AN ULTRAMICRO METHOD FOR QUANTITATIVE DETERMINATION OF AMINO ACIDS

1952 ◽  
Vol 30 (8) ◽  
pp. 581-591 ◽  
Author(s):  
Eunice F. Wellington
Keyword(s):  
Amino Acids ◽  
Quantitative Method ◽  
Environmental Temperature ◽  
Complete Oxidation ◽  
Two Dimensional ◽  
Color Intensity ◽  
Color Development ◽  
Concentration Time

A simple, quantitative method for determination of amino acids in about 300 µgm. protein is described. The amino acids are separated by two-dimensional paper chromatography. The chromatograms are sprayed with 2% ninhydrin and the color is developed under controlled and standardized conditions. The colored areas are cut out, eluted, and the color intensity is read in a photometer. The following factors have been shown to affect the color reaction of ninhydrin with amino acids on paper chromatograms: the quality of the ninhydrin; its concentration; time of color development; and the environmental temperature and humidity. Complete oxidation of the hydrolyzate is necessary for accurate quantitative determinations of cysteine, cystine, glutamic acid, aspartic acid, methionine, and valine. Strict standardization of the length of the solvent run is also important for reproducibility in the analyses of some amino acids.Bovine serum albumin has been analyzed by this method and the results found to be in excellent agreement with previously published values.

Pengaruh Kualitas Layanan Terhadap Tingkat Kepuasan Masyarakat Pada Kantor Imigrasi Bengkalis

2020 ◽  
Vol 9 (1) ◽  
pp. 102-114
Author(s):  
Muhammad Fadhil Junery ◽  
Nur Asyira
Keyword(s):  
Data Collection ◽  
Service Quality ◽  
Quantitative Method ◽  
Central Government ◽  
Sampling Technique ◽  
Community Satisfaction ◽  
Convenience Sampling

This research aims to determine the immigration office services providing services to the community and how much influence the quality of immigration office services on the level of community satisfaction. This research uses a descriptive quantitative method. The population in this research amounted to 21613 people. While sampling uses the Slovin formula with convenience sampling technique so that a sample of 100 people is obtained. Data collection techniques used were observation, interviews, documentation, questionnaires and literature studies. The results of this research are that the service at the Bengkalis Immigration Office has been well fulfilled based on the determination of the central government. Then the service quality of Bengkalis Immigration Office has a significant effect on the level of community satisfaction with a percentage of influence of 68.6% and the remaining 31.4% is influenced by other variables.

AN IMPROVED METHOD FOR THE DETERMINATION OF PHOSPHATE BY PHOTOELECTRIC COLORIMETRY

1938 ◽  
Vol 16b (3) ◽  
pp. 97-108 ◽  
Author(s):  
W. J. Dyer ◽  
C. L. Wrenshall
Keyword(s):  
Organic Matter ◽  
Accurate Method ◽  
Improved Method ◽  
Color Intensity ◽  
Soil Extracts ◽  
Color Development ◽  
Light Filters ◽  
Photoelectric Colorimeter ◽  
Made In

A sensitive and accurate method for the determination of phosphate, involving the application of the Evelyn photoelectric colorimeter to the ceruleomolybdate reaction, is described. This technique makes it possible to differentiate phosphate phosphorus from other forms of phosphorus.Conditions affecting the rate and extent of color development have been studied. The results show that the maximum color intensity is developed m about five minutes after addition of the reducing agent. The determination may be made in the presence of extraneous color, and soil organic matter does not interfere with the reaction. Under the conditions specified, using light filters, Beer's law applies to the reaction in soil extracts as well as in pure solutions in the range 0.02 to 0.40 parts per million of phosphorus.

Determination of D- and L-amino acids in biological samples by two-dimensional column liquid chromatography

1995 ◽  
Vol 41 (1-2) ◽  
pp. 6-14 ◽  
Author(s):  
N. C. van de Merbel ◽  
M. Stenberg ◽  
R. Öste ◽  
G. Marko-Varga ◽  
L. Gorton ◽  
...  
Keyword(s):  
Amino Acids ◽  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biological Samples ◽  
Two Dimensional

A simple quantitative method for the determination of small amounts of amino-acids

The Analyst ◽  
1967 ◽  
Vol 92 (1099) ◽  
pp. 627 ◽  
Author(s):  
J. G. Heathcote ◽  
R. J. Washington
Keyword(s):  
Amino Acids ◽  
Quantitative Method

scholarly journals A colorimetric micro method for the determination of formyl groups

1969 ◽  
Vol 111 (4) ◽  
pp. 401-406 ◽  
Author(s):  
S. Usha Lakshmi ◽  
L. K. Ramachandran
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Chromatographic Separation ◽  
Quantitative Method ◽  
Standard Procedure ◽  
Formyl Group ◽  
Alumina Column ◽  
Purple Colour ◽  
Paper Chromatographic Separation

The characteristic purple colour formed by N-formyl-N′-2,4-dinitrophenyl-hydrazine in the presence of piperidine and acetone was made the basis of a new quantitative method for the determination of formyl groups. Samples containing N-formyl groups (up to 0·4μmole) are hydrazinolysed at 97–98° for 1hr. and are dinitrophenylated after the removal of excess of hydrazine. Interference from 2,4-dinitrophenylhydrazine is eliminated by subjecting the dinitrophenylated samples to chromatography on an alumina column. Interference arising from the formation of N-acetyl-N′-2,4-dinitrophenylhydrazine, when determining formyl groups in samples containing acetyl, can be avoided by a paper-chromatographic separation before analysis. A standard procedure is described. The method gives satisfactory results when applied to N-formyl-amino acids. Gramicidin, when analysed by this method, was found to contain 0·89 mole of formyl group/mole for a molecular weight of 1880. The method indicated the absence of formyl groups from lysozyme, a protein known not to contain such groups. Generally, the analytical values obtained by the method are within 100±4% of theory.

Determination of D- and L-amino acids in biological samples by two-dimensional column liquid chromatography

1995 ◽  
Vol 41 (5-6) ◽  
pp. 6-14 ◽  
Author(s):  
N. C. van de Merbel ◽  
M. Stenberg ◽  
R. Öste ◽  
G. Marko-Varga ◽  
L. Gorton ◽  
...  
Keyword(s):  
Amino Acids ◽  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biological Samples ◽  
Two Dimensional

scholarly journals Determination of Chiral Amino Acids in Various Fermented Products Using a Two-Dimensional HPLC-MS/MS System

2019 ◽  
Vol 40 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Chiharu ISHII ◽  
Takeyuki AKITA ◽  
Masanobu NAGANO ◽  
Masashi MITA ◽  
Kenji HAMASE
Keyword(s):  
Amino Acids ◽  
Two Dimensional ◽  
Chiral Amino Acids ◽  
Fermented Products

Evaluation of the Role of Nutrition in Lobster Recruitment

1986 ◽  
Vol 43 (11) ◽  
pp. 2320-2327 ◽  
Author(s):  
J. D. Castell ◽  
J. C. Kean
Keyword(s):  
Amino Acids ◽  
Essential Amino Acids ◽  
Field Application ◽  
Nutritional Requirements ◽  
Egg Viability ◽  
Good Opportunity ◽  
Broodstock Nutrition

Many of the hypotheses and proposals presented here on the role of nutrition in lobster recruitment have been derived from research on other aquatic animals. Little research has focused on the determination of nutritional requirements of lobsters and even that has been primarily with small juveniles (36–1000 mg). Despite the tacit acknowledgement that maternal fecundity and egg viability are a function of quantity and quality of available food, little is known about the nutrition of reproductively mature lobsters. The importance of broodstock nutrition and possible methods for evaluating nutritional status are discussed. Information on protein, essential amino acids, vitamins, minerals, carbohydrates, and lipids is summarized. Additionally, some proposals for field application of nutritional response criteria used in the laboratory are discussed. Though research on larvae adult broodstock nutrition, effects of environmental factors (e.g. temperature, salinity, photoperiod, etc.), as well as many other aspects of nutrition is yet to be done, there is "good opportunity" for nutrition to be a significant part of the current lobster recruitment research program.

PHOTOMETRIC STUDIES ON THE DIAZO REACTION FOR SERUM BILIRUBIN

1952 ◽  
Vol 30 (6) ◽  
pp. 552-560
Author(s):  
F. D. White ◽  
Dorothea Duncan
Keyword(s):  
Sodium Salt ◽  
Calibration Curve ◽  
Serum Bilirubin ◽  
Alcohol Solution ◽  
Color Intensity ◽  
Color Formation ◽  
Color Development ◽  
Beer's Law ◽  
Beer’S Law

Photometric studies on the Malloy-Evelyn procedure for the determination of serum bilirubin have shown that the azobilirubin color intensity obtained when the diazo reagent acts for 30 min. upon a known amount of bilirubin added to serum in the form of the sodium salt is less than when the reaction takes place with the same amount of bilirubin in chloroform-alcohol solution. This suggests that the usual calibration curve prepared from bilirubin in chloroform-alcohol solution, when used as standard for serum bilirubin determinations, may give values which are about 10% less than the true bilirubin content of the serum. It has also been shown that with the 1 : 10 dilution of the Malloy-Evelyn procedure the azobilirubin from icteric sera does not obey Beer’s law beyond a serum bilirubin content of 15 mgm. per 100 ml. Evidence is submitted that the rate of azobilirubin color formation can be markedly accelerated by increasing the strength of the diazo reagent, and a new reagent is proposed by the use of which the time of color development can be reduced from 30 min. to 5 min.

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