CHANG ◽  
FRED ROTHSTEIN
Keyword(s):  
Nucleic Acids ◽  
Toluidine Blue ◽  
Ribonucleic Acid ◽  
Equilibrium Constants ◽  
Linear Functions ◽  
Effect Of Temperature ◽  
A Value ◽  
Acid Mucopolysaccharides ◽  
Common Equation ◽  
Phosphate Groups

Spectra of toluidine blue with acid mucopolysaccharides and nucleic acids were recorded over the temperature range 10-70°C. Metachromatic ratios were inverse, linear functions of temperature. Intercepts and slopes of the thermal plots distinguished acid mucopolysaccharides from nucleic acids. Acid mucopolysaccharide reactions were thermally reversible. Nucleic acid reactions were not strictly reversible; such behavior was not attributable to thermal denaturation. An explanation for the unusual γ-peak of ribonucleic acid-toluidine blue metachromasy is offered in terms of terminal phosphate groups of ribonucleic acid. Solution data in which the polyanion-dye ratio was approximately 1, representing typical "metachromasy," were subjected to thermodynamic analysis. These data did not fit a common equation deriving equilibrium constants spectrophotometrically by variation of reactant concentrations. This failure supported the view of metachromasy as a phenomenon primarily involving dye-dye interaction. Another equation based only on temperature variation yielded a value of –4 kcal/mole for the chondroitin sulfate-toluidine blue complex, in good agreement with similar published values obtained both in solutions and stained materials.

THE ULTRAVIOLET SPECTRA OF RIBONUCLEIC ACIDS IN SOLID STATE AND IN SOLUTION

1965 ◽  
Vol 43 (12) ◽  
pp. 3151-3159 ◽  
Author(s):  
Michael Falk
Keyword(s):  
Aqueous Solution ◽  
Solid State ◽  
Secondary Structure ◽  
Relative Humidity ◽  
Ribonucleic Acid ◽  
Room Temperature ◽  
Deoxyribonucleic Acid ◽  
Ribonucleic Acids ◽  
Solid Films ◽  
Spectral Changes

The ultraviolet absorption spectra of nine samples of soluble, ribosomal, and viral ribonucleic acid (RNA) were studied over the range 3 500 to 1 830 Å. The spectra and the spectral changes which occur upon denaturation were the same for all types of RNA examined, which suggests that their secondary structure is comparable. Dehydration of solid films of RNA at room temperature caused spectral changes resembling those which occur when RNA is heated in aqueous solution. This indicates that the dehydration of RNA, like the dehydration of deoxyribonucleic acid (DNA), causes some loss of the regular stacking and pairing of the nucleotide bases in the helical regions. In contrast to DNA, the rehydration of RNA was not always complete at 98% relative humidity. The decrease of absorbance in the 2 900 Å region upon denaturation, previously reported by Rich and Kasha, was not confirmed with any of the RNA samples studied.

scholarly journals Säurehydrolyse von Ribonucleinsäure und Desoxyribonucleinsäure

1961 ◽  
Vol 16 (10) ◽  
pp. 673-678 ◽  
Author(s):  
W. Pollmann ◽  
G. Schramm
Keyword(s):  
Enzymatic Hydrolysis ◽  
Nucleic Acids ◽  
Potentiometric Titration ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Potentiometric Method ◽  
Ribonucleic Acids ◽  
Rate Of Hydrolysis ◽  
Phosphate Groups

A method for the potentiometric titration of secondary phosphate groups in nucleic acids is described. Ribonucleic acids of yeast and of microsomes contain 5—6% secondary phosphate groups which cannot be removed by dialysis. The potentiometric method was applied to study several enzymatic hydrolyses and the non-enzymatic hydrolysis between pH 2.4 —1.8. The rate of hydrolysis for the purines and for the phosphate groups is approximately proportional to the H®-concentration. The constants of hydrolysis for ribonucleic acid and for deoxyribonucleic acid were determined. In DNA the depurinisation is 650 times faster than in RNA.

scholarly journals The Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid

1966 ◽  
Vol 241 (12) ◽  
pp. 2933-2943 ◽  
Author(s):  
Abraham Novogrodsky ◽  
Moshe Tal ◽  
Abraham Traub ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Enzymatic Phosphorylation
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