X-ray inactivation of the Escherichia coli deoxyribonucleic acid dependent ribonucleic acid polymerase in aqueous solution. II. Initiation and fidelity of transcription

Biochemistry ◽  
1973 ◽  
Vol 12 (4) ◽  
pp. 603-608 ◽  
Author(s):  
Gary F. Strniste ◽  
David A. Smith ◽  
F. Newton Hayes
Keyword(s):  
Escherichia Coli ◽  
Aqueous Solution ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
X Ray

scholarly journals A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase (Short Communication)

1973 ◽  
Vol 133 (1) ◽  
pp. 201-203 ◽  
Author(s):  
Peter Humphries ◽  
David J. McConnell ◽  
Robert L. Gordon
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Short Communication ◽  
Gel Filtration ◽  
High Salt ◽  
Rapid Procedure ◽  
Complete Purification ◽  
Rapid Purification

A rapid procedure involving DNA–cellulose chromatography followed either by sedimentation in a high-salt glycerol gradient or by gel filtration is described for the complete purification of Escherichia coli DNA-dependent RNA polymerase.

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